KONSTRUKSI TANDA-TANDA NASIONALISME DALAM VIDEO KLIP MUSIK (Analisis Semiotika Charles Sander Peirce Dalam Video Klip Wonderland Indonesia By : Alffy Rev Ft. Novia Bachmid Chapter 1)

This research aims to gain an understanding of the message content in Wonderland Indonesia Chapter 1. Wonderland Indonesia is a creation by Indonesian youth, and its purpose is to serve as a gift for the 76th anniversary of the nation's independence. It trended as number 1 on YouTube in the music category, and received many positive comments stating that watching the Wonderland Indonesia video can evoke a sense of nationalism. The purpose of creating the Wonderland Indonesia music video is to reintroduce Indonesian culture and encourage young people to once again memorize regional songs, presented with an EDM (Electronic Dance Music) music arrangement. This research employs a qualitative, descriptive approach, with the analytical tool being Charles Sender Peirce's theory. The findings of this study reveal that Indonesia, as an archipelagic nation, possesses stunning natural beauty and a heritage that has endured through the ages, making it a culturally rich and multicultural country. The research also highlights Indonesia's history of striving for independence and its classical era, such as the Majapahit era. It conveys a message to the younger generation to always serve their nation

Konglomeratrabatten - myte eller realitet? : forklaring av verdieffekter ved urelatert diversifisering gjennom bruk og sammenfatning av tidligere studier

Formålet med denne oppgaven er å forklare verdieffektene av urelatert diversifisering gjennom bruk og sammenfatning av tidligere studier. I første del beskriver og systematiserer vi selskapsfaktorer, som spesielt innvirker på verdien av et konglomerat, i ulike perspektiver. Andre del fokuserer på vanlige målemetoder for å estimere en eventuell konglomeratrabatt, og hva empirisk forskning sier om rabatten. Tverrsnittanalyser viser en signifikant konglomeratrabatt i størrelsesorden 3-19 %. Videre kritiseres svakheter ved målemetodene, som at benyttede data ofte er for unøyaktige og at fundamentale selskapsforhold ikke tas hensyn til. Rabatten er reell i mange tilfeller, men skyldes ofte andre forhold enn diversifiseringen i seg selv. I siste del av utredningen sammenfatter og analyserer vi tidligere gjennomgått teori. Den største, og mest sannsynlige, verdidriveren og verdiforringeren i et konglomerat identifiseres til henholdsvis ressursbasert diversifisering og agentproblemer. Vi finner også at selskapers optimale diversifiseringsnivå varierer stort, og bestemmes av karakteristikker ved selskapet og dets omgivelser

Comparative profiling of the transcriptional response to iron restriction in six serotypes of Actinobacillus pleuropneumoniae with different virulence potential

Background Comparative analysis of gene expression among serotypes within a species can provide valuable information on important differences between related genomes. For the pig lung pathogen Actinobacillus pleuropneumoniae, 15 serotypes with a considerable variation in virulence potential and immunogenicity have been identified. This serotypic diversity can only partly be explained by amount of capsule and differences in the RTX toxin genes in their genomes. Iron acquisition in vivo is an important bacterial function and in pathogenic bacteria, iron-limitation is often a signal for the induction of virulence genes. We used a pan-genomic microarray to study the transcriptional response to iron restriction in vitro in six serotypes of A. pleuropneumoniae (1, 2, 3, 5b, 6, and 7), representing at least two levels of virulence. Results In total, 45 genes were significantly (p <0.0001) up-regulated and 67 genes significantly down-regulated in response to iron limitation. Not previously observed in A. pleuropneumoniae was the up-regulation of a putative cirA-like siderophore in all six serotypes. Three genes, recently described in A. pleuropneumoniae as possibly coding for haemoglobin-haptoglobin binding proteins, displayed significant serotype related up-regulation to iron limitation. For all three genes, the expression appeared at its lowest in serotype 3, which is generally considered one of the least virulent serotypes of A. pleuropneumoniae. The three genes share homology with the hmbR haemoglobin receptor of Neisseria meningitidis, a possible virulence factor which contributes to bacterial survival in rats. Conclusions By comparative analysis of gene expression among 6 different serotypes of A. pleuropneumoniae we identified a common set of presumably essential core genes, involved in iron regulation. The results support and expand previous observations concerning the identification of new potential iron acquisition systems in A. pleuropneumoniae, showing that this bacterium has evolved several strategies for scavenging the limited iron resources of the host. The combined effect of iron-depletion and serotype proved to be modest, indicating that serotypes of both moderate and high virulence at least in vitro are reacting almost identical to iron restriction. One notable exception, however, is the haemoglobin-haptoglobin binding protein cluster which merits further investigation

Minat Berwirausaha Ditinjau Dari Motivasi Berwirausaha Dan Prestasi Belajar Mata Pelajaran Kewirausahaan Pada Siswa Kelas Xi Program Keahlian Karawitan Dan Seni Tari Smk Negeri 8 Surakarta Tahun Ajaran 2014/2015

