The heart truth campaign: a communication audit

To understand how organizations spread campaign messages, which directly relates to the theory of agenda building, a communication audit of the Heart Truth campaign and its Red Dress symbol was conducted. Through document analysis of campaign materials and interviews with campaign planners, an in-depth examination of the campaign’s planning, strategies, tactics, executions, challenges, and successes are presented. Discussion of these elements as well as a SWOT analysis reveals the agenda-building strategies that were most successful for this campaign, which include awareness events, partnership building, grassroots outreach, and media outreach. The campaign audit concludes with a set of recommendations for moving forward with the campaign, and describes how this modernday campaign is an excellent model of agenda building that other groups can emulate

A 40-weeks ovarian pregnancy: a case report

Ovarian pregnancy is a rare ectopic pregnancy, often misdiagnosed at advanced gestational age. We present a case of a 38-year-old woman at 40 weeks with ovarian pregnancy and fetal death, discovered during laparotomy at Mirebalais Teaching Hospital, Haiti. The patient presented with abdominal pain, light vaginal bleeding, and high blood pressure. Ultrasound suggested intrauterine fetal death and placenta previa, but the laparotomy revealed a non-gravid uterus and a large amniotic sac in the left ovary, suggesting a term ovarian pregnancy. A left adnexectomy was performed, and histopathology confirmed the diagnosis based on the Spiegelberg criteria. Despite complications, the patient had a good outcome. This case emphasizes the diagnostic challenges and the importance of early prenatal care to prevent complications life-threatening associated with advanced ovarian pregnancies

Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene

Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact NANOS2 allele and female knockout pigs are fertile. From an agriculture perspective, NANOS2 knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires

The investigation of learning within a nursing preceptorship clinical experience: a naturalistic inquiry

textCurriculum and Instructio

PUBERTAL DEVELOPMENT OF THE BOAR: AGE-RELATED CHANGES IN TESTICULAR MORPHOLOGY AND SELECTED ENDOCRINE PARAMETERS

Characterization of the pubertal development of the boar was investigated by monitoring various morphological and endocrinological parameters. Forty-eight Landrace X Duroc boars were assigned at weaning to one of eight castration ages (40, 70, 100, 130, 160, 190, 220 and 250 days). Blood samples were collected every 1/2-h between 0800 and 1200 h 2 days before (-2) and on days +1, +2, +3, +4, +8 and +16 after castration. Serum concentrations of testosterone (T), estradiol-17(beta) (E(,2)), cortisol (C) and luteinizing hormone (LH) were quantified by specific radioimmunoassays. The left testis was perfused with glutaraldehyde and processed for histological examination. Portions of the right testis were immediately prepared for a 3-h in vitro incubation procedure to assess T production as a response to varying dosages of human chorionic gonadotropin (hCG). Mean serum concentrations of T and E(,2) increased (P \u3c .01) in a near linear fashion with age of boar. Mean serum concentrations of T and E(,2) were 1.31, 2.24, 4.03, 9.99, 10.92, 15.75, 15.76 and 8.66 ng/ml and 11.38, 19.15, 15.75, 52.90, 89.27, 97.80, 107.95 and 118.11 pg/ml, respectively, for boars 40, 70, 100, 130, 160, 190, 220 and 250 days of age. Mean concentrations of LH before castration (day -2) did not differ with age (overall mean of 1.06 ng/ml) while serum LH concentrations were elevated (P \u3c .05) within one to two days following castration at 40, 70, 100, 130 and 160 days of age. Testicular volume percentage of seminiferous tubules increased from 36% to 72% between 40 and 250 days of age. Testicular volume percentage of Leydig cells decreased from 40% to 10% between 40 and 250 days of age. Leydig cells from boars 130 and 160 days of age had the greatest (P \u3c .01) capacity of T production when evaluated at 3125 mIU hCG/ml incubation media. Sensitivity, as judged by ED(,50) calculations, was markedly increased (P \u3c .05) in testicular tissue from boars 130 days of age and older. In conclusion, the data indicate that one factor responsible for pubertal development of the boar is an increased sensitivity of the testes to gonadotropic stimulation