How can companies rebalance their supply chains to reduce their reliance on China? - the case of Burberry

This academic thesis aims to explore strategies by which companies can reduce dependency on China in their supply chains. We combined quantitative and qualitative analyses to understand what is driving China's significance, underlying awareness of rebalancing, associated risks, and effective approaches to reduce dependence. A survey and interviews were conducted to provide meaningful findings to support our recommendations and conclusions. The case studies highlighted several practical suggestions, including diversified supplier bases, off-and nearshoring and increased digital integration for risk management for more resilient global supply chains

Классификация экзонов генов человека с использованием алгоритмов автоматического выбора атрибутов экзонов

Volkov AV, Yatskov NN, Grinev VV Classification of exons of human genes using algorithms for automatic selection of exon attributesСекция 2. МЕТОДЫ И ТЕХНОЛОГИИ МАТЕМАТИЧЕСКОГО И ИМИТАЦИОННОГО МОДЕЛИРОВАНИЯ СИСТЕ

Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.

The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines designed for nervous system-specific recombination. Selective maternal or paternal germline recombination is showcased with sample Cre lines. Collated data reveal germline recombination in over half of 64 commonly used Cre driver lines, in most cases with a parental sex bias related to Cre expression in sperm or oocytes. Slight differences among Cre driver lines utilizing common transcriptional control elements affect germline recombination rates. Specific target loci demonstrated differential recombination; thus, reporters are not reliable proxies for another locus of interest. Similar principles apply to other recombinase systems and other genetically targeted organisms. We hereby draw attention to the prevalence of germline recombination and provide guidelines to inform future research for the neuroscience and broader molecular genetics communities

Extracting periodically repeating shocks in a gearbox from simultaneously occurring random vibration

AbstractPeriodically repeating shocks are a quite common indication of certain defect in machinery. Detecting these shocks in early stage, before the defect is severe enough to cause failure, can provide a huge advantage in maintenance planning. The earliest possible warning of a defect may be highly important, especially in targets where failure can lead to a vast loss of production or a safety risk. Shock-like vibrations are, however, usually rather faint when the defect is a minor one. This simply means that the shocks may often be too low in magnitude to be easily detected. In this paper, we use different techniques based on real order derivative to detect gear defects. Higher real order derivative, discrete Fourier transform, and Hilbert transform are discussed.Abstract Periodically repeating shocks are a quite common indication of certain defect in machinery. Detecting these shocks in early stage, before the defect is severe enough to cause failure, can provide a huge advantage in maintenance planning. The earliest possible warning of a defect may be highly important, especially in targets where failure can lead to a vast loss of production or a safety risk. Shock-like vibrations are, however, usually rather faint when the defect is a minor one. This simply means that the shocks may often be too low in magnitude to be easily detected. In this paper, we use different techniques based on real order derivative to detect gear defects. Higher real order derivative, discrete Fourier transform, and Hilbert transform are discussed

Performance Analysis of Piezoelectric Energy Harvesting System.

[EN] This paper analyzes a piezoelectric system made of a smart lead zirconate material. The system is composed of a monolithic PZT (piezoelectric ceramic) plate made of a ceramic-based piezoelectric material. The experiment was conducted on a test stand with a GUNT HM170 wind tunnel and a special measurement system. The developed bluff-body shape mounted on an elastic beam with a piezoelectric was mounted on a mast with arms. Springs were fixed on the arms to limit the movement of the test object. Air flow velocity in the wind tunnel and forced vibration frequencies were changed during the tests. The recorded parameters were an output voltage signal from the piezoelectric element and linear accelerations at selected points of the test object. The highest energy efficiency of the tested system was specified from mechanical vibrations and air flow. The results of the tests are a resonance curve for the tested system and a correlation of RMS voltage and acceleration as a function of the velocity of air flow for the excitation frequency f ranging from 1 to 6 Hz. The tests specified the area where the highest output voltage under the given excitation conditions is generated.Publication was supported by the program of the Polish Ministry of Science and Higher Education under the project DIALOG 0019/DLG/2019/10 in the years 2019¿2021.Ambrozkiewicz, B.; Czyz, Z.; Staczek, P.; Tiseira, A.; Garcia Tiscar, J. (2022). Performance Analysis of Piezoelectric Energy Harvesting System. Advances in Science and Technology Research Journal. 16:179-185. https://doi.org/10.12913/22998624/1562151791851

Srsf1 and Elavl1 act antagonistically on neuronal fate choice in the developing neocortex by controlling TrkC receptor isoform expression

