Molecular weight cut off (MWCO) determination in ultra- and nanofiltration: Review of methods and implications on organic matter removal

Estimation of membrane molecular weight cut off (MWCO) in the range between ultrafiltration (UF) and nanofiltration (NF) is challenging because retention is not controlled only by size exclusion. This review provides an experimental and theoretical overview of the membrane MWCO in the range from UF to loose NF (from 500 to 0.7 kDa) to evaluate the significance of membrane MWCO on predicting retention of organic solutes when approaching NF pore structure. The experimental section includes filtration of: i) organic tracers with different molecular weights (MW) and properties, such as polyethylene glycol (PEG) and oligosaccharides, and ii) natural organic matter (e.g. humic acid, alginic acid, Tanzanian and Australian organic matter) in the MWCO range between UF and NF, at minimal concentration polarization. The role of molecule structure, size exclusion and charge shielding when filtering organic solutes is elucidated. The molecular structure of uncharged organic tracers plays a major role on MWCO estimation, especially for loose NF membranes, where oligosaccharides are retained more effectively compared to PEG tracers of similar MW. The MWCO determined by PEG filtration and estimated from the pore radius distribution are consistent in the UF range from 1 to 500 kDa, indicating major contribution of size exclusion. Conversely, MWCO of loose NF membranes determined with PEG tracers is overestimated. Charged organics, such as humic acid (1.5 kDa < MW < 3 kDa), shows retention between 60 and 80 % for UF membrane MWCO below 30 kDa (pore radius < 14 nm) and full retention by loose NF (pore radius below 1.4 nm). This is explained with an interplay of size exclusion and charge shielding in the pore. This review can assist in the selection of the organic tracer and operating conditions for membrane MWCO determination between UF and NF, elucidating the relevance of membrane MWCO in organic matter retention

Functionalized composite nanofiber membranes for selective steroid hormone micropollutants uptake from water: Role of cyclodextrin type

Cyclodextrins (CD) entrapped in nanofiber composite membranes are potential selective adsorbing materials to remove steroid hormone (SHs) micropollutants from water. This study aims to elucidate the role of CD macrocyclic host type on the SHs inclusion complexation and uptake in filtration. Three CD types (α, β, and γ) are cross-linked with epichlorohydrin to form polymers (αCDP, βCDP and γCDP) and entrapped into a nanofiber composite membrane by electrospinning. TGA analysis confirmed the CD entrapment into the nanofiber without loss of CD molecules during filtration. The CD type plays a dominant role in controlling the removal of different SHs. A similar removal (range 33 to 50 %) was observed with αCDP, irrespective of the SH type. In contrast, removal and uptake dependent on SH type were observed for β and γCDP, with the highest removal of 74 % for progesterone, followed by estradiol (46 %) and estrone (27 %) and the lowest removal of 3 % for testosterone. Molecular dynamic (MD) simulation revealed a stronger and more stable complex formed with βCDP, as demonstrated by: i) the closer spatial distribution of SH molecules from the βCDP cavity and, ii) the quantum chemistry calculations of the lower de-solvation energy (+6.0 kcal/mol), which facilitates the release of water molecules from interacting interface of CD molecule and hormone. Regarding γCDP, the highest de-solvation energy (+8.3 kcal/mol) poses an energetic barrier, which hinders the formation of the inclusion complex. In the case of αCDP, a higher interaction energy (-8.9 kcal/mol) compared to βCDP (-4.9 kcal/mol) was obtained, despite the broader spatial distribution observed from the MD simulation attributed to a dominant hydrogen bonding interaction with the OH primary groups on the external surface cavity. The findings highlight the relevance of the CD type in designing selective adsorbing membranes for steroid hormone micropollutant uptake. Experimental results and MD simulation suggest that βCD is the most suitable CD type for steroid hormone uptake, due to a more stable and stronger inclusion complexation than α and γCD

The Madness of Multiple Entries in March Madness

This paper explores multi-entry strategies for betting pools related to single-elimination tournaments. In such betting pools, participants select winners of games, and their respective score is a weighted sum of the number of correct selections. Most betting pools have a top-heavy payoff structure, so the paper focuses on strategies that maximize the expected score of the best-performing entry. There is no known closed-formula expression for the estimation of this metric, so the paper investigates the challenges associated with the estimation and the optimization of multi-entry solutions. We present an exact dynamic programming approach for calculating the maximum expected score of any given fixed solution, which is exponential in the number of entries. We explore the structural properties of the problem to develop several solution techniques. In particular, by extracting insights from the solutions produced by one of our algorithms, we design a simple yet effective problem-specific heuristic that was the best-performing technique in our experiments, which were based on real-world data extracted from recent March Madness tournaments. In particular, our results show that the best 100-entry solution identified by our heuristic had a 2.2% likelihood of winning a $1 million prize in a real-world betting pool

