Promoter sequences from Hevea brasiliensis hevein genes

The present invention relates to the isolation of DNA sequences from the Hevea brasiliensis containing the promoter and regulatory region of hevein genes. The promoter sequences of the hevein genes also act as an inducible promoter regulated by wounds and pathogen infection. (Résumé d'auteur

Synteza nowego koniugatu nanocząsteczek magnetycznych z nanopęcherzykami perfluorowęglowodoru jako potencjalnej terapii wspomagającej w ostrym niedokrwieniu mięśnia sercowego

Introduction. This study was performed in search of a novel adjunct agent for myocardial salvage in acute myocardial ischaemia. Methods and results. This is a report of conjugation between perfluorocarbon nanobubbles with multifunctional biocompatible magnetic nanoparticles. It was possible to synthesise the conjugation of multifunctional biocompatible magnetic nanoparticles with perfluorocarbon nanobubbles. The potential nano-size magnetic product could cross the deranged endothelial borders in the ischaemic penumbra regions, and could help in myocardial salvage. Furthermore, the particles could be focused to the target region using the magnetic micromesh technique. Conclusions. A conjugation between magnetic nanoparticles and PFC nano-bubbles is possible. Further studies need to be performed to explore the potential uses.Wstęp. Celem badania było poszukiwanie nowych leków wspomagających zachowanie żywotności miokardium po ostrymniedokrwieniu mięśnia sercowego. Metody i wyniki. W niniejszej pracy opisano koniugację nanopęcherzyków perfluorowęglowodoru z biokompatybilnymiwielofunkcyjnymi nanocząsteczkami magnetycznymi. Okazało się, że możliwa jest synteza koniugatu wielofunkcyjnychbiokompatybilnych cząstek magnetycznych z nanopęcherzykami perfluorowęglowodoru. Otrzymane w ten sposób nanocząsteczkimagnetyczne mogą przenikać przez uszkodzony śródbłonek w regionie otaczającym obszar zawału i mogłybyzostać wykorzystane do ratowania mięśnia sercowego przed trwałym uszkodzeniem. Ponadto cząsteczki te możnadoprowadzić do docelowego miejsca, stosując technikę mikrosiatki magnetycznej. Wnioski. Koniugacja między nanocząsteczkami magnetycznymi a nanopęcherzykami perfluorowęglowodoru jest możliwa.Należy przeprowadzić dalsze badania nad możliwościami ich wykorzystania

Analysis of the Influence of Skills on Playing Ability among Junior Soccer Players

The purpose of the present investigation was to analyse the relationship between the selected skills with the playing ability and the influence of skills on the playing ability among the soccer players of defending playing position. Method: 30 male soccer players (defenders) from Puducherry Union Territory, India, age ranged between 17 and 19 years were selected by random sampling technique(n=30). To assess the playing ability of the subjects, the rating technique was followed by using three experts. The skill performance (short pass, long pass, foot receiving and passing, thigh receiving and passing, chest receiving and passing, Defensive heading, attacking heading, dribbling and shooting) was quantified by adopting appropriate tests. The test reliability was established by the investigator by test retest method for 10 soccer players. Result: Among the selected skills, short pass (0.468, p< 0.05), thigh receiving and passing (0.508, p<0.05), chest receiving and passing (0.608, p<0.01), attacking heading (0.482, p< 0.05), defensive heading (0.755, p<0.01) and shooting (0.666, p<0.01) had positive significant relationship with the soccer playing ability. The 90% of playing ability of defenders is mainly due to the skills such as defensive heading (57), attacking heading (15%), long pass (11%) and shooting (7%)

Cytokine Response of CD4+ T-Lymphocytes with Red Rose (Rosa Rosaceae – Pierre de Ronsard) Extracts by in Vitro Evaluation

Background. Red rose extract is known to have anti-inflammatory and immune-modulation effects. In this study, the red rose extract was tested on CD4+T lymphocytes in vitro, and cytokine response was evaluated. Materials and Methods. The red rose (Rosa Rosaceae - Pierre de Ronsard) extract used in this study was prepared and stored at -20° C until use. CD4+T-cells were seeded in 96-well plates at 313,500 cells/well in 100μ l cell culture medium in duplicate. One-half of the wells were used for biomarker screening in the culture medium, and the other half was used for cytotoxicity assay. Twenty-four hours after plating, the cells were treated in duplicate with 100μ l of the red rose extract diluted at 0.5%, 0.1%, 0.05%, 0.01% and 0.005% (v/v) in the cell culture medium or with culture medium only as control for 72 hours. Some other wells were allocated for untreated cells, and cells treated with the rose extract at 0.005% for 48-h incubation time. Results. Several cytokines (GRO; IFN-γ; IL-1α, 6, 10; MCP-1; RANTES; TGF-β1; TIMP 1, 2; Ang1, Ang2; G-CSF; MMP-9; and VEGF R2) were elevated. Except for MMP-9, which had fold changes > 2, other cytokines were minimally elevated at various concentrations and timing of rose extract treatment. None of the mentioned cytokines were less than 0.8-fold after treatment with the rose extract. Cytotoxicity assay revealed insignificant changes in the viability of T-cells. Conclusions. There was a mild elevation in few inflammatory markers by CD4+ T-lymphocytes after in vitro treatment with the red rose extract (Rosa Rosacea - Pierre De Ronsard). Further in vitro and in vivo studies are required to evaluate the benefits of the red rose extract in immune regulation

