Does genetics play a role in feeding behavior of gray tree frogs?

Abstract only availableModels of sexual selection suggest that, in some animals, females choose mates of genetically superior quality. In the gray tree frog Hyla versicolor, it has been shown that females prefer male advertisement calls of long duration. Studies have also found that offspring of long-calling males have a performance advantage over the offspring of short-calling males. This research focuses on the feeding habits of H. versicolor tadpoles in an attempt to understand the contribution of paternal genetic quality to tadpole behavior. The tadpoles used in this study were offspring from long-calling males and short-calling males, reared individually in the lab. Tadpoles were individually weighed and then observed on three different occasions over the course of a week: one day after food administration, immediately after food administration, and one day after a subsequent food administration. Tadpole behavior was classified as either feeding, resting, or swimming. Results reveal that feeding behavior did not change with age, though feeding behavior was significantly higher immediately after food administration. In addition, larger tadpoles spent more time feeding than smaller tadpoles. Preliminary analyses indicate that the offspring of short-calling males were, on average, larger in size than offspring of long-calling males and, probably as a consequence of their larger size, spent more time feeding. In summary, paternal genetic quality appears to affect feeding behavior of their offspring, although further investigation is needed to determine whether tadpole feeding behavior affects performance later in life.REU Supplement to A. Welch and C. Gerhard

Are Vision-Specific Quality of Life Questionnaires Important in Assessing Rehabilitation for Patients With Hemianopia Post Stroke?

This author accepted manuscript (post print) is made available following a 12 month embargo from the date of publication (2011) in accordance with the publisher copyright policy.Objective: To explore the relationship between disability and functional measures with vision-specific quality of life (QoL) measures for people with hemianopia and stroke. Method: The Behavioral Inattention Test (BIT) and the Mayo-Portland Adaptability Inventory (MPAI) were compared with scores on 2 vision-specific QoL measures, the National Eye Institute Visual Function Questionnaire (NEI VFQ-25) and Veteran Low Vision Visual Function Questionnaire (VA LV VFQ-48). Setting: Rehabilitation hospitals in Adelaide, South Australia. Participants: Stroke patients (n = 24) with homonymous hemianopia. Results: Most of the BIT and MPAI scores were significantly associated with the NEI VFQ-25 and VA LV VFQ-48 scores. Behavioral test scores of the BIT and the MPAI total score correlated with more aspects of the QoL measures than the other components of the BIT and the MPAI. Conclusion: BIT and MPAI measure constructs associated with QoL for people with hemianopia following stroke. Vision-specific QoL questionnaires can complement the functional instruments by identifying the domains of difficulty, based on the instrument’s subscale, that can guide rehabilitation therapists to address the person’s deficit

Functional plasticity of antibacterial EndoU toxins.

Bacteria use several different secretion systems to deliver toxic EndoU ribonucleases into neighboring cells. Here, we present the first structure of a prokaryotic EndoU toxin in complex with its cognate immunity protein. The contact-dependent growth inhibition toxin CdiA-CTSTECO31 from Escherichia coli STEC_O31 adopts the eukaryotic EndoU fold and shares greatest structural homology with the nuclease domain of coronavirus Nsp15. The toxin contains a canonical His-His-Lys catalytic triad in the same arrangement as eukaryotic EndoU domains, but lacks the uridylate-specific ribonuclease activity that characterizes the superfamily. Comparative sequence analysis indicates that bacterial EndoU domains segregate into at least three major clades based on structural variations in the N-terminal subdomain. Representative EndoU nucleases from clades I and II degrade tRNA molecules with little specificity. In contrast, CdiA-CTSTECO31 and other clade III toxins are specific anticodon nucleases that cleave tRNAGlu between nucleotides C37 and m2 A38. These findings suggest that the EndoU fold is a versatile scaffold for the evolution of novel substrate specificities. Such functional plasticity may account for the widespread use of EndoU effectors by diverse inter-bacterial toxin delivery systems

A promising Start? The Local Network Fund for Children and Young People: Interim Findings from the National Evaluation

