Spleen Tyrosine Kinase Inhibition Modulates p53 Activity

Spleen tyrosine kinase (SYK) is a cytoplasmic enzyme that promotes survival and proliferation of B cells. SYK inhibition has shown promising results in the treatment of arthritis and chronic lymphocytic leukemia (CLL). However, in other context, it has been shown that SYK overexpression in epithelial cancer cells induced senescence in p53-dependent mechanism, which underscored its antineoplastic activity in vitro. Here, we show that SYK was induced in response of DNA damage in parallel with p53 levels. In addition, using chemical inhibitors of SYK reduced p53 levels in HCT116 and HT1080 cell lines, which underlines the role of SYK inhibition on p53 activity. Furthermore, SYK inhibition modulated the cell growth, which resulted in a decreasing in cell death. Interestingly, SYK expression showed a positive prognosis in patients with solid tumors in correlations with their survival rates, as expected negative correlation was seen between SYK expression and survival rate of patients with CLL. In conclusion, these findings demonstrate that SYK inhibition modulates p53 expression and activity in HCT116 and HT1080 cells. Reconsidering using of SYK inhibitors in clinical setting in the future should be evaluated carefully in accordance with these findings to prevent the formation of secondary malignancies. </jats:p

Analyzing HTTP requests for web intrusion detection

Many web application security problems related to intrusion have resulted from the rapid development of web applications. To reduce the risk of web application problems, web application developers need to take measures to write secure applications to prevent known attacks. When such measures fail, it is important to detect such attacks and find the source of the attacks to reduce the estimated risks. Intrusion detection is one of the powerful techniques designed to identify and prevent harm to the system. Most defensive techniques in Web Intrusion Systems are not able to deal with the complexity of cyber-attacks in web applications. However, machine learning approaches could help to detect known and unknown web application attacks. In this paper, we present machine learning techniques to classify the HTTP requests in the well-known dataset CSIC 2010 HTTP (Giménez et al., 2012) as normal or abnormal traffic, and we compare our experimental results with the results reported by Pham et al. in 2016 and Nguyen et al. in 2011. These experiments produce results for overlapping sets of machine-learning techniques and different sets of features, allowing us to compare how good the various feature sets are for the various machine-learning techniques, at least on this dataset. Keywords: intrusion detection system; anomaly detection; web application attacks; machine learning

BTK Modulates p53 Activity to Enhance Apoptotic and Senescent Responses

p53 is a tumor suppressor that prevents the emergence of transformed cells by inducing apoptosis or senescence, among other responses. Its functions are regulated tightly by posttranslational modifications. Here we show that Bruton's tyrosine kinase (BTK) is a novel modulator of p53. We found that BTK is induced in response to DNA damage and p53 activation. BTK induction leads to p53 phosphorylation, which constitutes a positive feedback loop that increases p53 protein levels and enhances the transactivation of its target genes in response to stress. Inhibiting BTK reduced both p53-dependent senescence and apoptosis. Further, BTK expression also upregulated DNA damage signals and apoptosis. We conclude that despite being involved in oncogenic signals in blood malignancies, BTK has antineoplastic properties in other contexts, such as the enhancement of p53's tumor suppressor responses. Along with evidence that BTK expression correlates with good prognosis in some epithelial tumors, our findings may encourage a reevaluation of the clinical uses of BTK inhibitors in cancer therapy

Graduate Medical Students’ Mental Health Concerns During COVID-19 Pandemic

Medical students are more likely than the general population to experience perceived stress during the pandemic due to many variables. This study aimed to evaluate the stress levels and prevalence of different mental health conditions among graduate medical students in Al Kharj City. An anonymous online survey was conducted among graduate medical students of Prince Sattam bin Abdulaziz University (PSAU). For this investigation, the following scales were used to measure the prevalence of common mental health issues: DSM-5 Self-Rated Level 1 Cross-Cutting Symptom Measure-Adult (CCSM-A); Perceived Stress Scale (PSS-10-C), to measure COVID-19-related student stress; and the COVID-19 Student Stress Questionnaire to get the global stress score (GSS). Two hundred twenty-one students were contacted, and 214(96.8%) consented to participate in the study. According to the CCSM-A scale, anxiety (73%) and depressive symptoms (71%) were the most frequently reported symptoms by the students. After correcting for age and self-perceived COVID-19 risk, there was a significant relationship between anger, suicidal ideation, and substance use, on one hand, and the study year on the other graduate medical students who have mental health issues bear a heavy load. In the post-pandemic recovery period, regular mental health assessments and providing early and adequate mental health assistance to needy people are imperative

Laser-assisted decay spectroscopy for the ground states of 180,182Au^180,182Au

status: publishe

Hyperfine anomaly in gold and magnetic moments of Ipi=11/2−I^pi=11/2^- gold isomers

status: publishe

Laser-assisted decay spectroscopy and mass spectrometry of 178Au^178Au

A comprehensive study of the isotope 178Au has been made at the CERN-ISOLDE facility, using resonance laser ionization. Two long-lived states in 178Au were identified—a low-spin ground state and a high-spin isomer—each of which were produced as pure beams. Using the ISOLTRAP precision Penning trap, the excitation energy of the isomeric state in 178Au was determined to be E∗=189(14)keV. The α-decay fine structure patterns of the two states were studied using the Windmill decay station, providing information on the low-lying states in the daughter nucleus 174Ir. Nuclear spin assignments of I(178Aug)=(2,3) and I(178Aum)=(7,8) are made based on the observed β-decay feeding and hyperfine structure intensity patterns. These spin assignments are used for fitting the hyperfine structures of the two states from which values for the magnetic dipole moments are extracted. The extracted moments are compared with calculations using additivity relations to establish the most probable configurations for 178Aug,m

Clinical and Imaging Trends of Ankle Sprains in Al Kharj City

Background: When the ligaments supporting the ankle become excessively stretched or torn, it results in an ankle sprain. It can occur when you awkwardly put your weight down on your foot, step in a hole, or twist your ankle when jogging or walking. The present study aimed to determine the incidence of ankle sprains and trends in advanced imaging among residents of Al Kharj. Material and Methods: This study represents a cross-section starting from June 2024 to December 2024. An evaluation of the clinical presentation and imaging trends in patients with ankle sprains was collected from three hospitals in Al Kharj (King Khaled, Aflag General, and Prince Sattam bin Abdulaziz University Hospitals). The study included 69 patients (88.4% males and 11.6% females) of different age groups and sprain severity. The clinical data included patient demographics (age, sex), the side of injury, Saudi or non-Saudi nationality, the results of the physical examination, and the treatment options. Imaging data included CT, MRI, or X-ray. Results: The largest age group according to age distribution was 12–20 years (39.1%), followed by 21–29 years (20.2%) and 30–39 years (17.3%). Ankle sprains were caused by sports injuries in 40.6% of cases, falls in 23.2%, and walking-related accidents in 18.8% of cases (P=0.0240). Imaging procedures of different ankle sprain cases showed that X-ray was the most common (92.7%), followed by MRI (34.7%), and ultrasonography (17.3%) (P<0.0001). Our results showed that 17.3% of cases received no treatment, 53.6% received medical treatment and slab or crepe bandage, and 21.7% had medical treatment and slab or crepe bandage followed by physiotherapy. Additionally, 7.2% had surgery to repair a ligament injury after an MRI confirmed the diagnosis. Conclusion: Ankle sprains are more common in younger people. Most of the time, conservative initial treatment (watchful waiting) is a safe option for initial injury management