Evidence of cross-cutting and redox reaction in Khatyrka meteorite reveals metallic-Al minerals formed in outer space

We report on a fragment of the quasicrystal-bearing CV3 carbonaceous chondrite Khatyrka recovered from fine-grained, clay-rich sediments in the Koryak Mountains, Chukotka (Russia). We show higher melting-point silicate glass cross-cutting lower melting-point Al-Cu-Fe alloys, as well as unambiguous evidence of a reduction-oxidation reaction history between Al-Cu-Fe alloys and silicate melt. The redox reactions involve reduction of FeO and SiO_2 to Fe and Fe-Si metal, and oxidation of metallic Al to Al_2O_3, occurring where silicate melt was in contact with Al-Cu-Fe alloys. In the reaction zone, there are metallic Fe and Fe-Si beads, aluminous spinel rinds on the Al-Cu-Fe alloys, and Al_2O_3 enrichment in the silicate melt surrounding the alloys. From this and other evidence, we demonstrate that Khatyrka must have experienced at least two distinct events: first, an event as early as 4.564 Ga in which the first Al-Cu-Fe alloys formed; and, second, a more recent impact-induced shock in space that led to transformations of and reactions between the alloys and the meteorite matrix. The new evidence firmly establishes that the Al-Cu-Fe alloys (including quasicrystals) formed in outer space in a complex, multi-stage process

A Short Review of Iron Metabolism and Pathophysiology of Iron Disorders

Iron is a vital trace element for humans, as it plays a crucial role in oxygen transport, oxidative metabolism, cellular proliferation, and many catalytic reactions. To be beneficial, the amount of iron in the human body needs to be maintained within the ideal range. Iron metabolism is one of the most complex processes involving many organs and tissues, the interaction of which is critical for iron homeostasis. No active mechanism for iron excretion exists. Therefore, the amount of iron absorbed by the intestine is tightly controlled to balance the daily losses. The bone marrow is the prime iron consumer in the body, being the site for erythropoiesis, while the reticuloendothelial system is responsible for iron recycling through erythrocyte phagocytosis. The liver has important synthetic, storing, and regulatory functions in iron homeostasis. Among the numerous proteins involved in iron metabolism, hepcidin is a liver-derived peptide hormone, which is the master regulator of iron metabolism. This hormone acts in many target tissues and regulates systemic iron levels through a negative feedback mechanism. Hepcidin synthesis is controlled by several factors such as iron levels, anaemia, infection, inflammation, and erythropoietic activity. In addition to systemic control, iron balance mechanisms also exist at the cellular level and include the interaction between iron-regulatory proteins and iron-responsive elements. Genetic and acquired diseases of the tissues involved in iron metabolism cause a dysregulation of the iron cycle. Consequently, iron deficiency or excess can result, both of which have detrimental effects on the organism

The host immune response to gastrointestinal nematode infection in sheep

non peer reviewedGastrointestinal nematode infection represents a major threat to the health, welfare and productivity of sheep populations worldwide. Infected lambs have a reduced ability to absorb nutrients from the gastrointestinal tract, resulting in morbidity and occasional mortality. The current chemo-dominant approach to nematode control is considered unsustainable due to the increasing incidence of anthelmintic resistance. In addition there is growing consumer demand for food products from animals not subjected to chemical treatment. Future mechanisms of nematode control must rely on alternative, sustainable strategies such as vaccination or selective breeding of resistant animals. Such strategies take advantage of the host's natural immune response to nematodes. The ability to resist gastrointestinal nematode infection is considered to be dependent on the development of a protective acquired immune response; although the precise immune mechanisms involved in initiating this process remain to be fully elucidated. In this paper current knowledge on the innate and acquired host immune response to gastrointestinal nematode infection in sheep and the development of immunity is reviewed.We gratefully acknowledge funding support for the research in our laboratories from the Teagasc Walsh Fellowship Programme, the Allan and Grace Kay Overseas Scholarship and the EC-funded FP7 Programme. We also thank the BBSRC Animal Health Research Club for funding part of this research (grant BB/l004070/1

Coeval extensional shearing and lateral underflow during Late Cretaceous core complex development in the Niğde Massif, Central Anatolia, Turkey