This is a summary of the interim evaluation report of the National Evaluation of the Local Network Fund (LNF) for Children and Young People. It is based on data gathered during the first phase of the evaluation (between October 2002 to December 2003). A final report of the National Evaluation will be available early in 2005. A consortium of research organisations, led by the University of Hull and including BMRB Social Research, The University of York and the University of Sheffield were commissioned in August 2002 by the-then Children and Young People’s Unit (CYPU) to carry out the evaluation

Chronic Trichuris muris infection in C57BL/6 mice causes significant changes in most microbiota and metabolome:effects reversed by pathogen clearance

Trichuris species are a globally important and prevalent group of intestinal helminth parasites, in which Trichuris muris (mouse whipworm) is an ideal model for this disease. This paper describes the first ever highly controlled and comprehensive investigation into the effects of T. muris infection on the faecal microbiota of mice and the effects on the microbiota following successful clearance of the infection. Communities were profiled using DGGE, 454 pyrosequencing, and metabolomics. Changes in microbial composition occurred between 14 and 28 days post infection, resulting in significant changes in α and β- diversity. This impact was dominated by a reduction in the diversity and abundance of Bacteroidetes, specifically Prevotella and Parabacteroides. Metabolomic analysis of stool samples of infected mice at day 41 showed significant differences to uninfected controls with a significant increase in the levels of a number of essential amino acids and a reduction in breakdown of dietary plant derived carbohydrates. The significant reduction in weight gain by infected mice probably reflects these metabolic changes and the incomplete digestion of dietary polysaccharides. Following clearance of infection the intestinal microbiota underwent additional changes gradually transitioning by day 91 towards a microbiota of an uninfected animal. These data indicate that the changes in microbiota as a consequence of infection were transitory requiring the presence of the pathogen for maintenance. Interestingly this was not observed for all of the key immune cell populations associated with chronic T. muris infection. This reflects the highly regulated chronic response and potential lasting immunological consequences of dysbiosis in the microbiota. Thus infection of T. muris causes a significant and substantial impact on intestinal microbiota and digestive function of mice with affects in long term immune regulation

From Armbands to Douchbags: How Doninger v. Niehoff Shows the Supreme Court Needs to Address Student Speech in the Cyber Age

Part II of this Note discusses the background of First Amendment student speech cases as decided by the Supreme Court as well as a unique classification of lower court holdings. Part III focuses on Doninger v. Niehoff in detail, including the underlying facts, competing arguments, procedural history, and the District of Connecticut’s and Second Circuit’s rationale. Part IV analyzes why this case was wrongly decided and argues that the Supreme Court needs to offer more guidance to lower courts so they may apply a more consistent standard in student speech cases. Further, it suggests a framework courts should adopt in considering a minor’s First Amendment rights after school hours. Part V concludes that this case is part of an emerging area of law that will continue to create mass confusion among lower courts unless the Supreme Court sets out a universally applicable and practical standard

Structure and representation at the local level : the relative effects for male and female city council members

This research examines the differences in the effects of municipal electoral structure on male versus female city council members. The question guiding this research is one of election versus performance. Two research hypotheses are advanced: RHl; More women are elected to reformed city councils than are elected to unreformed city councils. RH2: Female city council members perform better under unreformed electoral and governing structures. First, this research examines how a city\u27s electoral structure (i.e., city size, city type, election type, and type of race) affects the descriptive representation (i.e., income and educational attainment) of the men and women elected to city council. I hypothesize that male and female city council members will be drawn from the same high income bracket, regardless of electoral and governing structures. However, I predict that male city council members will be better educated than their female counterparts, regardless of electoral and governing structures. Second, this study examines council members\u27 representational foci ( i.e.. localized versus city-wide). I hypothesize that female city council members will have a more localized representational focus than their male counterparts, regardless of electoral and governing structures. Third, this study examines council members\u27 policy predispositions. The policy predisposition category is dichotomized into (1) policy - general orientations (i.e., liberal or conservative), and (2) policy substantive focus (i.e., human services, capital expenditures, and development). It is hypothesized that women will favor human services more than their male colleagues, while men favor capital expenditure and development more than their female colleagues. The data considered in this study is attitudinal data collected from city council members in five Southeastern states (Alabama, Georgia, North Carolina, South Carolina, and Tennessee) for cities 25,000 and above population

