Peripheral calcifying cystic odontogenic tumour of the maxillary gingiva

BACKGROUND: Odontogenic tumors are lesions that are derived from remnants of the components of the developing tooth germ. The calcifying cystic odontogenic tumor or calcifying odontogenic cyst is a benign cystic neoplasm of odontogenic origin that is characterized by an ameloblastoma-like epithelium and ghost cells. Calcifying cystic odontogenic tumor may be centrally or peripherally located, and its ghost cells may exhibit calcification, as first described by Gorlin in 1962. Most peripheral calcifying cystic odontogenic tumors are located in the anterior gingiva of the mandible or maxilla. CASE PRESENTATION: Authors report a rare case of a peripheral calcifying cystic odontogenic tumor of the maxillary gingiva. A 39-year-old male patient presented with a fibrous mass on the attached buccal gingiva of the upper left cuspid teeth. It was 0.7-cm-diameter, painless and it was clinically diagnosed as a peripheral ossifying fibroma. After an excisional biopsy, the diagnosis was peripheric calcifying cystic odontogenic tumor. The patient was monitored for five years following the excision, and no recurrence was detected. CONCLUSIONS: All biopsy material must be sent for histological examination. If the histological examination of gingival lesions with innocuous appearance is not performed, the frequency of peripheral calcifying cystic odontogenic tumor and other peripheral odontogenic tumors may be underestimated

Effects of Chamomilla recutita(L.) on oral wound healing in rats

Objectives. The purpose of this study was to evaluate the effect of Chamomilla recutita on the healing of ulcers in rats. Study Design. A 5-mm wound was inflicted on the tongue of 36 rats. Treatment group animals were treated topically with 0.04 mL/day of chamomile ointment, whereas control group animals were not treated. Animals were sacrificed after 3, 7 or 10 days. Semi-quantitative analysis of the degree of inflammation, fibroblast count and wound size was performed, as well as histometric analysis of re-epithelialization and percentage of collagen fibers of the lesion. Results. Animals treated with chamomile showed the best results regarding epithelialization and percentage of collagen fibers after 10 days. As expected, time had a statistically significant effect(p< 0.05) on fibroblast count, epithelialization, inflammation and wound size; animals sacrificed at 3 days showed the worst results. Conclusions. Chamomile stimulated re-epithelialization and the formation of collagen fibers after 10 days of treatment; it did not, however, influence inflammation or fibroblast count. © Medicina Oral S. L

Vascular leiomyoma in the oral cavity : report of two cases

Vascular leiomyomas (VL) are benign lesions of perivascular origin. We report two new cases and discuss their clinical, histological and immunohistochemical characteristics, in order to facilitate the diagnosis and treatment of such lesions. The patients, both male, presented asymptomatic nodules located in the bottom of the labial sulcus and buccal mucosa. In the second case, color doppler ultrasonography was performed, which showed no change in blood flow. After excisional biopsies, a limited lesion was observed histologically, with multiple tortuous vessels of varying sizes and calibers, and among them, spindle cells bundles, positive for smooth muscle actin. Oral VLs have clinical features similar to those of other more common lesions, making preoperative diagnosis difficult. Imaging examination, such as color doppler ultrasonography, may help in diagnosis. In general, excisional biopsy is performed, due to the ease of removal of the lesion during surgery. The treatment of choice is the complete excision of the lesion, which has an excellent prognosis and a low rate of recurrence

Spindle Cell Lipoma Occurring in the Buccal Mucosa: An Unusual Location of This Benign Lipomatous Neoplasm

Spindle cell lipoma is a benign lipomatous neoplasm, which rarely occurs in the oral cavity. The aims of this paper are to report a case of spindle cell lipoma located in buccal mucosa and discuss the main clinical, histological, and immunohistochemical findings of this entity. Thus, we report a 4-year history of an asymptomatic smooth surface nodule in an elderly Caucasian man with clinical hypothesis of fibroma. The histopathological examination showed spindle cells, mature adipose tissue, and many mast cells in a stroma of connective tissue presenting ropey collagen fibers bundles. After immunohistochemical analysis, the final diagnosis was spindle cell lipoma.Departamento de Biociências e Diagnóstico Bucal, Instituto de Ciência e Tecnologia (ICT), Universidade Estadual Paulista (UNESP), São José dos Campos, SP, BrasilUniversidade Estadual Paulista, Departamento de Biociências e Diagnóstico Bucal, Instituto de Ciência e Tecnologia de São José dos Campo

PERFIL EPIDEMIOLÓGICO, TRATAMENTO E SOBREVIDA DE PACIENTES COM CÂNCER BUCAL EM TAUBATÉ E REGIÃO

O propósito deste trabalho foi delinear o perfil epidemiológico dos pacientes com câncer bucal atendidos de 1993 a 1998, na Clínica de Diagnóstico Bucal do Departamento de Odontologia da UNITAU, além de avaliar dados relacionados ao estadiamento clínico, tratamento proposto e sobrevida. Foi realizada análise retrospectiva dos prontuários de 35 pacientes com diagnóstico de câncer bucal, considerando-se as variáveis gênero, idade, raça, procedência, hábitos, localização, tempo de evolução e diagnóstico histológico da lesão, estadiamento da doença, tipo de tratamento e sobrevida do paciente. Foi utilizado o teste exato de Fisher para testar a dependência entre a variável sexo e o restante dos dados avaliados (

Endodontic cements: morphological analysis carried out immediately and after a six-month storage, using atomic force microscopy

