Effect of propolis gel on the in vitro reduction of dentin permeability

OBJECTIVE: The aim of this study was to evaluate the capacity of potassium oxalate, fluoride gel and two kinds of propolis gel to reduce the hydraulic conductance of dentin, in vitro. MATERIAL AND METHODS: The methodology used for the measurement of hydraulic conductance of dentin in the present study was based on a model proposed in literature. Thirty-six 1-mm-thick dentin discs, obtained from extracted human third molars were divided into 4 groups (n=9). The groups corresponded to the following experimental materials: GI-10% propolis gel, pH 4.1; GII-30% propolis gel; GIII-3% potassium oxalate gel, pH 4,1; and GIV-1.23% fluoride gel, pH 4.1, applied to the dentin under the following surface conditions: after 37% phosphoric acid and before 6% citric acid application. The occluding capacity of the dentin tubules was evaluated using scanning electron microscopy (SEM) at ×500, ×1,000 and ×2,000 magnifications. Data were analyzed statistically by two-way ANOVA and Tukey's test at 5% significance level. RESULTS: Groups I, II, III, IV did not differ significantly from the others in any conditions by reducing in hydraulic conductance. The active agents reduced dentin permeability; however they produced the smallest reduction in hydraulic conductance when compared to the presence of smear layer (P<0.05). The effectiveness in reducing dentin permeability did not differ significantly from 10% or 30% propolis gels. SEM micrographs revealed that dentin tubules were partially occluded after treatment with propolis. CONCLUSIONS: Under the conditions of this study, the application of 10% and 30% propolis gels did not seem to reduce the hydraulic conductance of dentin in vitro, but it showed capacity of partially obliterating the dentin tubules. Propolis is used in the treatment of different oral problems without causing significant great collateral effects, and can be a good option in the treatment of patients with dentin sensitivity

MiR-128 Inhibits Tumor Growth and Angiogenesis by Targeting p70S6K1

MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-128 expression levels were decreased in glioma, and identified p70S6K1 as a novel direct target of miR-128. Overexpression of miR-128 suppressed p70S6K1 and its downstream signaling molecules such as HIF-1 and VEGF expression, and attenuated cell proliferation, tumor growth and angiogenesis. Forced expression of p70S6K1 can partly rescue the inhibitory effect of miR-128 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-128 in regulating glioma tumor angiogenesis via miR-128/p70S6K1 axis, and miR-128 may serve as a potential therapeutic target in glioma in the future

Epidemiological aspects of american cutaneous leishmaniasis and phlebotomine sandfly population, in the municipality of monte negro, state of Rondônia, Brazil

Introduction: This work was carried out on the purpose of identifying the species of phlebotomine sandflies in the municipality of Monte Negro, state of Rondonia, Brazil, that may have been transmitting the American cutaneous leishmaniasis (ACL), and concisely describe epidemiological aspects of disease. Methods: The epidemiologic and socioeconomical indicators were obtained from government institutions and the local Municipal Secretary of Health. Phlebotomine sandflies were captured using CDC light traps between July 2006 to July 2008. The total of 1,240 of female sandflies were examined by PCR method directed to k-DNA. Results: There has been a significant decrease in the incidence of ACL of about 50% over the last ten years in the municipality. A total of 1,935 specimens of 53 sandfly species were captured, three of the genus Brumptomyia genus and 50 of the genus Lutzomyia. The predominant species was Lutzomyia acanthopharynx, Lutzomyia whitmani, Lutzomyia geniculata and Lutzomyia davisi. None were positive for Leishmania sp. Conclusions: Four sandflies species were found in the State of Rondonia for the first time: Brumptomyia brumpti, Lutzomyia tarapacaensis, Lutzomyia melloi and Lutzomyia lenti. The presence of Lutzomyia longipalpis, was also captured. Socioeconomical improvement of Brazilian economy and the increase of environmental surveillance in the last 15 years collaborated in the decrease of people exposed to vectors, reducing the incidence of ACL

Avaliação da alimentação de idosos de município paulista: aplicação do Índice de Alimentação Saudável

OBJETIVO: avaliar a qualidade da dieta da população idosa do município de Avaré (SP) através do Índice de Alimentação Saudável (IAS) MÉTODOS: trata-se de um estudo de corte transversal de base populacional realizado por meio de entrevista domiciliar. A amostra constou de 73 indivíduos, sorteados aleatoriamente dos idosos integrados ao Sistema Público de Saúde do Município. O consumo alimentar foi medido por meio de 3 Recordatórios de 24 horas. Para avaliação, foi aplicado o IAS adaptado para a população brasileira. Parte-se do princípio que o presente estudo constitui o primeiro no Brasil a aplicar o IAS utilizando 3 inquéritos do tipo recordatório de 24 horas em população idosa. Optou-se por esta metodologia, pois como descrito na literatura, um único dia não representa a ingestão habitual de um indivíduo devido à elevada variabilidade intrapessoal do consumo. RESULTADOS: Foram encontrados 32,9% de idosos com uma dieta de má qualidade; 60,3% necessitando de melhorias e 6,8% com uma dieta de boa qualidade. CONCLUSÃO: Pode-se concluir que os idosos estudados precisam de melhorias na alimentação, o que ressalta a importância de política de incentivo voltado à alimentação saudável na terceira idade.The scope of this paper was to evaluate the quality of the diet of the elderly in the city of Avaré, São Paulo state, using the Healthy Eating Index (HEI). A cross-sectional population-based study was conducted by home interviews. The sample consisted of 73 individuals, randomly selected among elderly people from the public health system in the city. Food consumption was measured by 3 24-hour recalls. The HEI adapted to the Brazilian population was applied for evaluation purposes. It is believed that this study is the first in Brazil to apply the HEI using 3 surveys of the 24-hour recall type among the elderly population. This methodology was chosen because, as described in the literature, a single day does not accurately reflect the usual intake of an individual due to the high interpersonal variance in consumption. It was found that 32.9% of elderly people were on a poor quality diet, 60.3% needed adjustments and 6.8% had a good quality diet. The conclusion that can be drawn is that the elderly population studied need to improve their diet, which emphasizes the importance of policies geared to encouraging healthy eating in old age.Universidade Estadual de São Paul

Anomalisa e o ensino da comunicação clínica na prática médica

Resumo: Partindo de reflexões do filme Anomalisa e apoiando-se no conceito foucaultiano de biopoder, este ensaio discute os limites do ensino de habilidades de comunicação clínica na formação em medicina. A comunicação com pacientes não se relaciona ao uso (ou não-uso) de certas frases ou de determinado estilo de conversa, mas significa a busca de uma maneira de interagir com o outro em meio à desordem do mundo, à arrebatadora onda de sofrimento e ao intervalo de tempo assustador entre a vida e a morte. Nesse cenário em que figuram as tecnobiociências e imperam as evidências científicas, para além dos protocolos ou da padronização dos modos de se praticar a clínica, propõe-se um debate sobre o ensino da comunicação com ênfase na produção de subjetividades, de modo a veicular um chamamento à reflexão sobre valores desejáveis para a atuação no campo da saúde