Nurses\u27 Alumnae Association Bulletin - Volume 6 Number 9

Remember the Relief Fund Welcome! Miss Childs Financial Report Calendar of Coming Events Lest You Forget! Attention Review of the Alumnae Association Meetings Institutional Staff Nurses\u27 Section Report of Staff Activities - 1947-1948 Private Duty Section The White Haven Division Barton Memorial Division Remember the Relief Fund Student Nurses\u27 Activities Jefferson Scores Again The Clara Melville Scholarship Fund Interesting Activities of the Nurses\u27 Home Committee of the Women\u27s Board Exclusive for Nurses Changes in the Maternity Division Gray Lady Musical Therapy Service Memorial Service Honoring Mrs. Bessie Dobson Altemus The Blood Donor Center The Hospital Pharmacy Medical College News Remember the Relief Fund Administrative Staff and Faculty of the School of Nursing Streptomycin Changes in the Staff at Jefferson Hospital Care of the Thoracic Surgical Patient Miscellaneous Items Marriages New Arrivals Deaths The Bulletin Committee Attention, Alumnae New Addresse

Sensitisation waves in a bidomain fire-diffuse-fire model of intracellular Ca²⁺ dynamics

We present a bidomain threshold model of intracellular calcium (Ca²⁺) dynamics in which, as suggested by recent experiments, the cytosolic threshold for Ca²⁺ liberation is modulated by the Ca²⁺ concentration in the releasing compartment. We explicitly construct stationary fronts and determine their stability using an Evans function approach. Our results show that a biologically motivated choice of a dynamic threshold, as opposed to a constant threshold, can pin stationary fronts that would otherwise be unstable. This illustrates a novel mechanism to stabilise pinned interfaces in continuous excitable systems. Our framework also allows us to compute travelling pulse solutions in closed form and systematically probe the wave speed as a function of physiologically important parameters. We find that the existence of travelling wave solutions depends on the time scale of the threshold dynamics, and that facilitating release by lowering the cytosolic threshold increases the wave speed. The construction of the Evans function for a travelling pulse shows that of the co-existing fast and slow solutions the slow one is always unstable

Microcup Arrays for the Efficient Isolation and Cloning of Cells

Arrays of transparent, releasable micron-scale structures termed “microcups” were created for the purpose of patterning and isolating viable cells from small cell samples. Cells were captured by the microcups without the need for barriers or walls on the intervening substrate. Furthermore, in contrast to prior methods for creating cell arrays with releasable elements, no chemical modification of the substrate was required. Individual microcups were released from the array using a pulsed laser at very low energy. Improvements in microcup design enabled cells in suspension to be loaded into the microcups with greater than 90% efficiency. Cells cultured within the microcups displayed 100% viability and were cultured over 4 days yielding colonies that remained sequestered within the microcups to generate pure clonal populations. Standard microscopic imaging was used to identify cells or colonies of interest, and the microcups containing these cells were then released and collected. Individual target cells isolated in this manner remained viable as demonstrated by clonal expansion of 100% of collected cells. Direct comparisons with cell isolation by fluorescence-activated cell sorting and magnetic-bead-based isolation systems demonstrated that the microcup cell isolation procedure yielded higher purity, yield and viability than these standard technologies when separating samples with small numbers of cells. The power of this technique was demonstrated by the isolation of hematopoietic stem cells from a human bone marrow aspirate possessing only 4,000 total cells

Large-Area Silicon Detectors for the Advanced Composition Explorer (ACE) Solar Isotope Spectrometer (SIS)

Extensive measurements were made of the thicknesses and dead-layers of the large-area, highpurity silicon detectors used for the Solar Isotope Spectrometer (SIS), an instrument to be launched on the Advanced Composition Explorer (ACE) spacecraft. Tests using accelerated beams of heavy nuclei were also carried out to characterize the completed instrument

How Do Information and Communication Technologies Reshape Work? Evidence from the Residential Real Estate Industry