The aim of this study was to analyze the high resolution morphological characteristics of Sealapex, Apexit, Sealer 26 (calcium hydroxide cements) and Ketac Endo (glass ionomer cement), using atomic force microscopy (AFM), immediately after root canal obturation and six months after it, keeping the roots stored in human blood plasma. The AFM evaluation used 16 single-rooted human teeth embedded in resin blocks after biomechanical preparation. The samples were divided into four groups (four roots each) and the passive lateral condensation filling technique was used in the obturation of the canals with the mentioned sealers. Apexit suffered the highest degree of disintegration after the six-month storage in human blood plasma, followed by Ketac Endo and Sealapex. Sealer 26 was the most uniform cement and suffered the least disintegration.O objetivo deste trabalho foi analisar a morfologia dos cimentos Sealapex, Apexit, Sealer 26 (cimentos a base de hidróxido de cálcio) e Ketac Endo (cimento de ionômero de vidro), através da microscopia de força atômica, verificando-se as características de suas partículas após a obturação dos canais radiculares e após um período de seis meses de contato com o plasma sanguíneo humano. Utilizaram-se 16 dentes unirradiculares humanos extraídos e incluídos em blocos de resina após o preparo biomecânico. As raízes foram divididas em quatro grupos de quatro raízes cada e os canais radiculares obturados pela técnica de condensação lateral passiva com os cimentos em estudo. Verificou-se que o cimento Apexit foi o que mais sofreu desintegração após seis meses de imersão em plasma sanguíneo humano, seguido pelo Ketac Endo e Sealapex. Dentre todos os cimentos estudados, o Sealer 26 mostrou-se o mais uniforme e com a menor desintegração.19920

Efeitos do laser de baixa potência, sob diferentes protocolos, no reparo de feridas cutâneas em ratos

Although there are many studies confirming laser biomodulation, appropriate parameters and treatment protocols have not yet been established. The aim of this study was to analyze the effects of a low-level laser therapy under different protocols on skin wound healing in rats. Standardized 8-mm circular wounds were made in the dorsal midline backs of forty rats. The animals were divided into four groups: G1, without laser; G2, laser immediately after surgery; G3, laser immediately after surgery and again after 48 h; G4, laser immediately after surgery, after 48 h and after seven days. The wound areas were measured immediately after surgery, after 48 h, after seven days and after ten days (when the animals were sacrificed) using an image analysis software. After sacrifice, the wounds and surrounding tissues were removed and submitted to routine laboratorial processing and histological slide confection. Six slides of each animal were photographed, and fibroblast counts were made. For statistical evaluation, the Kruskal-Wallis test and a 5% significance level were used. The control group showed less progression of repair than other groups at all observation times, highlighting the effect of lasers in healing in biometrical analysis. Irradiated groups showed increased fibroblast proliferation relative to the control group, and the protocol with two periods of irradiation resulted in higher fibroblast numbers after ten days. It was concluded that the laser treatment aided in wound healing and that the repair process was faster in the groups that received more than one application of the laser.Apesar dos numerosos trabalhos confirmando o potencial biomodulador do laser, os parâmetros e protocolos ideais para sua aplicação ainda não foram bem estabelecidos. O objetivo deste trabalho foi analisar os efeitos do laser de baixa potência, sob diferentes protocolos, na reparação de feridas cutâneas em ratos. Depois de realizadas feridas padronizadas de 8 mm de diâmetro no centro do plano sagital médio no dorso de quarenta ratos, os animais foram divididos em quatro grupos: G1, sem exposição ao laser; G2, exposição ao laser imediatamente após a cirurgia; G3, exposição ao laser conforme G2 e 48h após cirurgia; G4, exposição ao laser idem a G3 e sete dias após a cirurgia. As áreas das feridas foram medidas imediatamente após cirurgia, 48h, sete dias e no décimo dia (sacrifício) utilizando-se programa para análise de imagens. Após o sacrifício, removeram-se as áreas correspondentes às feridas e o tecido ao redor e procedeu-se ao preparo de rotina para confecção de lâminas histológicas. Seis cortes de cada animal foram fotografados e realizou-se contagem de fibroblastos. Para a análise estatística, utilizou-se o teste de Kruskal-Wallis (5%). Em todos os períodos, G1 apresentou a menor área de reparação, evidenciando os efeitos do laser na redução macroscópica das lesões. Os grupos irradiados apresentaram também proliferação de fibroblastos superior ao controle, sendo que o protocolo de duas aplicações resultou no maior número de fibroblastos aos dez dias. Concluiu-se que o laser favoreceu a cicatrização e que o processo foi acelerado nos grupos que receberam mais de uma aplicação

Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model

Periodontal research involves the use of animal models to better understand the biological processes of periodontal diseases and the potential of new or existing therapies. Currently, ligature-induced periodontitis in rats is the main model used in periodontal research, in this model, alveolar bone loss (ABL) is the main parameter evaluated by radiographic, morphometric, and histological techniques. Interestingly, although these methodologies are widely used, it is not totally clarified neither the kinetics of ABL over the induction time nor the agreement degree (repeatability and reproducibility) of these techniques. Objective: To characterize ABL kinetics at 0, 3, 7, 15, 30, and 60 days after ABL induction by ligature and to evaluate the intra- (repeatability) and inter-examiner (reproducibility) agreement and the correlation among the radiographic, morphometric, and histological methodologies. Material and Methods: 60 male Wistar rats with induced ABL were randomly divided into 6 experimental groups (n = 10 animals/group). After 0, 3, 7, 15, 30, and 60 days, the animals were euthanized and their hemimandibles were removed for ABL determination using radiographic, morphometric and histological techniques. Results: Radiographic and morphometric/linear techniques allowed the detection of statistically significant ABL on the third day, while histological and morphometric/area techniques could only detect ABL after the seventh day (ANOVA/Tukey, p;0.9,