Pluronic® F-68 enhances the nanoparticle-cell interaction

No abstract available

Einfluss der nichtionischen Tenside Tween® 20, Tween® 80 und Pluronic® F-68 auf die Interaktion von Nanopartikeln mit Caco-2 Einzelzellen

Im Rahmen dieser Diplomarbeit wurde untersucht, welchen Einfluss die nichtionischen Tenside Tween® 20, Tween® 80 und Pluronic® F-68 auf die Interaktion von Nanopartikeln mit Caco-2 DSMZ Einzelzellen ausüben. Weiters wurden diese Tenside hinsichtlich ihres zytotoxischen Effekts bzw. ihrer Auswirkung auf die Zellviabilität und Zellproliferation untersucht. Die Interaktionsstudien zeigten, dass im Vergleich zu Tensid-freien Medien ein Gehalt von 0,05 %iger Tween® 20, Tween® 80 oder 2,5 %iger Pluronic® F 68 eine maximale Steigerung der Assoziation von Nanopartikeln mit Caco-2 Zellen um das bis zu 3,5-fache des Leerwertes bewirkt. Ein Tween® 20 Gehalt von > 0,25 % bewirkt jedoch eine deutliche Reduktion des Anteils an viabilen Zellen. Bei Tween® 80 und Pluronic® F-68 hingegen konnte nach einstündiger Inkubation im untersuchten Konzentrationensbereich von 0,00025 - 2,5 % kein nachteiliger Effekt auf die Zellviabilität festgestellt werden. Im Rahmen der Zellviabiliätstests und der Untersuchung der Proliferationsrate von Caco-2 Zellen konnte in Gegenwart von Tween® 20 und Tween® 80 kein positiver Effekt festgestellt werden. Im Gegensatz dazu bewirkt die Zugabe von Pluronic® F-68 (0,2 - 1,0 %) sowohl eine Steigerung der Viabiliät als auch der Proliferationsrate von Caco-2 Zellen. Besonders deutlich zeigte sich der proliferationsfördernde Effekt bei Zellen, die nach dem Auftauen in 0,2 1,0 % Pluronic® F-68-hältigem Zellkulturmedium kultiviert wurden. Die Zell-proliferation stieg dabei um das bis zu 2,5-fache im Vergleich zu jenen Zellen, die in Tensid-freiem Medium kultiviert wurden. Die Steigerung der Nanopartikelassoziation an die Zellen in Gegenwart der Tenside Tween® 20, Tween® 80 und Pluronic® F-68 stellt einen interessanten Ausgangspunkt dar, um möglicherweise die Resorptionsrate von Nanopartikeln zu erhöhen

Server Hosting Services for Online Gaming

U ovom radu obrađena je tema Server Hosting usluga za online igre. Spomenuto je osnovno o internetu i njegovim dijelovima. Obrađeni su WWW, Internet protokoli i Internet servisi odnosno usluge. Također obrađene su i online igre, njihova povijest te vrste i žanrovi. Nakon toga dolazi ono najbitnije, a to su Hosting usluge. Obrađene su njihove vrste, a one su Shared, VPS, Dedicated i Cloud hosting usluge. Nakon toga slijede liste pružatelja usluga pojedinih vrsta hostinga u odnosu na mogućnost hostinga online igara, njihova cijena i performanse

Silicon Carbide Timepix3 detector for quantum-imaging detection and spectral tracking of charged particles in wide range of energy and field-of-view

The hybrid architecture of the Timepix (TPX) family of detectors enables the use of different semiconductor sensors, most commonly silicon (Si), as well as high-density materials such as Cadmium Telluride (CdTe) or Gallium Arsenide (GaAs). For this purpose, we explore the potential of a silicon carbide (SiC) sensor bump-bonded on a Timepix3 detector as a radiation imaging and particle tracking detector. SiC stands as a radiation-hard material also with the ability to operate at elevated temperatures up to several hundreds of degrees Celsius. As a result, this sensor material is more suitable for radiation harsh environments compared to conventional e.g., Si sensors. In this work, we evaluate the response for precise radiation spectrometry and high-resolution particle tracking of newly developed SiC Timepix3 detector which is built and operated as a compact radiation camera MiniPIX-Timepix3 with integrated readout electronics. Calibration measurements were conducted with mono-energetic proton beams with energies of 13, 22, and 31 MeV at the U-120M cyclotron at the Nuclear Physics Institute Czech Academy of Science (NPI CAS), Prague, as well as 100 and 226 MeV at the Proton Therapy Center Czech (PTC) in Prague. High-resolution pattern recognition analysis and single-particle spectral tracking are used for detailed inspection and understanding of the sensor response. Results include distributions of deposited energy and linear energy transfer (LET) spectra. The spatial uniformity of the pixelated detector response is examined in terms of homogeneously distributed deposited energy.Comment: 9 pages, proceedings iWoRi

