The astacin metalloprotease moulting enzyme NAS-36 is required for normal cuticle ecdysis in free-living and parasitic nematodes

Nematodes represent one of the most abundant and species-rich groups of animals on the planet, with parasitic species causing chronic, debilitating infections in both livestock and humans worldwide. The prevalence and success of the nematodes is a direct consequence of the exceptionally protective properties of their cuticle. The synthesis of this cuticle is a complex multi-step process, which is repeated 4 times from hatchling to adult and has been investigated in detail in the free-living nematode, Caenorhabditis elegans. This process is known as moulting and involves numerous enzymes in the synthesis and degradation of the collagenous matrix. The nas-36 and nas-37 genes in C. elegans encode functionally conserved enzymes of the astacin metalloprotease family which, when mutated, result in a phenotype associated with the late-stage moulting defects, namely the inability to remove the preceding cuticle. Extensive genome searches in the gastrointestinal nematode of sheep, Haemonchus contortus, and in the filarial nematode of humans, Brugia malayi, identified NAS-36 but not NAS-37 homologues. Significantly, the nas-36 gene from B. malayi could successfully complement the moult defects associated with C. elegans nas-36, nas-37 and nas-36/nas-37 double mutants, suggesting a conserved function for NAS-36 between these diverse nematode species. This conservation between species was further indicated when the recombinant enzymes demonstrated a similar range of inhibitable metalloprotease activities

A strong shallow heat source in the accreting neutron star MAXI J0556-332

An accretion outburst in an X-ray transient deposits material onto the neutron star primary; this accumulation of matter induces reactions in the neutron star's crust. During the accretion outburst these reactions heat the crust out of thermal equilibrium with the core. When accretion halts, the crust cools to its long-term equilibrium temperature on observable timescales. Here we examine the accreting neutron star transient MAXI J0556-332, which is the hottest transient, at the start of quiescence, observed to date. Models of the quiescent light curve require a large deposition of heat in the shallow outer crust from an unknown source. The additional heat injected is $\approx 4\textrm{-}10\,\mathrm{MeV}$ per accreted nucleon; when the observed decline in accretion rate at the end of the outburst is accounted for, the required heating increases to $\approx 6\textrm{-}16\,\mathrm{MeV}$. This shallow heating is still required to fit the lightcurve even after taking into account a second accretion episode, uncertainties in distance, and different surface gravities. The amount of shallow heating is larger than that inferred for other neutron star transients and is larger than can be supplied by nuclear reactions or compositionally driven convection; but it is consistent with stored mechanical energy in the accretion disk. The high crust temperature ($T_b \gtrsim 10^{9} \, {\rm K}$) makes its cooling behavior in quiescence largely independent of the crust composition and envelope properties, so that future observations will probe the gravity of the source. Fits to the lightcurve disfavor the presence of Urca cooling pairs in the crust.Comment: 6 pages, 4 figures, submitted to ApJ

Stellar Superfluids

Neutron stars provide a fertile environment for exploring superfluidity under extreme conditions. It is not surprising that Cooper pairing occurs in dense matter since nucleon pairing is observed in nuclei as energy differences between even-even and odd-even nuclei. Since superfluids and superconductors in neutron stars profoundly affect neutrino emissivities and specific heats, their presence can be observed in the thermal evolution of neutron stars. An ever-growing number of cooling neutron stars, now amounting to 13 thermal sources, and several additional objects from which upper limits to temperatures can be ascertained, can now be used to discriminate among theoretical scenarios and even to dramatically restrict properties of nucleon pairing at high densities. In addition, observations of pulsars, including their spin-downs and glitch histories, additionally support the conjecture that superfluidity and superconductivity are ubiquitous within, and important to our understanding of, neutron stars.Comment: 88 pages, 35 figures. Some new references added. To be published in the book "Novel Superfluids", Eds. K. H. Bennemann and J. B. Ketterson (Oxford University Press

Biosynthesis and enzymology of the Caenorhabditis elegans cuticle: identification and characterization of a novel serine protease inhibitor.

The nematode Caenorhabditis elegans represents an excellent model in which to examine nematode gene expression and function. A completed genome, straightforward transgenesis, available mutants and practical genome-wide RNAi approaches provide an invaluable toolkit in the characterization of nematode genes. We have performed a targeted RNAi screen in an attempt to identify components of the cuticle collagen biosynthetic pathway. Collagen biosynthesis and cuticle assembly are multi-step processes that involve numerous key enzymes involved in post-translational modification, trimer folding, procollagen processing and subsequent cross-linking stages. Many of these steps, the modifications and the enzymes are unique to nematodes and may represent attractive targets for the control of parasitic nematodes. A novel serine protease inhibitor was uncovered during our targeted screen, which is involved in collagen maturation, proper cuticle assembly and the moulting process. We have confirmed a link between this inhibitor and the previously uncharacterized bli-5 locus in C. elegans. The mutant phenotype, spatial expression pattern and the over-expression phenotype of the BLI-5 protease inhibitor and their relevance to collagen biosynthesis are discussed

Superbursts from Strange Stars

Recent models of carbon ignition on accreting neutron stars predict superburst ignition depths that are an order of magnitude larger than observed. We explore a possible solution to this problem, that the compact stars in low mass X-ray binaries that have shown superbursts are in fact strange stars with a crust of normal matter. We calculate the properties of superbursts on strange stars, and the resulting constraints on the properties of strange quark matter. We show that the observed ignition conditions exclude fast neutrino emission in the quark core, for example by the direct Urca process, which implies that strange quark matter at stellar densities should be in a color superconducting state. For slow neutrino emission in the quark matter core, we find that reproducing superburst properties requires a definite relation between three poorly constrained properties of strange quark matter: its thermal conductivity, its slow neutrino emissivity and the energy released by converting a nucleon into strange quark matter.Comment: 4 pages, submitted to Ap. J. Let

Iran, America and Iranian American Community in Firoozeh Jazayeri Dumas\u27 Funny in Farsi

Post 9/11 the United States of America concerns the reconstruction of already demonized identities of Arabs and Middle-eastern cultures. Postcolonial works reside in their rendering a tragic or serious image of Middle Easterners to bring the Western (American) audience into sympathizing with the Middle Eastern ethnicities. Could it be the case that a fundamentally humorous (not derogatory) depiction might contribute to easing such cultural tensions? Firoozeh Jazayeri Dumas\u27 works stand out as critically acclaimed and successful works familiarizing the American audience with the more humane, likeable, sweet and funny aspects of the Iranians and Iranian culture, and the hardships of being an Iranian immigrant and becoming a hybrid individual. This article explores the already-hybridized self and psyche of Firoozeh as an Iranian American. She writes about her mother land and her residence country and comparing the way she has written about them can help readers understand how one can make peace between different parts of her identity

Compiler assisted elliptic curve cryptography

Although cryptographic software implementation is often performed by expert programmers, the range of performance and secu- rity driven options, as well as more mundane software engineering issues, still make it a challenge. The use of domain specific language and com- piler techniques to assist in description and optimisation of cryptographic software is an interesting research challenge. Our results, which focus on Elliptic Curve Cryptography (ECC), show that a suitable language allows description of ECC based software in a manner close to the original mathe- matics; the corresponding compiler allows automatic production of an ex- ecutable whose performance is competitive with that of a hand-optimised implementation. Our work are set within the context of CACE, an ongo- ing EU funded pro ject on this general topic