Perfil epidemiológico das uroculturas positivas no Hospital Universitário durante janeiro-dezembro de 2001.

Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Departamento de Saúde Pública, Curso de Medicina, Florianópolis, 200

Automation of a cell culture process with lager vessels

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Appraisal of the mixing performance of Single-Use shaken bioreactors

The pharmaceutical industry is at the forefront of the production of antibodies using mammalian cell-based cultures, with single-use technologies gaining prominence in the manufacturing process. At laboratory scale mammalian cells are usually grown in low shear devices, with disposable shaken bioreactors being largely employed in the early stages of bioprocess development. It has been a recent industry trend to use shaken bioreactors at large scale in the upstream process, performing cell culture in single-use bags which eliminate the need for cleaning in place, offer flexibility and lower production down-times. Single-use Orbitally Shaken Bioreactors (OSRs) consist of a shaker, a structural support to which a disposable bag conforms to and all ancillary connections and controllers. Production scale OSRs with single-use bags, employ the agitation principle of shaken flasks and microwell plates, providing a homogeneously single-use upstream scale-up process thus facilitating scaling-up and simplifying regulatory approval. The aim of the work is to characterize the mixing and flow dynamics in a cylindrical orbitally shaken bioreactor with conical bottoms of different heights. The rationale for a conical bottom is to ease the suspension of cells or microcarriers, which are used for the cultivation of cells not yet adapted for suspend culture, such as legacy cell lines and stem cells. The geometry of the conical bottom was designed so to be compatible with single-use bags, as the conical bottom is truncated and the cone obtuse. This study builds upon previous works of the research group (Weheliye et al 2013, Rodriguez et al. 2013, Rodriguez et .al. 2014, Ducci and Weheliye, 2014) for flat bottom reactors, where increases in Froude number were found to determine a mean flow transition and to increase the turbulence levels. The major objective of the current work is to determine the performances of shaken bioreactors with conical bottom, and to assess to what extent the mean flow and flow regime transitions already identified for a flat bottom are affected by the geometry variation. Particle Image Velocimetry, PIV, and Dual Indicator System for Mixing Time, DISMT, were employed to assess the mixing performances in the bioreactor with a conical bottom. DISMT (see Rodriguez et al., 2014) consists in a colorimetric method where two pH indicators are used to visualise the level of mixing reached after insertion of an acid solution. The findings of the current study provides insight into the flow of a single-use shaken bioreactor and offers a novel approach to design the next generation of products and improve scaling methodologies

Cada mergulho é um flash?

O presente texto objetiva proporcionar uma reflexão sobre o compartilhamento indevido de imagens que retratam cenas de violência e/ou outros acontecimentos nefastos da sociedade para fins de engajamento nas redes sociais.&nbsp

Orbital shaken bioreactor for influenza A virus production in high cell density cultivations

The majority of large scale cell culture processes is performed in stirred tank bioreactors (STR) and process development employs scale down models of the same bioreactor type. However, for screening of clones or process conditions at even smaller scale, shake flasks (SF) represent the most widely used model. Occasionally, SF allow for robust processes that cannot be transferred to STR because sensitive cells cannot cope with mechanical stress in STR due to stirring and aeration. Orbital shaken bioreactors (OSB) are a valuable alternative to STR as the transfer from SF to OSB is simplified because the systems rely on the same basic principles for mixing and aeration (e.g., bubble-free surface gassing). In particular, high oxygen transfer rates and short mixing times combined with low shear stress can also be achieved in OSB. These benefits may be even more pronounced in high density culture processes. Please click Additional Files below to see the full abstract

Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors

Background Conventional experiments in small scale are often performed in a Black Box fashion, analyzing only the product concentration in the final sample. Online monitoring of relevant process characteristics and parameters such as substrate limitation, product inhibition and oxygen supply is lacking. Therefore, fully equipped laboratory-scale stirred tank bioreactors are hitherto required for detailed studies of new microbial systems. However, they are too spacious, laborious and expensive to be operated in larger number in parallel. Thus, the aim of this study is to present a new experimental approach to obtain dense quantitative process information by parallel use of two small-scale culture systems with online monitoring capabilities: Respiration Activity MOnitoring System (RAMOS) and the BioLector device. Results The same mastermix (medium plus microorganisms) was distributed to the different small-scale culture systems: 1) RAMOS device; 2) 48-well microtiter plate for BioLector device; and 3) separate shake flasks or microtiter plates for offline sampling. By adjusting the same maximum oxygen transfer capacity (OTRmax), the results from the RAMOS and BioLector online monitoring systems supplemented each other very well for all studied microbial systems (E. coli, G. oxydans, K. lactis) and culture conditions (oxygen limitation, diauxic growth, auto-induction, buffer effects). Conclusions The parallel use of RAMOS and BioLector devices is a suitable and fast approach to gain comprehensive quantitative data about growth and production behavior of the evaluated microorganisms. These acquired data largely reduce the necessary number of experiments in laboratory-scale stirred tank bioreactors for basic process development. Thus, much more quantitative information is obtained in parallel in shorter time.Cluster of Excellence “Tailor-Made Fuels from Biomass”, which is funded by the Excellence Initiative by the German federal and state governments to promote science and research at German universities

Producing viruses in orbit: Current developments for orbital shaken viral vaccine manufacturing

Preculture of suspension cells is successfully performed in shake flasks. Especially newly developed designer cells are passaged up to 100 times in shake flaks at high shaking frequency and are then perfectly adapted to growth in a CO2 incubator with pH control and maximum oxygen supply (typically above 80% pO2). When they are subsequently transferred to stirred tank bioreactors for scaling up, specific cell growth rates are often lower and cells become sensitive to pH control via acid/base addition and shear stress due to submers gassing (bubbles). This was also seen for avian AGE1.CR.pIX and human HEK 293 cells. To avoid these problems, scale up in shaken mode was evaluated. Here we present the latest developments of the SB10-X OSB bioreactor with regard to bag design and improvement of the control unit. A new control strategy was introduced leading to a faster and more precise pH and DO control. Furthermore, the perfusion bag was optimized, so that on TFF or two ATF systems can be easily connected. Both developments have led to a more robust SB10-X system that allows to easily perform batch, fed batch or perfusion runs. Please click Download on the upper right corner to see the full abstract