Near field communication mit Arduino

Das vorliegende Arbeitspapier beschäftigt sich mit dem Themenkomplex Near Field Communication (NFC). Diese Technologie ist viel diskutiert. Ihr wird seit Jahren vorausgesagt, dass sie die Anwendungsmöglichkeiten von Smartphones erweitern wird.1 Zahlreicher Interessengruppen suchen Wege und Möglichkeiten der kommerziellen Nutzung dieser Technologie.2 Aus Interesse an diesem vielfältigen Themenspektrum und dem noch offenen Entwicklungsbedarf in einigen Aspekten wurde dieses Arbeitspapier erstellt. In diesem sollen die Möglichkeiten der NFC-Technologie erörtert und geklärt werden. Auf Grund der Interaktionen von NFC mit anderen Geräten und dem Austausch von Informationen, lag der Schwerpunkt dieser Arbeit in der Ermittlung und dem Test von Schnittstellen. Eine weitere Zielsetzung ist es, verschiedene Schlüsselfunktionen der Technologie, wie Kommunikation zwischen NFC tauglichen Geräten, auf ihre Umsetzbarkeit zu testen. Vor dem Hintergrund wird im ersten Teil dieses Papiers die Theorie zu Near Field Communiction in der Theorie betrachtet. Hierbei werden die allgemeine Funktionsweise, die technische Funktionsweise und verschiedene Protokolle dargestellt. Des Weiteren wird auf den aktuellen Stand der Technik eingegangen. Um die Praxistauglichkeit von NFC einschätzen zu können, werden im Anschluss die Verbreitung und die Sicherheit der Technologie analysiert. Der anschließende, praktische Teil stellt den Projektverlauf dar. Um in die NFC Thematik einzusteigen, wurden ein NFC fähiges Mobiltelefon, eine Arduino-Mikrocontrollerboard mit NFC-Shield und ein NFC-Tag verwendet. Im Projektverlauf wird beschrieben, wie diese miteinander agieren können und welche Funktionen aus dem theoretischen Teil bei diesen Geräten bereits implementiert wurden. Hierbei wird auf die, beim Testen der Funktion, gestoßen Schwierigkeiten eingegangen. Auf Grundlage des zuvor dargestellten Wissens werden Lösungen der zu bewältigenden Schwierigkeiten skizziert. Zum Abschluss geht diese Arbeit auf die Ergebnisse der Programmierung mit dem Arduino ein

Spatially distributed multipartite entanglement enables Einstein-Podolsky-Rosen steering of atomic clouds

A key resource for distributed quantum-enhanced protocols is entanglement between spatially separated modes. Yet, the robust generation and detection of nonlocal entanglement between spatially separated regions of an ultracold atomic system remains a challenge. Here, we use spin mixing in a tightly confined Bose-Einstein condensate to generate an entangled state of indistinguishable particles in a single spatial mode. We show experimentally that this local entanglement can be spatially distributed by self-similar expansion of the atomic cloud. Spatially resolved spin read-out is used to reveal a particularly strong form of quantum correlations known as Einstein-Podolsky-Rosen steering between distinct parts of the expanded cloud. Based on the strength of Einstein-Podolsky-Rosen steering we construct a witness, which testifies up to genuine five-partite entanglement.Comment: 27 pages, 4 figures, 6 supplementary figure

Complete Genome Sequence of the Type Strain Corynebacterium testudinoris DSM 44614, Recovered from Necrotic Lesions in the Mouth of a Tortoise

The complete genome sequence of the type strain Corynebacterium testudinoris DSM 44614 from the mouth of a tortoise comprises 2,721,226 bp with a mean G+C content of 63.14%. The automatic annotation of the genome sequence revealed 4 rRNA operons, 51 tRNA genes, 7 other RNA genes, and 2,561 protein-coding regions.Medical Microbiology and Genomics fund (eKVV 200937)Germany. Federal Ministry of Education and Research (German Network for Bioinformatics Intrastructure Initiative FKZ 031A533A

Virulence Factor Genes Detected in the Complete Genome Sequence of Corynebacterium uterequi DSM 45634, Isolated from the Uterus of a Maiden Mare

The complete genome sequence of the type strain Corynebacterium uterequi DSM 45634 from an equine urogenital tract specimen comprises 2,419,437 bp and 2,163 protein-coding genes. Candidate virulence factors are homologs of DIP0733, DIP1281, and DIP1621 from Corynebacterium diphtheriae and of sialidase precursors from Trueperella pyogenes and Chlamydia trachomatis.Medical Microbiology and Genomics fund (eKVV 200937)Germany. Federal Ministry of Education and Research (German Network for Bioinformatics Intrastructure Initiative FKZ 031A533A

Graded inhibition of oncogenic Ras-signaling by multivalent Ras-binding domains

BACKGROUND: Ras is a membrane-associated small G-protein that funnels growth and differentiation signals into downstream signal transduction pathways by cycling between an inactive, GDP-bound and an active, GTP-bound state. Aberrant Ras activity as a result of oncogenic mutations causes de novo cell transformation and promotes tumor growth and progression. RESULTS: Here, we describe a novel strategy to block deregulated Ras activity by means of oligomerized cognate protein modules derived from the Ras-binding domain of c-Raf (RBD), which we named MSOR for multivalent scavengers of oncogenic Ras. The introduction of well-characterized mutations into RBD was used to adjust the affinity and hence the blocking potency of MSOR towards activated Ras. MSOR inhibited several oncogenic Ras-stimulated processes including downstream activation of Erk1/2, induction of matrix-degrading enzymes, cell motility and invasiveness in a graded fashion depending on the oligomerization grade and the nature of the individual RBD-modules. The amenability to accurate experimental regulation was further improved by engineering an inducible MSOR-expression system to render the reversal of oncogenic Ras effects controllable. CONCLUSION: MSOR represent a new tool for the experimental and possibly therapeutic selective blockade of oncogenic Ras signals