ICT Assistive Technology for Children with Disabilities

U radu je obrađena tema teškoća u razvoju i ICT asistivnih tehnologija za pomoć djeci s različitim teškoćama. Kod djece s oštećenjem vida, sluha, govorno-glasovnim teškoćama, motoričkim teškoćama, zdravstvenim teškoćama, poremećajima u ponašanju i neurološkim oštećenjima primjenjuju se asistivni alati i pomagala. Kako je tehnologija napredovala, tako su i medicina i asistivne tehnologije držale korak s njom. Teškoće se ranije i češće otkrivaju zbog napretka tehnologije, a na isti način sve su se više razvijale i asistivne tehnologije. Međutim, glavni nedostatak većine asistivnih tehnologija jest njihova cijena, no prednost je mnogo veća, a to je mogućnost inkluzije djece s teškoćama u razvoju kako bi im se omogućili redovni uvjeti života. Cilj rada je prikazati pojedine asistivne alate odnosno tehnologiju za djecu s različitim teškoćama u razvoju koji su podrška u nastavnom procesu, kao i pregled literature s kritičkim osvrtom u tom smislu.This paper deals with the topic of developmental disabilities and ICT assistive technologies to help children with various disabilities. Assistive tools and aids are used for children with visual impairment, hearing impairment, speech and voice difficulties, motor difficulties, health difficulties, behavioral disorders and neurological impairments. As technology has advanced, medicine and assistive technologies have kept pace with it. Difficulties are detected earlier and more often due to the progress of technology, and in the same way assistive technologies have been increasingly developed. However, the main disadvantage of most assistive technologies is their price, but the advantage is much greater, which is the possibility of including children with developmental disabilities in order to provide them with regular living conditions. The aim of this paper is to present certain assistive tools or technology for children with various developmental disabilities that are a support in the teaching process, as well as a literature review with a critical review in this sense

ICT Assistive Technology for Children with Disabilities

U radu je obrađena tema teškoća u razvoju i ICT asistivnih tehnologija za pomoć djeci s različitim teškoćama. Kod djece s oštećenjem vida, sluha, govorno-glasovnim teškoćama, motoričkim teškoćama, zdravstvenim teškoćama, poremećajima u ponašanju i neurološkim oštećenjima primjenjuju se asistivni alati i pomagala. Kako je tehnologija napredovala, tako su i medicina i asistivne tehnologije držale korak s njom. Teškoće se ranije i češće otkrivaju zbog napretka tehnologije, a na isti način sve su se više razvijale i asistivne tehnologije. Međutim, glavni nedostatak većine asistivnih tehnologija jest njihova cijena, no prednost je mnogo veća, a to je mogućnost inkluzije djece s teškoćama u razvoju kako bi im se omogućili redovni uvjeti života. Cilj rada je prikazati pojedine asistivne alate odnosno tehnologiju za djecu s različitim teškoćama u razvoju koji su podrška u nastavnom procesu, kao i pregled literature s kritičkim osvrtom u tom smislu.This paper deals with the topic of developmental disabilities and ICT assistive technologies to help children with various disabilities. Assistive tools and aids are used for children with visual impairment, hearing impairment, speech and voice difficulties, motor difficulties, health difficulties, behavioral disorders and neurological impairments. As technology has advanced, medicine and assistive technologies have kept pace with it. Difficulties are detected earlier and more often due to the progress of technology, and in the same way assistive technologies have been increasingly developed. However, the main disadvantage of most assistive technologies is their price, but the advantage is much greater, which is the possibility of including children with developmental disabilities in order to provide them with regular living conditions. The aim of this paper is to present certain assistive tools or technology for children with various developmental disabilities that are a support in the teaching process, as well as a literature review with a critical review in this sense

Metodika zkouseni "hydrostaticke ulozeni brousiciho hridele brusky BUC 63 C CNC.

Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi