Ethanol Production from Non Food Tubers of Iles-iles (Amorphophallus campanulatus) using Hydrolyzes by Commercial Enzymes (α and β amylase) and Fermentation by Saccharomices cereviseae

The decrease of oil production caused the increase on the price of fossil fuels. This paper was investigated the possibility of Amorphophallus campanulatus or known as âiles-ilesâ by Javanese people, which is known have a high carbohydrate content, as a raw material to produce bioethanol. The first stage of the process was hydrolyzes the starch, combined by liquefaction and saccharification of the starch from âiles-ilesâ using α and β amylase. The process was followed by fermentation of glucose with the help of S. cerevisiae. To obtain the maximum ethanol content, several parameter had been studied, such as the type S. cerevisiae (pure, dry, wet and instant), the dosage of α-amylase, β-amylase and also DAP dosage as a nutrient support for S. cerevisiae. The result shows that the highest ethanol concentration obtained in fermentation using dry S. cereviseae for 72 h with 10.2% (v/v) of ethanol. The highest total sugar content by hydrolysis was achieved by 0.0032 mL α-amylase/g, while β-amylase was 0.0064 mL β-amylase/g (12.5% of glucose). This is show that with increasing of α and β amylase dosages, the total sugar formed was increased. The DAP (Diammonium phosphate) was used as a Nitrogen supply which is needed by S. cerevisiae to growth and as a results can increase the level of ethanol produced. The additional of DAP in the fermentation prove that it can enhance 8.45% (v/v) of ethanol. Therefore, it can be concluded that the highest levels of ethanol with conventional methods of âiles-ilesâ was obtained at 72 h using the dry S. cerevisiae, with 0.0032 and 0.0064 mL enzyme/g of α and β amylase, respectively. This result shows that the plant seems to be a potential raw material for bioethanol

Solitary Esophageal Varix Simulating a Neoplasm

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73070/1/j.1440-1673.1988.tb02785.x.pd

A profile of hospital-admitted paediatric burns patients in South Africa

<p>Abstract</p> <p>Background</p> <p>Injuries and deaths from burns are a serious, yet preventable health problem globally. This paper describes burns in a cohort of children admitted to the Red Cross Children's Hospital, in Cape Town, South Africa.</p> <p>This six month retrospective case note review looked at a sample of consecutively admitted patients from the 1 ^stApril 2007 to the 30 ^thSeptember 2007. Information was collected using a project-specific data capture sheet. Descriptive statistics (percentages, medians, means and standard deviations) were calculated, and data was compared between age groups. Spearman's correlation co-efficient was employed to look at the association between the total body surface area and the length of stay in hospital.</p> <p>Findings</p> <p>During the study period, 294 children were admitted (f= 115 (39.1%), m= 179 (60.9%)). Hot liquids caused 83.0% of the burns and 36.0% of these occurred in children aged two years or younger. Children over the age of five were equally susceptible to hot liquid burns, but the mechanism differed from that which caused burns in the younger child.</p> <p>Conclusion</p> <p>In South Africa, most hospitalised burnt children came from informal settlements where home safety is a low priority. Black babies and toddlers are most at risk for sustaining severe burns when their environment is disorganized with respect to safety. Burns injuries can be prevented by improving the home environment and socio-economic living conditions through the health, social welfare, education and housing departments.</p

Production of human metabolites of the anti-cancer drug flutamide via biotransformation in Cunninghamella species

Fungi belonging to the genus Cunninghamella have enzymes similar to those employed by mammals for the detoxification of xenobiotics, thus they are useful as models of mammalian drug metabolism, and as a source for drug metabolites. We report the transformation of the anti-cancer drug flutamide in Cunninghamella sp. The most predominant phase I metabolites present in the plasma of humans, 2-hydroxyflutamide and 4-nitro-3-(trifluoromethyl)aniline, were also produced in Cunninghamella cultures. Other phase I and phase II metabolites were also detected using a combination of HPLC, GC–MS and 19F-NMR.SB. 30/4/201