Pembuatan Aplikasi Tata Ruang Tiga Dimensi Gedung Serba Guna Menggunakan Teknologi Virtual Reality [Studi Kasus : Graha ITS Surabaya]

Pada bidang pemasaran dan periklanan di Perusahaan saat ini cenderung menampilkan Gambar 2D dalam penyampaian informasi kepada pengguna. Gambar 2D yang sering kita tahu berfungsi sebagai media penyampaian informasi. Misal informasi tentang suatu tata ruang, namun informasi yang dapat disajikan hanya dari sisi-sisi tertentu saja. Untuk itu, dibutuhkan tampilan visual yang dapat mengGambarkan tata ruang suatu bangunan dalam bentuk 3D. Sehingga seseorang dapat mengetahui informasi suatu ruangan dari berbagai sisi. Karena teknologi semakin canggih, diharapkan aplikasi yang dibuat dapat dinikmati semua kalangan. Pada pembuatan tugas akhir, penulis membuat tata ruang gedung yang dapat ditampilkan dalam bentuk 3D menggunakan teknologi 3D yaitu virtual reality. Virtual reality merupakan ruang digital dimana data input sudah diprogram sebelumnya. Pada pengerjaan tugas akhir ini menggunakan tool augmented reality adalah penggabungan antara objek-objek di dunia nyata dan dunia maya yang berjalan secara interaktif karena saling terintegrasi. Dengan memberikan input berupa marker maka output yang dihasilkan berupa tampilan 3D yang sesuai. Lokasi gedung yang dibuat yaitu Gedung Graha ITS. Penulis mengharapkan, aplikasi dapat digunakan untuk menunjukkan tata ruang dan contoh dekorasi kegiatan di Gedung Graha ITS secara akurat dan interaktif

Characterization of At- species in simple and biological media by high performance anion exchange chromatography coupled to gamma detector.

Astatine is a rare radioelement belonging to the halogen group. Considering the trace amounts of astatine produced in cyclotrons, its chemistry cannot be evaluated by spectroscopic tools. Analytical tools, provided that they are coupled with a radioactive detection system, may be an alternative way to study its chemistry. In this research work, High Performance Anion Exchange Chromatography (HPAEC) coupled to a gamma detector (γ) was used to evaluate astatine species under reducing conditions. Also, to strengthen the reliability of the experiments, a quantitative analysis using a reactive transport model has been done. The results confirm the existence of one species bearing one negative charge in the pH range 27.5. With respect to the other halogens, its behavior indicates the existence of negative ion, astatide At-. The methodology was successfully applied to the speciation of the astatine in human serum. Under fixed experimental conditions (pH 7.47.5 and redox potential of 250 mV) astatine exists mainly as astatide At- and does not interact with the major serum components. Also, the method might be useful for the in vitro stability assessment of 211At-labelled molecules potentially applicable in nuclear medicine

Synthesis of macrocyclic receptors with intrinsic fluorescence featuring quinizarin moieties

An unprecedented class of macrocycles with intrinsic fluorescence consisting of phenolic trimers and quinizarin is developed. Though they are lacking strong hydrogen bonds as observed in calixarenes, the two examples introduced here each adopt a vase-like conformation with all four aromatic units pointing in one direction (syn orientation). This “cone” conformation has been confirmed by NMR spectroscopy, molecular modeling, and X-ray crystallography. The laminar, electron-rich fluorophore as part of the macrocycle allows additional contacts to enclosed guest molecules

Rapid and Efficient Removal of Perfluorooctanoic Acid from Water with Fluorine-Rich Calixarene-Based Porous Polymers

On account of its nonbiodegradable nature and persistence in the environment, perfluorooctanoic acid (PFOA) accumulates in water resources and poses serious environmental issues in many parts of the world. Here, we present the development of two fluorine-rich calix[4]arene-based porous polymers, FCX4-P and FCX4-BP, and demonstrate their utility for the efficient removal of PFOA from water. These materials featured Brunauer–Emmett–Teller (BET) surface areas of up to 450 m^{2} g^{-1}, which is slightly lower than their nonfluorinated counterparts (up to 596 m^{2} g^{-1}). FCX4-P removes PFOA at environmentally relevant concentrations with a high rate constant of 3.80 g mg^{-1} h^{-1} and reached an exceptional maximum PFOA uptake capacity of 188.7 mg g^{-1}. In addition, it could be regenerated by simple methanol wash and reused without a significant decrease in performance