The origin and development of the social instinct /

Thesis (M.A.)--Boston University, 1912. This item was digitized by the Internet Archive

Relative mortality in U.S. Medicare beneficiaries with Parkinson disease and hip and pelvic fractures

BACKGROUND: Parkinson disease is a neurodegenerative disease that affects gait and postural stability, resulting in an increased risk of falling. The purpose of this study was to estimate mortality associated with demographic factors after hip or pelvic (hip/pelvic) fracture in people with Parkinson disease. A secondary goal was to compare the mortality associated with Parkinson disease to that associated with other common medical conditions in patients with hip/pelvic fracture. METHODS: This was a retrospective observational cohort study of 1,980,401 elderly Medicare beneficiaries diagnosed with hip/pelvic fracture from 2000 to 2005 who were identified with use of the Beneficiary Annual Summary File. The race/ethnicity distribution of the sample was white (93.2%), black (3.8%), Hispanic (1.2%), and Asian (0.6%). Individuals with Parkinson disease (131,215) were identified with use of outpatient and carrier claims. Cox proportional hazards models were used to estimate the risk of death associated with demographic and clinical variables and to compare mortality after hip/pelvic fracture between patients with Parkinson disease and those with other medical conditions associated with high mortality after hip/pelvic fracture, after adjustment for race/ethnicity, sex, age, and modified Charlson comorbidity score. RESULTS: Among those with Parkinson disease, women had lower mortality after hip/pelvic fracture than men (adjusted hazard ratio [HR] = 0.63, 95% confidence interval [CI]) = 0.62 to 0.64), after adjustment for covariates. Compared with whites, blacks had a higher (HR = 1.12, 95% CI = 1.09 to 1.16) and Hispanics had a lower (HR = 0.87, 95% CI = 0.81 to 0.95) mortality, after adjustment for covariates. Overall, the adjusted mortality rate after hip/pelvic fracture in individuals with Parkinson disease (HR = 2.41, 95% CI = 2.37 to 2.46) was substantially elevated compared with those without the disease, a finding similar to the increased mortality associated with a diagnosis of dementia (HR = 2.73, 95% CI = 2.68 to 2.79), kidney disease (HR = 2.66, 95% CI = 2.60 to 2.72), and chronic obstructive pulmonary disease (HR = 2.48, 95% CI = 2.43 to 2.53). CONCLUSIONS: Mortality after hip/pelvic fracture in Parkinson disease varies according to demographic factors. Mortality after hip/pelvic fracture is substantially increased among those with Parkinson disease. LEVEL OF EVIDENCE: Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence

A Leptin-regulated Circuit Controls Glucose Mobilization During Noxious Stimuli

Adipocytes secrete the hormone leptin to signal the sufficiency of energy stores. Reductions in circulating leptin concentrations reflect a negative energy balance, which augments sympathetic nervous system (SNS) activation in response to metabolically demanding emergencies. This process ensures adequate glucose mobilization despite low energy stores. We report that leptin receptor–expressing neurons (LepRb neurons) in the periaqueductal gray (PAG), the largest population of LepRb neurons in the brain stem, mediate this process. Application of noxious stimuli, which often signal the need to mobilize glucose to support an appropriate response, activated PAG LepRb neurons, which project to and activate parabrachial nucleus (PBN) neurons that control SNS activation and glucose mobilization. Furthermore, activating PAG LepRb neurons increased SNS activity and blood glucose concentrations, while ablating LepRb in PAG neurons augmented glucose mobilization in response to noxious stimuli. Thus, decreased leptin action on PAG LepRb neurons augments the autonomic response to noxious stimuli, ensuring sufficient glucose mobilization during periods of acute demand in the face of diminished energy stores