Large deep-seated gravitational slide off Ischia volcanic island, Eastern Tyrrhenian sea (Italy)

Ischia island develops at the edge of the shelf area and represents the sub-aerial section of a larger, E-W trending volcanic ridge including others submerged or buried volcanic edifices. In the past decade the island’s offshore has been the object of extensive hydrographic and marine geophysical surveys that have shown the structural complexity of the undersea sections and have overall shown the importance of gravity failures in island’s evolution. In this paper we report a previously unreported deep-seated slump structure and associated surficial mass wasting phenomena which occur off Ischia south-western flank. Recently acquired hydrological and geophysical data lead to identify the morphological features and the internal organization of the failed sediments which spread along the continental slope. The extent of this deep-seated deformations and the deep structural levels involved lead to investigate on the influence played by volcanic processes and regional tectonics on slope failure. The Ischia southern slope was explored through a multibeam survey and a single-channel seismic survey. Acquisition was carried out from aboard the R/V Urania at depths between 400 and 1200 m. The bathymetric data were collected using a hull mounted Reson 8160 multibeam sonar. Resolution resulted in a 20x20 m implemented with 50x50 gridded size provided by a previously collected data. Seismic survey consisted of 6 seismic lines run along the slope spaced 1 km between them and 5 cross lines. The acoustic source used was a 1Kjoule high-energy power supply system with a multi-tips (400) sparker array, fired at 2s time interval. The collected data show that a wide submerged area of 350 km2, between 400 to 1200 m depths is undergoing slow-moving deformation and associated secondary mass wasting phenomena. Morphological features include trenches, counterscarps, bulging and both extensional and contractional features while internal deformations show typical landward dipping reflectors with strong evidence of synsedimentary faulting and asymmetric anticlines. Deformation processes operate at various scales generating folds with wavelength ranging from hundreds meters to kilometers. Extensional and rotational rupture surfaces sole out at various low-angle detachment planes located at depths from few hundreds meters to 1 kilometer in subsurface

Eradication of Candida albicans persister cell biofilm by the membranotropic peptide gH625

Biofilm formation poses an important clinical trouble due to resistance to antimicrobial agents; therefore, there is an urgent demand for new antibiofilm strategies that focus on the use of alternative compounds also in combination with conventional drugs. Drug-tolerant persisters are present in Candida albicans biofilms and are detected following treatment with high doses of amphotericin B. In this study, persisters were found in biofilms treated with amphotericin B of two clinical isolate strains, and were capable to form a new biofilm in situ. We investigated the possibility of eradicating persister-derived biofilms from these two Candida albicans strains, using the peptide gH625 analogue (gH625-M). Confocal microscopy studies allowed us to characterize the persister-derived biofilm and understand the mechanism of interaction of gH625-M with the biofilm. These findings confirm that persisters may be responsible for Candida biofilm survival, and prove that gH625-M was very effective in eradicating persister-derived biofilms both alone and in combination with conventional antifungals, mainly strengthening the antibiofilm activity of fluconazole and 5-flucytosine. Our strategy advances our insights into the development of effective antibiofilm therapeutic approaches

Toxicity Effects of Functionalized Quantum Dots, Gold and Polystyrene Nanoparticles on Target Aquatic Biological Models: A Review

Nano-based products are widespread in several sectors, including textiles,medical-products, cosmetics, paints and plastics. Nanosafety and safe-by-design are driving nanoparticle (NP) production and applications through NP functionalization (@NPs). Indeed, @NPs frequently present biological effects that differ fromthe parentmaterial. This paper reviews the impact of quantumdots (QDs), gold nanoparticles (AuNPs), and polystyrene-coredNPs (PSNPs), evidencing the role ofNP functionalization in toxicity definition. Key biological models were taken into consideration for NP evaluation: Saccharomyces cerevisiae, fresh- (F) and saltwater (S)microalgae (Raphidocelis subcapitata (F), Scenedesmus obliquus (F) and Chlorella spp. (F), and Phaeodactylum tricornutum (S)), Daphnia magna, and Xenopus laevis. QDs are quite widespread in technological devices, and they are known to induce genotoxicity and oxidative stress that can drastically change according to the coating employed. For example, AuNPs are frequently functionalized with antimicrobial peptides, which is shown to both increase their activity and decrease the relative environmental toxicity. P-NPs are frequently coated with NH2 for cationic and COOH for anionic surfaces, but when positively charged toxicity effects can be observed. Careful assessment of functionalized and non-functionalized NPs is compulsory to also understand their potential direct and indirect effects when the coating is removed or degraded

A New Light on Vitamin D in Obesity: A Novel Association with Trimethylamine-N-Oxide (TMAO)

Abstract: Vitamin D deficiency and obesity are two public health problems extensively exacerbated over the last years. Among the several mechanisms proposed to account for the complex interplay between vitamin D and obesity, one that has gained particular attention is related to the emerging role of obesity-related changes in gut microbiota and gut-derived metabolites, such as Trimethylamine-N-oxide (TMAO). Vitamin D deficiency and high circulating TMAO levels are associated with body weight and the severity of non-alcoholic fatty liver disease (NAFLD). Considering the link of obesity with vitamin D on the one hand and obesity with TMAO on the other hand, and the central role of the liver in both the vitamin D and TMAO metabolism, the aim of this cross-sectional observational study was first, to confirm the possible inverse association between vitamin D and TMAO across different body mass index (BMI) classes and second, to investigate if this association could be influenced by the presence of NAFLD. One hundred and four adult subjects (50 males and 54 females; 35.38 ± 7.49 years) were enrolled. The fatty liver index (FLI) was used as a proxy for the diagnosis of NAFLD. Vitamin D deficiency was found in 65 participants (62.5%), while 33 subjects (31.7%) had insufficient levels, and the remaining subjects had sufficient levels of vitamin D. Subjects with both vitamin D deficiency and FLI-NAFLD had the highest TMAO levels (p < 0.001). By stratifying the sample population according to the BMI classes, vitamin D levels decreased significantly along with the increase of plasma TMAO concentrations, with the lowest vitamin D levels and highest TMAO, respectively, in class III obesity. Vitamin D levels showed significant opposite associations with circulating levels of TMAO (r = -0.588, p < 0.001), but this association was no longer significant after the adjustment for FLI values. The highest values of TMAO were significantly associated with the severity of obesity (OR 7.92; p < 0.001), deficiency of vitamin D (OR 1.62; p < 0.001), and FLI-NAFLD (OR 3.79; p < 0.001). The most sensitive and specific cut-off for vitamin D to predict the circulating levels of TMAO was ≤19.83 ng/mL (p < 0.001). In conclusion, our study suggests that high TMAO levels are associated with vitamin D deficiency and NAFLD. Further studies are required to investigate if there is a causality link or whether all of them are simply the consequence of obesity

Phase angle: A possible biomarker to quantify inflammation in subjects with obesity and 25(OH)D deficiency

Obesity is associated to chronic low-grade metabolic inflammation and hypovitaminosis D. Among extra-skeletal effects, an important role in inflammation has been described for vitamin D (25(OH)D). Phase angle (PhA) is a bioelectrical impedance analysis (BIA) parameter that represents an indicator of cellular health in chronic inflammatory states. However, it is still unknown whether a low 25(OH)D levels might correlate with PhA in obesity. Considering the lack of evidence correlating the 25(OH)D levels with PhA in obesity, the aim of this study was to investigate their possible relationship in a group of patients with obesity stratified according to body mass index (BMI) categories. Four hundred and fifty-five adult subjects (219 males and 236 females; 36 ± 11 years) were enrolled. Body composition, including PhA, was assessed using a BIA phase-sensitive system. Serum levels of 25(OH)D was determined by a direct competitive chemiluminescence immunoassay. Most of the participants were affected by grade III obesity (24%) and had 25(OH)D deficiency (67%). Subjects with 25(OH)D deficiency had highest BMI (p 14 ng/mL (p < 0.001). In conclusion, we provided preliminary insights into a novel link between 25(OH)D levels and PhA in the setting of obesity. This association uncovered a new potential usefulness of PhA as expression of cell membrane integrity and predictor of inflammation in low 25(OH)D status that might help in identifying high-risk patients with obesity who could benefit from careful 25(OH)D supplementation

Trimethylamine-N-oxide (TMAO) as novel potential biomarker of early predictors of metabolic syndrome

There is a mechanistic link between the gut-derived metabolite trimethylamine-N-oxide (TMAO) and obesity-related diseases, suggesting that the TMAO pathway may also be linked to the pathogenesis of obesity. The Visceral Adiposity Index (VAI), a gender-specific indicator of adipose dysfunction, and the Fatty Liver Index (FLI), a predictor of non-alcoholic fatty liver disease (NAFLD), are early predictors of metabolic syndrome (MetS). In this cross-sectional observational study, we investigated TMAO levels in adults stratified according to Body Mass Index (BMI) and the association of TMAO with VAI and FLI. One hundred and thirty-seven adult subjects (59 males; 21⁻56 years) were enrolled. TMAO levels were detected using HPLC/MS analysis. Homeostatic Model Assessment of Insulin Resistance (HoMA-IR), VAI and FLI were included as cardio-metabolic indices. TMAO levels increased along with BMI and were positively associated with VAI and FLI, independently, on common potential covariates. The most sensitive and specific cut-offs for circulating levels of TMAO to predict the presence of NAFLD-FLI and MetS were ≥8.02 µM and ≥8.74 µM, respectively. These findings allow us to hypothesize a role of TMAO as an early biomarker of adipose dysfunction and NAFLD-FLI in all borderline conditions in which overt MetS is not present, and suggest that a specific cut-off of TMAO might help in identifying subjects at high risk of NAFLD

Active oceanic spreading in the northern north Fiji basin : results of the NOFI cruise of R/V l'Atalante (Newstarmer project)

The South Pandora and the Tripartite Ridges are active spreading centers located in the northern part of the North Fiji Basin. These spreading centers were surveyed over a distance of 750 km during the NOFI cruise of R/V L'Atalante (August-September 1994) which was conducted in the frame of the french-japanese Newstarmer cooperation project. SIMRAD EM12-dual full coverage swath bathymetric and imagery data as well as airgun 6-channel seismic, magnetics and gravity profiles were recorded along an off-axis from 170°40'E to 178°E. Dredging and piston coring were also performed along and off-axis. The axial domain of the South Pandora Ridge is divided into 5 first-order segments characterized by contrasted morphologies. The average width of the active domain is 20 km and corresponds either to bathymetric highs or to deep elongated grabens. The bathymetric highs are volcanic constructions, locally faulted and rifted, which can obstruct totally the axial valley. The grabens show the typical morphology of slow spreading axes, with two steep walls flanking a deep axial valley. Elongated lateral ridges may be present on both sides of the grabens. Numerous volcanoes, up to several kilometers in diameter, occur on both flanks of the South Pandora Ridge. The Tripartite Ridge consists of three main segments showing a sigmoid shape. Major changes in the direction of the active zones are observed at the segment discontinuities. These discontinuities show various geometrical patterns which suggest complex transform relay zones. Preliminary analysis of seismic reflection profiles suggest that the Tripartite Ridge is a very young feature which propagates into an older oceanic domain characterized by a significant sedimentary cover. By contrast, a very thin to absent sedimentary cover is observed about 100 km on both flanks of the South Pandora Ridge active axis. The magnetic anomaly profiles give evidence of long and continuous lineations, parallel to the South Pandora Ridge spreading axis. (Résumé d'auteur

Large deep-seated slump structure off Ischia volcanic island, Eastern Tyrrhenian sea (Italy)