The seat of higher-order cognitive abilities in mammals, the neocortex, is a complex structure, organized in several layers. The different subtypes of principal neurons are distributed in precise ratios and at specific positions in these layers and are generated by the same neural progenitor cells (NPCs), steered by a spatially and temporally specified combination of molecular cues that are incompletely understood. Recently, we discovered that an alternatively spliced isoform of the TrkC receptor lacking the kinase domain, TrkC-T1, is a determinant of the corticofugal projection neuron (CFuPN) fate. Here, we show that the finely tuned balance between TrkC-T1 and the better known, kinase domain-containing isoform, TrkC-TK+, is cell type-specific in the developing cortex and established through the antagonistic actions of two RNA-binding proteins, Srsf1 and Elavl1. Moreover, our data show that Srsf1 promotes the CFuPN fate and Elavl1 promotes the callosal projection neuron (CPN) fate in vivo via regulating the distinct ratios of TrkC-T1 to TrkC-TK+. Taken together, we connect spatio-temporal expression of Srsf1 and Elavl1 in the developing neocortex with the regulation of TrkC alternative splicing and transcript stability and neuronal fate choice, thus adding to the mechanistic and functional understanding of alternative splicing in vivo

Ire1α-Regulated Rate of mRNA Translation is Required for Acquisition of Identity and Polarity in Upper Layer Cortical Neurons

Evolutionary expansion of the neocortex is associated with the increase in upper layer neurons. Here, we present Inositol-Requiring Enzyme 1α, Ire1α, as an essential determinant of upper layer fate, neuronal polarization and cortical lamination. We demonstrate a non-canonical function of Ire1α in the regulation of global translation rates in the developing neocortex through its dynamic interaction with the ribosome and regulation of eIF4A1 and eEF-2 expression. Inactivation of Ire1α engenders lower protein synthesis rates associated with stalled ribosomes and decreased number of translation start sites. We show unique sensitivity of upper layer fate to translation rates. Whereas eEF-2 is required for cortical lamination, eIF4A1 regulates acquisition of upper layer fate downstream of Ire1α in a mechanism of translational control dependent on 5’UTR-embedded structural elements in fate determinant genes. Our data unveil developmental regulation of ribosome dynamics as post-transcriptional mechanisms orchestrating neuronal diversity establishment and assembly of cortical layers

Protein translation rate determines neocortical neuron fate

The mammalian neocortex comprises an enormous diversity regarding cell types, morphology, and connectivity. In this work, we discover a post-transcriptional mechanism of gene expression regulation, protein translation, as a determinant of cortical neuron identity. We find specific upregulation of protein synthesis in the progenitors of later-born neurons and show that translation rates and concomitantly protein half-lives are inherent features of cortical neuron subtypes. In a small molecule screening, we identify Ire1α as a regulator of Satb2 expression and neuronal polarity. In the developing brain, Ire1α regulates global translation rates, coordinates ribosome traffic, and the expression of eIF4A1. Furthermore, we demonstrate that the Satb2 mRNA translation requires eIF4A1 helicase activity towards its 5’-untranslated region. Altogether, we show that cortical neuron diversity is generated by mechanisms operating beyond gene transcription, with Ire1α-safeguarded proteostasis serving as an essential regulator of brain development

Prolonged transfer of feces from the lean mice modulates gut microbiota in obese mice

BACKGROUND: Transplanting a fecal sample from lean, healthy donors to obese recipients has been shown to improve metabolic syndrome symptoms. We therefore examined the gut microbiota in mice after administering a long-term, high-fat diet (HFD) supplemented with feces from lean mice through the fecal-oral route. METHODS: C57BL6/W mice were allowed to adapt to a non-specific pathogen free (SFP) environment for 2 weeks before being divided into three groups of 16 animals. Animals were fed for 28 weeks with a normal diet (ND), HFD or HFD supplemented with feces from ND-fed mice (HFDS). The composition of colonizing bacteria was evaluated in droppings collected under SPF conditions at the beginning of the study and at 12 and 28 weeks using an 16S Metagenomics Kit on Ion PGM sequencer. RESULTS: HFD and HFDS-fed mice attained (p < 0.05) greater body weights by weeks 6 and 5, respectively. HFDS-fed mice gained more weight than HFD-fed mice by week 25. Both species diversity and richness indices increased with time in HFDS mice only. CONCLUSIONS: Prolonged HFD-fed mice supplementation with feces from lean mice altered bacteria species diversity and richness, accelerated the onset of obesity, and caused increased weight gain in the later weeks of the HFD regimen. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12986-016-0116-8) contains supplementary material, which is available to authorized users