The Orbit of NGC 5907 ULX-1

We report on the orbit of the binary system powering the most extreme ultraluminous X-ray pulsar known to date: NGC 5907 ULX-1 (hereafter ULX1). ULX1 has been the target of a substantial multi-instrument campaign, mainly in the X-ray band, but no clear counterparts are known in other bands. Although ULX1 is highly variable and pulsations can be transient (regardless of the source flux), the timing data collected so far allow us to investigate the orbit of this system. We find an orbital period $P_{orb}=5.7^{+0.1}_{-0.6}\text{ d}$ and a projected semi-axis $A_1 =3.1^{+0.8}_{-0.9}\text{ lts}$. The most likely ephemeris is: $P_{orb}=5.6585(6)\text{ d}$, $A_1 = 3.1(4)\text{ lts}$, and the epoch of ascending nodes passage is: $T_{asc} = 57751.37(5)\text{ MJD}$. However, there are 6 similar solutions, acceptable within $3\,\sigma$. We find further indications that ULX1 is a high-mass X-ray binary. This implies that we are observing its orbit face-on, with an inclination $<5\text{ deg}$.Comment: 11 pages, 2 tables, 6 figure

The Orbit of NGC 5907 ULX-1

© 2024. The Author(s). Published by the American Astronomical Society. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/Wereport on the orbit of the binary system powering the most extreme ultraluminous X-ray pulsar known to date: NGC5907ULX-1(hereafter ULX1). ULX1 has been the target of a substantial multi-instrument campaign, mainly in the X-ray band, but no clear counterparts are known in other bands. Although ULX1 is highly variable and pulsations can be transient (regardless of the source flux), the timing data collected so far allow us to investigate the orbit of this system. We find an orbital period P orb =+ 5.7 0.1 0.6 days and a projected semi-axis 0.8 1 A 3.1 lt s =+. The most likely ephemeris is Porb = 5.6585(6) days, A1 =3.1(4) lt-s, and the epoch of ascending nodes passage is Tasc = 57751.37(5) MJD. However, there are six similar solutions acceptable within 3σ.Wefind further indications that ULX1 is a high-mass X-ray binary. This implies that we are observing its orbit face on, with an inclination <5°.Peer reviewe

In vitro and in vivo single-agent efficacy of checkpoint kinase inhibition in acute lymphoblastic leukemia

Background: Although progress in children, in adults, ALL still carries a dismal outcome. Here, we explored the in vitro and in vivo activity of PF-00477736 (Pfizer), a potent, selective ATP-competitive small-molecule inhibitor of checkpoint kinase 1 (Chk1) and with lower efficacy of checkpoint kinase 2 (Chk2). Methods: The effectiveness of PF-00477736 as single agent in B-/T-ALL was evaluated in vitro and in vivo studies as a single agent. The efficacy of the compound in terms of cytotoxicity, induction of apoptosis, and changes in gene and protein expression was assessed using different B-/T-ALL cell lines. Finally, the action of PF-00477736 was assessed in vivo using leukemic mouse generated by a single administration of the tumorigenic agent n-ethyl-n-nitrosourea. Results: Chk1 and Chk2 are overexpressed concomitant with the presence of genetic damage as suggested by the nuclear labeling for \u3b3-H2A.X (Ser139) in 68 % of ALL patients. In human B-and T-ALL cell lines, inhibition of Chk1/2 as a single treatment strategy efficiently triggered the Chk1-Cdc25-Cdc2 pathway resulting in a dose-and time-dependent cytotoxicity, induction of apoptosis, and increased DNA damage. Moreover, treatment with PF-00477736 showed efficacy ex vivo in primary leukemic blasts separated from 14 adult ALL patients and in vivo in mice transplanted with T-ALL, arguing in favor of its future clinical evaluation in leukemia. Conclusions: In vitro, ex vivo, and in vivo results support the inhibition of Chk1 as a new therapeutic strategy in acute lymphoblastic leukemia, and they provide a strong rationale for its future clinical investigation

Pancreatic Ductal Adenocarcinoma Associated with Autoimmune Pancreatitis

Autoimmune pancreatitis (AIP), in contrast to other benign chronic pancreatic diseases, can be cured with immunosuppressant drugs, thus the differentiation of AIP from pancreatic cancer is of particular interest in clinical practice. There is the possibility that some patients with AIP may develop pancreatic cancer, and this possibility contributes to increasing our difficulties in differentiating AIP from pancreatic cancer. We herein report the case of a 70-year-old man in whom pancreatic adenocarcinoma and AIP were detected simultaneously. We must carefully monitor AIP patients for the simultaneous presence of pancreatic cancer, even when a diagnosis of AIP is confirmed