Comparing Canadian and U.S. Cattle Feeding Pricing Practices and Perceptions of Pricing Issues

Competition among beef packing firms, use of so-called captive supply, and methods of price discovery have been prolonged, contentious issues in the U.S. beef industry for two decades or more. While of lesser apparent concern in Canada for many years, these same matters rose to the forefront of beef industry issues after the Canada–U.S. border closure that resulted from Canada’s first case of bovine spongiform encephalopathy (BSE) in May 2003.Marketing,

The Determination Of Reddening From Intrinsic VR Colors Of RR Lyrae Stars

New R-band observations of 21 local field RR Lyrae variable stars are used to explore the reliability of minimum light (V-R) colors as a tool for measuring interstellar reddening. For each star, R-band intensity mean magnitudes and light amplitudes are presented. Corresponding V-band light curves from the literature are supplemented with the new photometry, and (V-R) colors at minimum light are determined for a subset of these stars as well as for other stars in the literature. Two different definitions of minimum light color are examined, one which uses a Fourier decomposition to the V and R light curves to find (V-R) at minimum V-band light, (V-R)_{min}^F, and the other which uses the average color between the phase interval 0.5-0.8, (V-R)_{min}^{\phi(0.5-0.8)}. From 31 stars with a wide range of metallicities and pulsation periods, the mean dereddened RR Lyrae color at minimum light is (V-R)_{min,0}^F = 0.28 pm 0.02 mag and (V-R)_{min,0}^{\phi(0.5-0.8)} = 0.27 pm 0.02 mag. As was found by Guldenschuh et al. (2005) using (V-I) colors, any dependence of the star's minimum light color on metallicity or pulsation amplitude is too weak to be formally detected. We find that the intrinsic (V-R) of Galactic bulge RR Lyrae stars are similar to those found by their local counterparts and hence that Bulge RR0 Lyrae stars do not have anomalous colors as compared to the local RR Lyrae stars.Comment: accepted by A

An Oosterhoff Analysis of the Galactic Bulge Field RR Lyrae stars: Implications On Their Absolute Magnitudes

We present an analysis of the period-$V$-amplitude plane for RR0 Lyrae stars (fundamental mode pulsators) with "normal" light curves in the bulge using the MACHO bulge fields. Although bulge globular clusters have RR Lyraes that divide into two reasonable distinct groups according to the average period of the RR0 Lyraes (Oosterhoff 1939), there is no evidence of a gap between Oosterhoff I and II stars in the bulge field star sample. The majority of the bulge RR0 Lyrae field star population have a difference in period compared to the Oosterhoff I cluster M3 (Delta log P) that is shifted by about 0.02 days with regard to the Milky Way Oosterhoff I population, and the sample includes stars with Delta log P > 0.06 days, a characteristic hardly seen in Milky Way globular clusters. The metal-rich RR0 Lyrae stars in the Galactic bulge sample have Delta log P values on the other side of the spectrum as those in the metal-rich globular clusters NGC 6388 and NGC 6441. We find that the $V$-amplitude for a given period is a function of Delta log P, and not of metal abundance, similar to the result found by Clement & Shelton (1999) for RR Lyrae stars in Milky Way globular clusters. A comparative study of the bulge field stars with similar metallicities but different Oosterhoff types is carried out. Bulge field RR0 Lyrae variables with Delta log P values similar to Oosterhoff II clusters are about 0.2 mag brighter than RR0 Lyrae variables with Delta log P similar to Oosterhoff I clusters. Reliance upon a single absolute magnitude-[Fe/H] relation may be inappropriate when considering populations with different Delta log P.Comment: accepted to AJ, 9 figure

Diagnostic applications of next generation sequencing: working towards quality standards

Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major NGS technology companies providing commercially available instruments (Roche 454, Illumina, Life Technologies) have recently marketed bench top sequencing instruments with lower throughput and shorter run times, thereby broadening the applications of NGS and opening the technology to the potential use for clinical diagnostics. Although the high expectations regarding the discovery of new diagnostic targets and an overall reduction of cost have been achieved, technological challenges in instrument handling, robustness of the chemistry and data analysis need to be overcome. To facilitate the implementation of NGS as a routine method in molecular diagnostics, consistent quality standards need to be developed. Here the authors give an overview of the current standards in protocols and workflows and discuss possible approaches to define quality criteria for NGS in molecular genetic diagnostics

Identification of human viral protein-derived ligands recognized by individual MHCI-restricted T-cell receptors

Evidence indicates that autoimmunity can be triggered by virus-specific CD8+ T cells that crossreact with self-derived peptide epitopes presented on the cell surface by major histocompatibility complex class I (MHCI) molecules. Identification of the associated viral pathogens is challenging because individual T-cell receptors can potentially recognize up to a million different peptides. Here, we generate peptide length-matched combinatorial peptide library (CPL) scan data for a panel of virus-specific CD8+ T-cell clones spanning different restriction elements and a range of epitope lengths. CPL scan data drove a protein database search limited to viruses that infect humans. Peptide sequences were ranked in order of likelihood of recognition. For all anti-viral CD8+ T-cell clones examined in this study, the index peptide was either the top-ranked sequence or ranked as one of the most likely sequences to be recognized. Thus, we demonstrate that anti-viral CD8+ T-cell clones are highly focused on their index peptide sequence and that ‘CPL-driven database searching’ can be used to identify the inciting virus-derived epitope for a given CD8+ T-cell clone. Moreover, to augment access to CPL-driven database searching, we have created a publicly accessible webtool. Application of these methodologies in the clinical setting may clarify the role of viral pathogens in the etiology of autoimmune diseases

Experiences of using the Theoretical Domains Framework across diverse clinical environments: a qualitative study

This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.phpBACKGROUND: The Theoretical Domains Framework (TDF) is an integrative framework developed from a synthesis of psychological theories as a vehicle to help apply theoretical approaches to interventions aimed at behavior change. PURPOSE: This study explores experiences of TDF use by professionals from multiple disciplines across diverse clinical settings. METHODS: Mixed methods were used to examine experiences, attitudes, and perspectives of health professionals in using the TDF in health care implementation projects. Individual interviews were conducted with ten health care professionals from six disciplines who used the TDF in implementation projects. Deductive content and thematic analysis were used. RESULTS: Three main themes and associated subthemes were identified including: 1) reasons for use of the TDF (increased confidence, broader perspective, and theoretical underpinnings); 2) challenges using the TDF (time and resources, operationalization of the TDF) and; 3) future use of the TDF. CONCLUSION: The TDF provided a useful, flexible framework for a diverse group of health professionals working across different clinical settings for the assessment of barriers and targeting resources to influence behavior change for implementation projects. The development of practical tools and training or support is likely to aid the utility of TDF