Model for the high cycle corrosion-fatigue damage of machine parts

The report is dedicated to a problem of the phenomenological specification statement of corrosive fatigue damage in the small stresses conditions. As the baseline equation usage the E.K.Pochtenny’s equation is offered, which one will to the greatest degree be matched with experimental results in a high-cycle corrosion fatigue conditions for steel specimens and full-scale parts. Also this equation is in a basis of the whole complex of designed methods of an estimation of durability and residual operational life of accountable of members of constructions operating in high-cycle fatigue conditions. The disadvantage of the equation is asymptotically lower branch, coming nearer to endurance strength, of a curve of fatigue, which one in corrosive conditions does not correspond to results of experiments. For overcoming this disadvantage the two-parameter equation for lower branches of a curve of a corrosion fatigue is offered. The calculus of arguments is spent with the help of the system of the nonlinear equations. The result of applying of the equation is showed on an actual example of a pump rods curve of fatigue

Molecular Proximity of Kv1.3 Voltage-gated Potassium Channels and β1-Integrins on the Plasma Membrane of Melanoma Cells: Effects of Cell Adherence and Channel Blockers

Tumor cell membranes have multiple components that participate in the process of metastasis. The present study investigates the physical association of β1-integrins and Kv1.3 voltage-gated potassium channels in melanoma cell membranes using resonance energy transfer (RET) techniques. RET between donor-labeled anti–β1-integrin and acceptor-labeled anti-Kv1.3 channels was detected on LOX cells adherent to glass and fibronectin-coated coverslips. However, RET was not observed on LOX cells in suspension, indicating that molecular proximity of these membrane molecules is adherence-related. Several K+ channel blockers, including tetraethylammonium, 4-aminopyridine, and verapamil, inhibited RET between β1-integrins and Kv1.3 channels. However, the irrelevant K+ channel blocker apamin had no effect on RET between β1-integrins and Kv1.3 channels. Based on these findings, we speculate that the lateral association of Kv1.3 channels with β1-integrins contributes to the regulation of integrin function and that channel blockers might affect tumor cell behavior by influencing the assembly of supramolecular structures containing integrins