PENINGKATAN KETERAMPILAN PRODUKSI DAN PEMASARAN DIGITAL PRODUK DAUR ULANG SAMPAH HIDROPONIK DENGAN MESIN CETAK PELET HYBRID TENAGA SURYA

Abstrak: Pengelolaan sampah di Indonesia merupakan tantangan bersama, tingginya volume sampah disebabkan oleh pertumbuhan penduduk dan perubahan hidup masyarakat. Pentingnya kesadaran manusia dalam mengelola sampah, tidak hanya berdampak pada kebersihan lingkungan, namun juga meningkatkan factor ekonomi. Kegiatan ini bertujuan meningkatkan hardskill mitra dalam mendaur ulang sampah menjadi produk bernilai jual berupa pakan hewan, serta mendukung sofskill dalam pemanfaatan media digital. Metode yang digunakan seperti wawancara, praktik pengelolaan sampah, FGD bersama terkait strategi pemasaran. Kegiatan ini merupakan kolaborasi dua mitra yang merambah di bidang hidroponik dan agribisnis, dengan total peserta yang hadir 8 orang selaku pengurus utama. Hasil dari kegiatan ini menunjukkn adanya peningkatan hardskill 80,8% dan softskill 79,6%. Inisiatif ini sejalan dengan Tujuan Pembangunan Berkelanjutan (SDGs) dengan mengintegrasikan pertumbuhan ekonomi berbasis digital dengan keberlanjutan lingkungan.Abstract: Waste management in Indonesia is a shared challenge, with the high volume of waste driven by population growth and changes in community lifestyles. Raising awareness about waste management is crucial, not only for environmental cleanliness but also for enhancing economic factors. This activity aims to improve partners' hardskills in recycling waste into marketable products, such as animal feed, while also enhancing their softskills in utilizing digital media. Methods used include interviews, waste management practices, and Focus Group Discussions (FGDs) on marketing strategies. This initiative is a collaboration between two partners operating in hydroponics and agribusiness, involving a total of 8 participants as key administrators. The results show an improvement in hardskills by 80.8% and softskills by 79.6%. This effort aligns with the Sustainable Development Goals (SDGs) by integrating digital-based economic growth with environmental sustainability

PTPN2, a Candidate Gene for Type 1 Diabetes, Modulates Interferon-γ–Induced Pancreatic β-Cell Apoptosis

OBJECTIVE: The pathogenesis of type 1 diabetes has a strong genetic component. Genome-wide association scans recently identified novel susceptibility genes including the phosphatases PTPN22 and PTPN2. We hypothesized that PTPN2 plays a direct role in beta-cell demise and assessed PTPN2 expression in human islets and rat primary and clonal beta-cells, besides evaluating its role in cytokine-induced signaling and beta-cell apoptosis. RESEARCH DESIGN AND METHODS: PTPN2 mRNA and protein expression was evaluated by real-time PCR and Western blot. Small interfering (si)RNAs were used to inhibit the expression of PTPN2 and downstream STAT1 in beta-cells, allowing the assessment of cell death after cytokine treatment. RESULTS: PTPN2 mRNA and protein are expressed in human islets and rat beta-cells and upregulated by cytokines. Transfection with PTPN2 siRNAs inhibited basal- and cytokine-induced PTPN2 expression in rat beta-cells and dispersed human islets cells. Decreased PTPN2 expression exacerbated interleukin (IL)-1beta + interferon (IFN)-gamma-induced beta-cell apoptosis and turned IFN-gamma alone into a proapoptotic signal. Inhibition of PTPN2 amplified IFN-gamma-induced STAT1 phosphorylation, whereas double knockdown of both PTPN2 and STAT1 protected beta-cells against cytokine-induced apoptosis, suggesting that STAT1 hyperactivation is responsible for the aggravation of cytokine-induced beta-cell death in PTPN2-deficient cells. CONCLUSIONS: We identified a functional role for the type 1 diabetes candidate gene PTPN2 in modulating IFN-gamma signal transduction at the beta-cell level. PTPN2 regulates cytokine-induced apoptosis and may thereby contribute to the pathogenesis of type 1 diabetes