27 p.International audienceThe Nig¢de Massif, at the southern tip of the Central Anatolian Crystalline Complex, consists of two structural units. Foliations in the lower unit define a dome cored by migmatites. The contact between the two units bears all the elements of a ductile to brittle extensional detachment. Hence the Nig¢de Massif represents an extensional metamorphic core complex. Top-to-NE/ENE shearing at higher levels of the lower unit relates to displacement along the detachment. Deeper levels of the lower unit display hightemperature top-to-SSW ductile shearing. The two shearing deformations show a difference in the mean trend of stretching lineations of up to 58. New 40Ar/39Ar ages combined with previously published data enable us to infer that the two shears were contemporaneous. In our favored interpretation, oblique shearing in the core of the dome reflects lateral underflow, i.e., horizontal flowing of the lower crust in a direction highly oblique to the direction of extension. As a result of the interaction between lateral underflow and downdip shearing along the overlying detachment, distinct structural domains are expected to exist within the migmatitic part of the core complex, with observed counterparts in the Nig¢de dome. Lateral underflow may reflect ''inward'' flow on the scale of the core complex. Regional-scale channel flow is an alternative that would better account for the record of non-coaxial deformation in the core of the dome. More generally, we suspect that the development of lateral underflow in a metamorphic core complex more likely reflects regional channel flow, rather than local inward flow

Tectonic setting of the Sandia pluton: An orogenic 1.4 Ga granite in New Mexico

Structural studies of the circa 1.42 Ga Sandia pluton and its aureole document significant deformation synchronous with pluton emplacement and call into question the “anorogenic” label associated with this and other 1.4 Ga granites in the southwestern United States. The SE margin of the pluton is a 1‐ to 2‐km‐wide NW dipping ductile shear zone. Field and microstructural observations (melt‐filled shear bands, high‐temperature dynamic recrystallization of K‐feldspar megacrysts, and crosscutting pegmatite dikes) indicate that top‐to‐the‐NW (normal) movement in the shear zone took place in the presence of melt. Subparallel magmatic fabrics north of and structurally above the shear zone contain kinematic indicators consistent with top‐to‐the‐NW shear sense, suggesting that over large regions of the pluton, magmatic flow mimicked solid‐state strain. In the northern aureole, contact metamorphic aluminosilicate porphyroblasts grew during the formation of a NE striking crenulation cleavage (S3) and related folds of late‐stage pegmatite dikes. These features document the synchroneity of magma emplacement, shortening, and metamorphism and indicate that the Sandia pluton is syntectonic, not anorogenic. We interpret the kinematic consistency of structural elements from the base of the pluton, the interior of the pluton, and the northern aureole to reflect a regional (larger than the pluton) strain field and suggest that the “orogeny” recorded in and around the Sandia pluton involved a three dimensional strain field with subhorizontal extension (N–S) and contraction (E–W) directions. N–S extension is documented by the orientation of mineral lineations and movement directions in the basal shear zone and in high‐strain zones in the northern aureole and by the orientations of tabular pegmatite and aplite dikes in the pluton and aureole. East to SE shortening is documented in the northern aureole by orientations of folded pegmatite dikes and associated S3 crenulation cleavage, and east to SE shortening (or least extension) directions in the pluton proper are documented by the intersections of orthogonal dikes. Thus emplacement of the Sandia pluton is interpreted to record a snapshot of regional strains inboard of an active plate margin, rather than local strains generated by emplacement

Towards 'in vitro' produced germline stem cells in the bovine

Bovine spermatogonial stem cells (SSCs) have potential to be used in advanced reproductive technologies such as testis cell transplantation, where identification and purification of large numbers of SSCs is required. There are at least two possible sources of SSCs: isolation from the testis, or in vitro differentiation from pluripotent stem cells. The long-term goal of this thesis was to work towards the generation of SSCs from bovine somatic cells using induced pluripotent stem (iPS) cell technology. In order to do so, it was first important to characterise molecular markers expressed by bovine SSCs to allow for their identification in culture, and secondly to explore the feasibility of producing bovine iPS cells. In order to achieve the first goal, a screening platform was developed based on comparative analysis of gene expression levels in SSC enriched and depleted cell populations. Expression of established testis cell markers was used to confirm the validity of the screening platform. This method was then used to examine expression of candidate spermatogonial markers in the bovine testis. STRA8, KIT, GFRA1, CLDN8, DDX6 and NAP1L4 were shown to be putative markers for bovine spermatogonia. Further analysis of CLDN8 showed expression by both a subset of spermatogonia and a subset of Sertoli cells, leading to the hypothesis that CLDN8 plays a role in the maintenance of SSCs in the SSC niche. Reprogramming of bovine somatic cells was undertaken by introducing canonical reprogramming factors through a lentiviral vector. Initial experiments found that the reprogramming protocol was sufficient to produce cells exhibiting stem cell-like characteristics. Analysis of these cells indicated partial reprogramming had been achieved. A number of small molecules were then tested for their ability to enhance the success of cell reprogramming. A combination of three small molecules was found to accelerate the kinetics of the reprogramming process and also promoted further reprogramming where cells could differentiate to the three different germ layers. Further research is now required to define the optimal culture conditions for the maintenance and expansion of bovine pluripotent cells in long term culture, and to test whether they can be differentiated towards the germ line