Structure-function relations in phosphorylcholine-binding mouse myeloma proteins

The binding site interactions between the phosphorylcholine (phosphocholine)-binding mouse myeloma proteins TEPC 15, W3207, McPC 603, MOPC 167, and MOPC 511 and the isotopically substituted hapten phosphoryl-[methyl-13C]choline have been investigated using 13C and 31P nuclear magnetic resonance (NMR) spectroscopy. Each protein exhibits a unique NMR pattern, but extensive similarities in chemical shift parameters upon binding of hapten to immunoglobulin suggest a significant degree of conservation of important hapten-binding site interactions. Moreover, independent binding studies, in conjunction with the NMR data, allow construction of a simple model of the binding sites of these antibodies, analyzed in terms of the relative strength of interaction between hapten and two main subsites. The NMR evidence supports the view that the heavy chains of these proteins dominate in interacting with bound phosphorylcholine; the various subspecificities of these proteins for phosphorylcholine analogues can be accounted for by amino acid changes in the hypervariable regions of the heavy chains

All for One and One for All! Disparity Between Overall Crew’s and Individual Rowers’ Pacing Strategies During Rowing

Purpose: This study examined individual contributions to overall pacing strategy during 2- and 5-km rowing trials in a cox-less-4 boat. Methods: A crew of 4 male rowers performed maximal-effort on-water trials over 2 and 5 km, and power output during every individual stroke was measured for each crew member. Mean overall boat and individual rower stroke power were calculated for each 25% epoch (25% of total strokes taken), and power for each individual epoch was calculated as a percentage of mean power maintained over the entire distance. The coefficient of variation was used to determine stroke-to-stroke and epoch-to-epoch variability for individual rowers and the overall boat. Results: In both trials, the overall pacing strategy consisted of a high power output in the initial 25% that decreased in the middle 50% and increased again in the final 25%. However, individual rower data indicate wide variation in individual power profiles that did not always mimic the overall boat profile. Conclusions: This study demonstrates that overall boat power profiles during 2- and 5-km rowing trials are similar to velocity profiles previously reported for individual ergometry and on-water racing events. However, this over-all profile is achieved despite considerable variation in individual rower profiles. Further research is warranted to determine the mechanisms through which individual contributions to overall pacing strategy are regulated and the effectiveness or oth-erwise of seemingly disparate individual strategies on overall performance

Demographic and lifestyle correlates of school attendance, English and Maths attainment, and the occurrence of behavioural sanctions in British secondary school children

Aims: Performance in school is known to predict a number of important outcomes in later life (e.g. socioeconomic status). For this reason it is considered useful to identify aspects of demography and lifestyle that are associated with low school attendance, low academic attainment, and high occurrences of problem behaviour. Study Design: The current study utilised a cross-sectional design; analyses were performed twice due to the availability of two cross-sections of data from the same sample. Place and Duration of Study: Data from the current study were collected from three secondary schools in Cornwall, UK. The first cross-section was collected in December, 2012, and the second was collected in June, 2013. Methodology: The School Information Management System was used to obtain data relating to demography (sex, school and year group attended, eligibility/ineligibility to receive free school meals, and presence/absence of a special educational needs status) and school performance (attendance, attainment at Key Stage 3/Key Stage 4 English and maths, and occurrence of behavioural sanctions). Lifestyle factors (number of sleep hours, and frequency of exercise participation) were assessed via pen and paper questionnaire. Chi-square, chi-square tests of linear association, and between-subjects t-tests were used to establish whether the school performance outcomes were associated with the demographic and lifestyle variables. These analyses were then followed-up with binary logistic regression, to determine whether the observed effects were independent of one another. Results: Low school performance was consistently associated with male sex, school and year group attended, special educational needs status, eligibility to receive free school meals, low sleep hours, and infrequent exercise participation. In addition, below average school attendance was itself predictive of low English and maths attainment, and of a high occurrence of behavioural sanctions. The majority of effects observed were significant at both the univariate and multivariate levels. Conclusions: The identification of demographic and lifestyle correlates of school performance may be useful for detecting at-risk individuals who might benefit from interventions. If such interventions were to be effective, the associated reductions in future unemployment and criminality could be beneficial to society as a whole

Rate of photosynthetic induction in fluctuating light varies widely among genotypes of wheat.

Crop photosynthesis and yield are limited by slow photosynthetic induction in sunflecks. We quantified variation in induction kinetics across diverse genotypes of wheat for the first time. Following a preliminary study that hinted at wide variation in induction kinetics across 58 genotypes, we grew 10 genotypes with contrasting responses in a controlled environment and quantified induction kinetics of carboxylation capacity (Vcmax) from dynamic A versus ci curves after a shift from low to high light (from 50 µmol m-2 s-1 to 1500 µmol m-2 s-1), in five flag leaves per genotype. Within-genotype median time for 95% induction (t95) of Vcmax varied 1.8-fold, from 5.2 min to 9.5 min. Our simulations suggest that non-instantaneous induction reduces daily net carbon gain by up to 15%, and that breeding to speed up Vcmax induction in the slowest of our 10 genotypes to match that in the fastest genotype could increase daily net carbon gain by up to 3.4%, particularly for leaves in mid-canopy positions (cumulative leaf area index ≤1.5 m2 m-2), those that experience predominantly short-duration sunflecks, and those with high photosynthetic capacities

Tests to determine the distribution of power in the shops of the Mechanical Engineering Department K.S.A.C.

Citation: Pyle, Charles A. Protargol in veterinary surgery. Senior thesis, Kansas State Agricultural College, 1907.Morse Department of Special CollectionsIntroduction: The object of these tests was to determine the distribution of power through the different shops of the mechanical engineering department. The line shafting of these shops is driven by means of electrical motors and the individual machines by belt drives. In order to determine the distribution, the output of the motors at different shop loads had to be obtained. For this purpose, two sets of tests were carried on. In the first set the relation between the input in the motors, or the electrical horse-power, and the output, or the brake horse -power, was determined. In this set of tests readings were taken of the voltmeter, ammeter, and of the scales simultaneously. A zero reading was taken while the motor was standing still. A certain weight was then placed on the scales and the prony brake was tightened till the scales balanced and two or three readings were taken about a half minute apart, at the close of every five minutes. An average of these readings was taken so as to avoid any error which might arise from just one reading. The weight on the scale beam was then increased about one pound and the procedure was repeated. This was continued till the motor was at its maximum test load

Assessing the potential for tertiary nitrification in sub-surface flow constructed wetlands

The challenge of how to maintain or improve wastewater treatment performance without causing an excessive increase in energy or costs is increasingly focussed towards ammonia. On small sewage treatment works, solutions have historically been energy intensive: to divert waste to a larger plant, add a polishing step to the end of the process flow sheet or upgrade and replace upstream processes. Constructed wetlands (CWs) offer a low energy alternative to meet these challenges. This review explores oxygen transfer theory; nitrification performance of existing CW systems, and the key affecting factors to be considered when implementing the technology for tertiary treatment upgrades. Future perspectives include the use of artificial aeration and greater consideration of vertical sub-surface flow systems as they achieve the nitrification capacity in a smaller footprint than horizontal flow systems and, where suitable hydraulics permit, can be operated under very low energy demand