Lessons From the First Comprehensive Molecular Characterization of Cell Cycle Control in Rodent Insulinoma Cell Lines

OBJECTIVE—Rodent insulinoma cell lines may serve as a model for designing continuously replicating human β-cell lines and provide clues as to the central cell cycle regulatory molecules in the β-cell

A New Strategy to Generate Functional Insulin-Producing Cell Lines by Somatic Gene Transfer into Pancreatic Progenitors

BACKGROUND: There is increasing interest in developing human cell lines to be used to better understand cell biology, but also for drug screening, toxicology analysis and future cell therapy. In the endocrine pancreatic field, functional human beta cell lines are extremely scarce. On the other hand, rodent insulin producing beta cells have been generated during the past years with great success. Many of such cell lines were produced by using transgenic mice expressing SV40T antigen under the control of the insulin promoter, an approach clearly inadequate in human. Our objective was to develop and validate in rodent an alternative transgenic-like approach, applicable to human tissue, by performing somatic gene transfer into pancreatic progenitors that will develop into beta cells. METHODS AND FINDINGS: In this study, rat embryonic pancreases were transduced with recombinant lentiviral vector expressing the SV40T antigen under the control of the insulin promoter. Transduced tissues were next transplanted under the kidney capsule of immuno-incompetent mice allowing insulinoma development from which beta cell lines were established. Gene expression profile, insulin content and glucose dependent secretion, normalization of glycemia upon transplantation into diabetic mice validated the approach to generate beta cell lines. CONCLUSIONS: Somatic gene transfer into pancreatic progenitors represents an alternative strategy to generate functional beta cell lines in rodent. Moreover, this approach can be generalized to derive cells lines from various tissues and most importantly from tissues of human origin

JNK3 Maintains Expression of the Insulin Receptor Substrate 2 (IRS2) in Insulin-Secreting Cells: Functional Consequences for Insulin Signaling

We have recently shown that silencing of the brain/islet specific c-Jun N-terminal Kinase3 (JNK3) isoform enhances both basal and cytokine-induced beta-cell apoptosis, whereas silencing of JNK1 or JNK2 has opposite effects. While it is known that JNK1 or JNK2 may promote apoptosis by inhibiting the activity of the pro-survival Akt pathway, the effect of JNK3 on Akt has not been documented. This study aims to determine the involvement of individual JNKs and specifically JNK3 in the regulation of the Akt signaling pathway in insulin-secreting cells. JNK3 silencing strongly decreases Insulin Receptor Substrate 2 (IRS2) protein expression, and blocks Akt2 but not Akt1 activation by insulin, while the silencing of JNK1 or JNK2 activates both Akt1 and Akt2. Concomitantly, the silencing of JNK1 or JNK2, but not of JNK3, potently phosphorylates the glycogen synthase kinase3 (GSK3β). JNK3 silencing also decreases the activity of the transcription factor Forkhead BoxO3A (FoxO3A) that is known to control IRS2 expression, in addition to increasing c-Jun levels that are known to inhibit insulin gene expression. In conclusion, we propose that JNK1/2 on one hand and JNK3 on the other hand, have opposite effects on insulin-signaling in insulin-secreting cells; JNK3 protects beta-cells from apoptosis and dysfunction mainly through maintenance of a normal IRS2 to Akt2 signaling pathway. It seems that JNK3 mediates its effects mainly at the transcriptional level, while JNK1 or JNK2 appear to mediate their pro-apoptotic effect in the cytoplasm

Nucleophilic Functionalization of the Calix[6]arene Para- and Meta-Position via p‑Bromodienone Route

It is here demonstrated that the p-bromodienone route, previously reported for calix[4]arenes, is also effective for the functionalization of the calix[6]arene macrocycle. Thus, alcoholic O-nucleophiles can be introduced at the calix[6]arene exo rim. In addition, the reaction of a calix[6]arene p-bromodienone derivative with an actived aromatic substrate, such as resorcinol, led to the first example of a meta-functionalized, inherently chiral calix[6]arene derivativ