Whole genome sequencing of rabies virus from archived human and canine brain tissues from southern India using Nanopore technology

Rabies, a fatal zoonotic viral encephalitis kills tens of thousands of people each year in India, representing one third of the estimated global rabies burden. A detailed understanding of the molecular epidemiology of the Rabies lyssavirus (RABV) in India is limited due to the low availability of sequences from the country. In this study we examined the feasibility of direct sequencing of clinical samples (archived human and canine brain tissues, 10 each), for detection and recovery of whole genome sequences of RABV, on the MinION – single molecule nanopore sequencing device. Using an amplicon-based approach, complete RABV genomes were recovered from all the 20 samples, archived from 2003 to 2019, from 4 southern states of India. Phylogenetic analysis revealed that all 20 sequences from this study belonged to the Arctic-like (AL) 1a lineage. Divergence estimation of sequences revealed that all the circulating RABV strains within India are diverged in last 60 years from the previous ancestor. The genetic relatedness and close clustering of the sequences in the study suggests the continued propagation of AL1a in the region, across state boundaries. Genomic surveillance can provide unique insights into rabies spread and persistence that can direct control efforts in India.</jats:p

Filovirus-reactive antibodies in humans and bats in Northeast India imply zoonotic spillover

10.1371/journal.pntd.0007733PLoS Neglected Tropical Diseases1310e000773

Magnetic enhancement of g-Fe2O3 nanoparticles by sonochemical coating

We show that sonochemistry is an efficient and facile route for quantitative coating of γ-Fe2O3 nanoparticles with octadecyltrihydrosilane (OTHS, CH3(CH2)17SiH3). The presence of C−H stretching (2950−2850 cm-1) and bending (1475−1375 cm-1) bands, and the absence of peaks at 2150 and 925 cm-1 (Si−H stretching and bending respectively) confirm the presence of grafted hydrocarbon chains in the irradiated sample, hence the reaction of OTHS with γ-Fe2O3 nanoparticles. The coated nanoparticles show increased magnetization compared to the uncoated ones. X-ray diffraction, magnetization studies, Mössbauer spectra, and electron paramagnetic resonance spectra reveal that the magnetic ordering is more prominent in the OTHS-coated γ-Fe2O3 sample because of improved crystallinity