Evaluation of Magnetic Parameters and Kinetics of the Magnetic Nanoparticles in High Magnetic Fields and its Potential Applications

BACKGROUND: Multifunctional nanoparticles are known for their wide range of biomedical applications. Controlling the magnetic properties of these nanoparticles is imperative for various applications, including therapeutic angiogenesis. AIM: The study was performed to evaluate the magnetic properties and their control mechanisms by the external magnetic field. METHODS: A100 nm magnetic nanoparticle was placed in the magnetic field, and parametrically, the magnet field strength and distance were evaluated. Various models of magnetic strength and disposition were evaluated. Magnetic flux density, force/weight, and magnetic gradient strength were the parameters evaluated in the electromagnetic computational software. RESULTS: The seven-coil method with three centrally placed coils as Halbach array, and each coil with a flux density of 7 Tesla, and with a coil dimension of 20 cm × 20 cm (square model) of each coil showed a good magnetic strength and force/weight parameters in a distance of 15 cm from the centrally placed coil. The particles were then evaluated for their motion characteristics in saline. It showed good displacement and acceleration properties. After that, the particles were theoretically assessed in a similar mathematical model after parametrically correcting the drag force. After the application of high drag forces, the particles showed adequate motion characteristics. When the particle size was reduced further, the motion characteristics were preserved even with high drag forces. CONCLUSION: There is potential for a novel method of controlling multifunctional magnetic nanoparticles using high magnetic fields. Further studies are required to evaluate the motion characteristics of these particles in vivo and in vitro

Determination of Hospitality Services Quality and Customer Satisfaction – a Holserv Approach

This study examines the relationship between quality of perceived service and level of satisfaction customer attained in Tourism. Although the aforesaid relationship discussions proliferated, studies have employed customised SERVQUL for tourism remains dearth. This research attempts to fulfil the gap by using HOLSERV measurement instrument. HOLSERV is developed to measure the service quality in tourism, comprised with three factors named employee, tangibles, and reliability. Data have been collected from guests of the luxury hotels located in Pondicherry (Puducherry). 344 samples were used for data analyses after eliminating the unusable data by preliminary psychometric test. Confirmatory Factor Analysis (CFA) followed by Structural Equation Modelling (SEM) was employed to examine the postulated relationship. The results supported the proposed hypotheses. Employees, tangibles, and reliability are confirmed as factors of hospitality service quality by CFA. Employee factor ascertained to be an important factor by SEM. From the results and findings of this study, implications have been drawn, limitations were given, and recommendations were made for future researcher

Impact of Food Safety and Standards Regulation on Food Business Operators

The main aim of the study is to understand food regulation and food safety in India context and global aspects. It also explains the major health problem and recent past reports of FSSAI. The primary data were collected from food business operators (mobile food vendors, many small scale hotels, and local restaurants). It also helps to ensure the knowledge and awareness level of the food business operators. This study is to understand the view of food-business operators in a B-grade city of India “Salem” in Tamil Nadu about the FSSAI regulations and the status of performance of the food safety department in maintaining safety and quality of food and also to know about the level of awareness among the usage of fortified foods among food-businesses. The Study concludes that the Food and Safety Officers should regularly visit for inspection and monitor the food business operators for the betterment of public health. There should be proper awareness about the ingredients are used in fortified food among food-businesses to eliminate malnutrition and avoid food adulteration. The future rules and regulations of FSSAI should be strictly implementing all over India and properly monitor their activities of food business operators to fulfil the FSSAI standards

Analysis of Urban Population Growth Pattern for Chennai – Zone IX, TEYNAMPET

Graph-cellular automata (GCA) is implemented for zone IX of Chennai city. The population, population density, educational institution, industries, etc were taken as attribute. It is shown that GCA with particular structural properties defined in terms of the relationships between subsets of cells is a useful generalization of traditional cellular automaton (CA). The population data from Chennai census 1981, 1991, and 2001 were used to develop the GCA and the results are enumerated. The obtained results are compared with census data 2011

Angioplasty with Stenting in Acute Coronary Syndromes with Very Low Contrast Volume Using 6F Diagnostic Catheters and Bench Testing of Catheters

AIM: To safely perform angioplasties in acute coronary syndromes with low contrast volume using Cordis 6F diagnostic catheters and to perform mechanical bench tests on the diagnostic and guide catheters in a radial path model. METHODS: In 191 patients (242 lesions/268 stents) with acute coronary syndromes angioplasty were performed with cordis 6F diagnostic catheters. RESULTS: The lesions were present at left anterior descending (121), Left main (5), left circumflex (51), ramus (5) and right coronary artery (60). In 72% of cases, Iodixanol was used. All contrast injections were given by hand. Regular follow-up of the patients was performed at 30 days. The procedures were performed in the femoral route only. Pre-dilatation was performed in 43 cases. Successful revascularization of the target lesion was achieved in all cases. The mean contrast volume used per patient was 28 ml (± 8 ml). Mild reversible contrast-induced nephropathy (CIN) was observed in two patients. Cardiogenic shock was seen in 7 cases, and one death was observed. Pushability and trackability tests showed good force transmission and hysteresis in diagnostic catheters compared to guide catheters. CONCLUSIONS: Angioplasty with stenting could be performed safely in patients using cordis 6F diagnostic catheters using a low volume of contrast in acute coronary syndromes. Low contrast volume usage would result in a lower incidence of contrast-induced nephropathy and cardiac failures