Environmental control of microtubule-based bidirectional cargo-transport

Inside cells, various cargos are transported by teams of molecular motors. Intriguingly, the motors involved generally have opposite pulling directions, and the resulting cargo dynamics is a biased stochastic motion. It is an open question how the cell can control this bias. Here we develop a model which takes explicitly into account the elastic coupling of the cargo with each motor. We show that bias can be simply controlled or even reversed in a counterintuitive manner via a change in the external force exerted on the cargo or a variation of the ATP binding rate to motors. Furthermore, the superdiffusive behavior found at short time scales indicates the emergence of motor cooperation induced by cargo-mediated coupling

Fluctuation effects in bidirectional cargo transport

We discuss a theoretical model for bidirectional cargo transport in biological cells, which is driven by teams of molecular motors and subject to thermal fluctuations. The model describes explicitly the directed motion of the molecular motors on the filament. The motor-cargo coupling is implemented via linear springs. By means of extensive Monte Carlo simulations we show that the model describes the experimentally observed regimes of anomalous diffusion, i.e. subdiffusive behavior at short times followed by superdiffusion at intermediate times. The model results indicate that subdiffuse regime is induced by thermal fluctuations while the superdiffusive motion is generated by correlations of the motors' activity. We also tested the efficiency of bidirectional cargo transport in crowded areas by measuring its ability to pass barriers with increased viscosity. Our results show a remarkable gain of efficiency for high viscosities.Comment: 10 pages, 6 figure

Motility states in bidirectional cargo transport

Intracellular cargos which are transported by molecular motors move stochastically along cytoskeleton filaments. In particular for bidirectionally transported cargos it is an open question whether the characteristics of their motion can result from pure stochastic fluctuations or whether some coordination of the motors is needed. The results of a mean-field model of cargo-motors dynamics, which was proposed by M\"uller et al.[1] suggest the existence of high motility states which would result from a stochastic tug-of-war. Here we analyze a non-mean field extension of their model, that takes explicitly the position of each motor into account. We find that high motility states then disappear. We consider also a mutual motor-motor activation, as an explicit mechanism of motor coordination. We show that the results of the mean-field model are recovered only in case of a strong motor-motor activation in the limit of a high number of motors.Comment: 6 pages, 10 figure

The Asymmetric Exclusion Process revisited: Fluctuations and Dynamics in the Domain Wall Picture

We investigate the total asymmetric exclusion process by analyzing the dynamics of the shock. Within this approach we are able to calculate the fluctuations of the number of particles and density profiles not only in the stationary state but also in the transient regime. We find that the analytical predictions and the simulation results are in excellent agreement.Comment: 6 figures. Submitted to J. Stat. Phy

Crossing pedestrian traffic flows,diagonal stripe pattern, and chevron effect

We study two perpendicular intersecting flows of pedestrians. The latter are represented either by moving hard core particles of two types, eastbound (\symbp) and northbound (\symbm), or by two density fields, \rhop_t(\brr) and \rhom_t(\brr). Each flow takes place on a lattice strip of width $M$ so that the intersection is an $M\times M$ square. We investigate the spontaneous formation, observed experimentally and in simulations, of a diagonal pattern of stripes in which alternatingly one of the two particle types dominates. By a linear stability analysis of the field equations we show how this pattern formation comes about. We focus on the observation, reported recently, that the striped pattern actually consists of chevrons rather than straight lines. We demonstrate that this `chevron effect' occurs both in particle simulations with various different update schemes and in field simulations. We quantify the effect in terms of the chevron angle $\Delta\theta_0$ and determine its dependency on the parameters governing the boundary conditions.Comment: 36 pages, 22 figure

Wake-mediated interaction between driven particles crossing a perpendicular flow

Diagonal or chevron patterns are known to spontaneously emerge at the intersection of two perpendicular flows of self-propelled particles, e.g. pedestrians. The instability responsible for this pattern formation has been studied in previous work in the context of a mean-field approach. Here we investigate the microscopic mechanism yielding to this pattern. We present a lattice model study of the wake created by a particle crossing a perpendicular flow and show how this wake can localize other particles traveling in the same direction as a result of an effective interaction mediated by the perpendicular flow. The use of a semi-deterministic model allows us to characterize analytically the effective interaction between two particles.Comment: 23 pages, 8 figures. To appear in J. Stat. Mec

Zone clearance in an infinite TASEP with a step initial condition

The TASEP is a paradigmatic model of out-of-equilibrium statistical physics, for which many quantities have been computed, either exactly or by approximate methods. In this work we study two new kinds of observables that have some relevance in biological or traffic models. They represent the probability for a given clearance zone of the lattice to be empty (for the first time) at a given time, starting from a step density profile. Exact expressions are obtained for single-time quantities, while more involved history-dependent observables are studied by Monte Carlo simulation, and partially predicted by a phenomenological approach

Properties of pedestrians walking in line: Stepping behavior

In human crowds, interactions among individuals give rise to a variety of self-organized collective motions that help the group to effectively solve the problem of coordination. However, it is still not known exactly how humans adjust their behavior locally, nor what are the direct consequences on the emergent organization. One of the underlying mechanisms of adjusting individual motions is the stepping dynamics. In this paper, we present first quantitative analysis on the stepping behavior in a one-dimensional pedestrian flow studied under controlled laboratory conditions. We find that the step length is proportional to the velocity of the pedestrian, and is directly related to the space available in front of him, while the variations of the step duration are much smaller. This is in contrast with locomotion studies performed on isolated pedestrians and shows that the local density has a direct influence on the stepping characteristics. Furthermore, we study the phenomena of synchronization -walking in lockstep- and show its dependence on flow densities. We show that the synchronization of steps is particularly important at high densities, which has direct impact on the studies of optimizing pedestrians flow in congested situations. However, small synchronization and antisynchronization effects are found also at very low densities, for which no steric constraints exist between successive pedestrians, showing the natural tendency to synchronize according to perceived visual signals.Comment: 8 pages, 5 figure