Ischia island is located over the Campania sector of Eastern Tyrrhenian margin and represents the sub-aerial section of a larger, E-W trending volcanic ridge including others submerged or buried volcanic edifices. The island itself result from the coalescence of a multitude of small to medium scale eruptions leading to the emplacement of domes, lava flow and pyroclastic deposits and ignimbrites (VEZZOLI et al., 1988) ranging from alkali basalts to trachytes. The oldest basement dates back to 150 ky and crops out along the perimeter of the island especially to the south. Latest eruption occurred in 1302 A.D. and together with strong hydrothermal activity, ground uplift and seismic shaking indicates the presence of a still active magmatic reservoir at depth. Most recent (Holocene) magmatic activity with local volcanic eruptions has clustered in the eastern island’s sector the while central sector is dominated by the Mt. Epomeo, consisting of an ignimbritic tuff (Green tuff Auct.) uplifted of 600-700 m in the past 33ka. In the past decade the island’s offshore has been the object of extensive hydrographic and marine geophysical surveys that have shown the structural complexity of the undersea sections and have overall shown the importance of gravity failures in island’s evolution. In particular a 1.5-3 km3 debris avalanche due to a subaerial and/or submarine flank collapse was emplaced along the steep and unbuttressed island’s flank during pre-historical or even historical times (CHIOCCI & DE ALTERIIS, 2006; de Alteriis et al., 2010) whereas three other similar deposits of comparable volumes were found over the continental shelf to the west and to the north (VIOLANTE et al, 2004; DE ALTERIIS & VIOLANTE, 2009). Here we report a previously unrecognized deep-seated slump structure and associated surficial mass wasting phenomena which occur off Ischia south-western flank. Recently acquired hydrological and geophysical data lead to identify the morphological features and the internal organization of the failed sediments which spread along the continental slope. The extent of this deep-seated deformations and the deep structural levels involved lead to investigate on the influence played by volcanic processes on slope failure. DATA AND METHODS Our dataset was acquired during the geophysical cruise PECOS 2010 carried out on R/V Urania (Consiglio Nazionale delle Ricerche, CNR, Italy) between December 22th 2010 and January 2nd 2011 in the frame of a project leaded by Istituto per l’Ambiente Marino Costiero, (IAMC-CNR), Naples-Italy with the collaboration of Dipartimento di Scienze della Terra e del Mare (Palermo University), Palermo-Italy regarding coastal and offshore slope instability in the Bay of Napoli. The Ischia southern slope was explored through a multibeam survey and a single-channel seismic survey. Acquisition was carried out between 400 and 1200 m. The bathymetric data were collected using a hull mounted Reson 8160 multibeam sonar. Resolution resulted in a 20x20 m implemented with 50x50 gridded size provided by a previously collected data. The seismic survey consisted of 6 dip-lines NNE-SSW run along the slope and 5 cross lines parallel to the slope totalling 170 km. Average spacing between diplines was slightly less than 1 km while spacing between crosslines was variable from 1.2 to 2.5 km. NNE-SSW and WNW-ESE directions. The acoustic source used was a 1Kjoule high-energy power supply system with a multitips (400) sparker array, fired at 2s time interval. RESULTS The collected data show that a wide submerged area of 350 km2, between 400 to 1200 m depths is undergoing slow-moving deformation and associated secondary mass wasting phenomena. Morphological features include trenches, counterscarps, bulging and both extensional and contractional features while internal deformations show typical landward dipping reflectors with strong evidence of synsedimentary faulting and asymmetric anticlines. Deformation processes operate at various scales generating folds with wavelength ranging from hundreds meters to kilometers. Extensional and rotational rupture surfaces sole out at various low-angle detachment planes located at depths from few hundred meters to 1 kilometer in subsurface. The internal organization of the failing mass shows different pattern of deformation that allows the identification of three main units: 1) a basal unit consisting of a very broad, asymmetric slump fold with a wavelength of about 5 km and amplitude of some 100 m. The fold axis is not vertical and the three dimensional interpretation indicates that the structure is not cylindrical. The fold strictly correlates with a morphological bulge seen on bathymetry at about 20 km south of Ischia Island. 2) A wedge shaped intermediate unit characterized by discontinuous and folded reflectors, locally showing basal detachment planes and compressional features. 3) A surficial slump unit affecting the upper and middle slope characterized by a basal decollément surface and normal growth faults that sole out at depths ranging from 70 to 40 m in subsurface. It is still unclear whether the landslide process can be favored by the volcano-tectonic evolution and rapid vertical accretion of Ischia volcano or is solely due to possibly volcanic spreading of the Ischia Island. REFERENCES CHIOCCI, F. L. & DE ALTERIIS, G. (2006) - The Ischia debris avalanche. First, clear submarine evidence in the Mediterranean of a volcanic island pre-historic collapse. Terra Nova, 18, 202–209. DE ALTERIIS, G., INSINGA D. ET AL. (2010) - Age of submarine debris avalanches and tephrostratigraphy offshore Ischia Island, Tyrrhenian Sea, Italy. Marine Geology 278 (2010) 1– 18. DE ALTERIIS, G. & VIOLANTE, C. (2009) - Catastrophic landslides off Ischia volcanic island (Italy) during prehistory. In: C. Violante, (ed.) Geohazard in Rocky Coastal Areas. Geological Society, London, Special Publications, 322, 73– 104. VEZZOLI, L. (1988) Island of Ischia. Quaderni de ‘La Ricerca Scientifica’ Progetto finalizzato ‘Geodinamica’, CNR Monografie finali, 10. VIOLANTE, C., BUDILLON, F., ET AL. (2004) - Submerged hummocky topographies and relations with landslides on the northwestern flank of Ischia island, southern Italy. In: ‘Occurrence and mechanisms of flow-like landslides in natural slopes and earthfills’, Sorrento, 14–16 May 2003. AGI, 2, 309–315