Tujuan penelitian ini adalah untuk mengetahui: 1). Pengaruh motivasi berwirausaha terhadap minat berwirausaha pada siswa kelas XI program keahlian karawitan dan seni tari SMK Negeri 8 Surakarta tahun ajaran 2014/2015. 2). Pengaruh prestasi hasil belajar mata pelajaran kewirausahaan terhadap minat berwirausaha pada siswa kelas XI program keahlian karawitan dan seni tari SMK Negeri 8 Surakarta tahun ajaran 2014/2015. 3). Pengaruh motivasi berwirausaha dan prestasi hasil belajar mata pelajaran kewirausahaan terhadap minat berwirausaha pada siswa kelas XI program keahlian karawitan dan seni tari SMK Negeri 8 Surakarta tahun ajaran 2014/2015. Penelitian ini termasuk penelitian kuantitatif asosiatif yang kesimpulannya diperoleh berdasarkan pada hasil analisis statistik.Penelitian ini mengambil lokasi SMK NEGERI 8 SURAKARTA. Teknik pengumpulan data dengan menggunakan dokumen dan angket yang telah diuji coba dengan uji normalitas, uji reliabilitas dan uji multikolinieritas. Teknik analisis yang digunakan adalah regresi berganda. Hasil dari analisis regresi diperoleh persamaan regresi; Y = 20,500+ 0,196X1 + 0,108X2, yang artinya minat berwirausaha dipengaruhi oleh motivasi berwirausaha dan prestasi belajar kewirausahaan, berdasarkan analisis dan pembahasan dapat disimpulkan bahwa: 1). Motivasi berwirausaha berpengaruh positif terhadap minat berwirausaha, hal ini berdasarkan hasil analisis regresi linier berganda (uji t) diketahui thitung > ttabel, yaitu 3.447 > 1,984 dan nilai signifikansi < 0,05, yaitu 0,001. 2). Prestasi belajar kewirausahaan berpengaruh positif terhadap minat berwirausaha, hal ini berdasarkan hasil analisis regresi linier berganda (uji t) diketahui t hitung > ttabel , yaitu 3,007 > 1,984 dan nilai signifikansi < 0,05, yaitu 0,003. 3). Motivasi berwirausaha dan prestasi belajar kewirausahaan secara bersama berpengaruh positif terhadap minat berwirausaha, hal ini berdasarkan hasil analisis regresi linier berganda (uji F) diketahui F hitung > Ftabel yaitu 14,858 > 3,07 dengan nilai signifikansi < 0,05 yaitu 0,000. Variabel motivasi berwirausaha memberikan sumbangan efektif 12,0 %. Variabel prestasi belajar kewirausahaan memberikan sumbangan efektif 9,7%. Sehinggan total sumbangan keduanya sebesar 21,7% sedangkan 78,3% lainnya dipengaruhi oleh variabel lain yang tidak diteliti oleh peneliti

An evaluation of the ability of Dichelobacter nodosus to survive in soil

<p>Abstract</p> <p>Background</p> <p><it>Dichelobacter nodosus</it> is the causative agent of footrot in sheep. The survival of the bacterium in soil is of importance for the epidemiology of the disease. The investigation evaluates the survival of <it>D. nodosus</it> in soil with and without added hoof powder stored under different temperatures.</p> <p>Results</p> <p>An experimental setup was used with bacteriological culture and real-time polymerase chain reaction (PCR), and the results indicate that the bacteria can survive in soil for longer time than previously expected. The survival time was found to be dependent on temperature and the addition of hoof powder to the soil, with the longest survival time estimated to be 24 days in soil samples with hoof powder stored at 5°C.</p> <p>Conclusion</p> <p>Our findings indicate that the survival time of <it>D. nodosus</it> and its ability to infect susceptible sheep on pasture under different climatic conditions should be studied further.</p

Sampling and Pooling Methods for Capturing Herd Level Antibiotic Resistance in Swine Feces using qPCR and CFU Approaches

The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic resistance genes in total DNA from swine fecal samples that were obtained using different sampling and pooling methods. In parallel, the number of antibiotic resistant coliform indicator bacteria was determined in the same swine fecal samples. The results showed that the qPCR assays were capable of detecting differences in antibiotic resistance levels in individual animals that the coliform bacteria colony forming units (CFU) could not. Also, the qPCR assays more accurately quantified antibiotic resistance genes when comparing individual sampling and pooling methods. qPCR on pooled samples was found to be a good representative for the general resistance level in a pig herd compared to the coliform CFU counts. It had significantly reduced relative standard deviations compared to coliform CFU counts in the same samples, and therefore differences in antibiotic resistance levels between samples were more readily detected. To our knowledge, this is the first study to describe sampling and pooling methods for qPCR quantification of antibiotic resistance genes in total DNA extracted from swine feces