We are exploring how information and communication technology (ICT) use affects the work lives of real estate agents, the process of selling/buying houses, and the overall structure of the residential real estate industry. Earlier stages of our work involved intensive field research on how real estate agents use ICT. In this paper, we report on the design and analysis of a pilot survey of 868 agents intended to investigate their ICT use more generally. Analysis of the 153 responses to this survey sheds light on how ICT use supports information control, enables process support, and helps agents to extend and maintain their social capital

Live Cultures: Illness, Mortality, and Masculinity in Contemporary Spanish Film

Live Cultures explores the ways that democratic Spain has been understood, written, and filmed as an ill kingdom, and how illness as bodily effect is an ontology that affects our understanding of our discrete selves. To consider illness a dark geography, as Sontag wrote in Illness as Metaphor, begs a continual re-interpretation of the relation of self, body and nation, an understanding of healthy or ill citizenship as it is inscribe into the body. This politicized inscription does not function alone but in tandem with gender, considering the ways that illness in its most base function operates some effect on the body and mind, on the notion of self as whole, complete, and functioning. Likewise, masculinity, in its role as gender practice that adheres to some concept of the `body,\u27 cannot be divorced from illness. I hold that illness is always already tied into masculinity, the two so fused together as to be inextricable. Live Cultures examines this fusion and its byproducts, those sick masculinities that are reconfigured as examples of national health, of organic space, and as carriers of contemporary violence in Spanish film. The introduction attempts to locate the germs of the project itself by beginning with Sontag\u27s classic text in illness studies and continuing with Foucault\u27s extensive work on illness as social event, and further on to more recent texts on illness as cultural, social, and gendered bodily effect. This project engages those texts that deal with the philosophical and political ramifications of illness, such as Adriana Cavarero\u27s work on the perception of the political body or Elaine Scarry\u27s Body in Pain, which is most closely interested in the representation of illness as metaphor. Live Cultures focuses on three such metaphors of illness in particular. In my first chapter, Hobbes\u27s notion of the body politic is discussed in light of select contemporary Spanish films, utilizing the filmic treatment of the body to articulate a particular Spanish conceptualization of its own nationhood. This is seen effectively in the body of Javier Bardem, which serves to represent the stately body in both its exceptionalism and its ordinariness, its sickness and health, life and death--and in the interstices where these meet. In focusing on violence as virus, as ill contagion, my second chapter explores the metaphors that frame outpourings of contemporary violence as direct effects of cross-generational malaise. In an analysis of select films of Agust?Á Villaronga, I find this viral violence and its linkage to a concept of inevitable genetics to express a modern concern with the resurging traumas of the past. Finally, by studying a selection of recent films by Pedro Almod??var, my third chapter reads the queering of death and mourning as a way of reimagining the finalizing temporalities of normative time lines. Analyzing Almod??var\u27s particular work with gender and death as touched by asynchronocity, I explore the reversal of the obliterating effects of death in favor of its productive capabilities, the creational aspects of death within life. | 172 page

Compartmentalization of Calcium Extrusion Mechanisms in the Outer and Inner Segments of Photoreceptors

AbstractDifferential localization of calcium channel subtypes in divergent regions of individual neurons strongly suggests that calcium signaling and regulation could be compartmentalized. Region-specific expression of calcium extrusion transporters would serve also to partition calcium regulation within single cells. Little is known about selective localization of the calcium extrusion transporters, nor has compartmentalized calcium regulation within single neurons been studied in detail. Sensory neurons provide an experimentally tractable preparation to investigate this functional compartmentalization. We studied calcium regulation in the outer segment (OS) and inner segment/synaptic terminal (IS/ST) regions of rods and cones. We report these areas can function as separate compartments. Moreover, ionic, pharmacological, and immunolocalization results show that a Ca-ATPase, but not the Na+/K+, Ca2+ exchanger found in the OSs, extrudes calcium from the IS/ST region. The compartmentalization of calcium regulation in the photoreceptor outer and inner segments implies that transduction and synaptic signaling can be independently controlled. Similar separation of calcium-dependent functions is likely to apply in many types of neuron