Effects of ceftiofur treatment on the susceptibility of commensal porcine E.coli – comparison between treated and untreated animals housed in the same stable

Background Healthy farm animals have been found to act as a reservoir of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli). Therefore, the objective of the study was to determine the input of antimicrobial active ceftiofur metabolites in the stable via faeces and urine after intramuscular administration of the drug to pigs and the elucidation of the Escherichia coli ESBL resistance pattern of treated and untreated pigs housed in the same barn during therapy. Methods For determination of the minimal inhibitory concentration (MIC) the method of microdilutionaccording to the recommended procedure of the Clinical and Laboratory Standards Institute was used. Inaddition to that, a qualitative determination was performed by agar dilution. Unsusceptible E. coli speciesselected via agar dilution with cefotaxime were confirmed by MALDI-TOF and ESBL encoding genes wereidentified by PCR. The amounts of ceftiofur measured as desfuroylceftiofur (DFC) in the different probes (plasma, urine, faeces and dust) were analysed by UPLC-MS/MS. Results In a first experiment two groups of pigs (6 animals per group) were housed in the same barn in two separated boxes. One group (group B) were treated with ceftiofur according to the licence (3 mg/kg administered intramuscularly (i.m.) on three consecutive days, day 1–3). During a second treatment period (day 29–31) an increased rate of ESBL resistant E. coli was detectable in these treated pigs and in the air of the stable. Moreover, the second group of animals (group A) formerly untreated but housed for the whole period in the same stable as the treated animals revealed increased resistance rates during their first treatment (day 45–47) with ceftiofur. In order to investigate the environmental input of ceftiofur during therapy and to simulate oral uptake of ceftiofur residues from the air of the stable a second set of experiments were performed. Pigs (6 animals) were treated with an interval of 2 weeks for 3 days with different doses of ceftiofur (3 mg/kg, 1 mg/kg and 0.3 mg/kg i.m.) as well as with 3 mg/kg per os) and the renal and biliary excretion of ceftiofur as its active metabolite were measured in comparison to the plasma levels. In addition to that, probes of the sedimentation dust and the air of the stable were analysed for drug residues. Conclusion The present study shows that treatment of several animals in a stable with ceftiofur influences the resistance pattern of intestinal Escherichia coli of the treated as well as untreated animals housed in the same stable. During therapy with the drug which was administered by injection according to the licence we detected nameable amounts of ceftiofur and its active metabolites in the dust and air of the stable

Comparative transcriptomics of stickleback immune gene responses upon infection by two helminth parasites, Diplostomum pseudospathaceum and Schistocephalus solidus

Immune systems of vertebrates are much more diverse than previously thought, in particular at the base of the vertebrate clade. RNA-seq was used to describe in detail the transcriptomic response of stickleback hosts to infection by two helminth parasites, the trematode . Diplostomum pseudospathaceum (2 genotypes plus a genotype mix) and the cestode . Schistocephalus solidus. Based on a global transcription profiling, we present immune genes that are active during chronic or multiple repeated infection. We found that the transcription profiles of . D. pseudospathaceum genotypes were as divergent as those of the two parasite species. When comparing the host immune response, only 5 immune genes were consistently upregulated upon infection by both species. These genes indicated a role for enhanced toll like receptor (TLR) activity (CTSK, CYP27B1) and an associated positive regulation of macrophages (CYP27B1, THBS1) for general helminth defense. We interpret the largely differentiated gene expression response among parasite species as general redundancy of the vertebrate immune system, which was also visible in genotype-specific responses among the different . D. . pseudospathaceum infections. The present study provides the first evidence that IL4-mediated activation of T-helper lymphocyte cells is also important in anti-helminthic immune responses of teleost fish

Kinematic structure of massive star-forming regions. I. accretion along filaments

Context. The mid- and far-infrared view on high-mass star formation, in particular with the results from the Herschel space observatory, has shed light on many aspects of massive star formation. However, these continuum studies lack kinematic information. Aims: We study the kinematics of the molecular gas in high-mass star-forming regions. Methods: We complemented the PACS and SPIRE far-infrared data of 16 high-mass star-forming regions from the Herschel key project EPoS with N2H+ molecular line data from the MOPRA and Nobeyama 45 m telescope. Using the full N2H+ hyperfine structure, we produced column density, velocity, and linewidth maps. These were correlated with PACS 70 μm images and PACS point sources. In addition, we searched for velocity gradients. Results: For several regions, the data suggest that the linewidth on the scale of clumps is dominated by outflows or unresolved velocity gradients. IRDC 18454 and G11.11 show two velocity components along several lines of sight. We find that all regions with a diameter larger than 1 pc show either velocity gradients or fragment into independent structures with distinct velocities. The velocity profiles of three regions with a smooth gradient are consistent with gas flows along the filament, suggesting accretion flows onto the densest regions. Conclusions: We show that the kinematics of several regions have a significant and complex velocity structure. For three filaments, we suggest that gas flows toward the more massive clumps are present

Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity

Moghadam MS, Albersmeier A, Winkler A, et al. Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity. BMC Genomics. 2016;17(1): 117