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Eesti Arst 2017; 96(1):

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Eesti Arst 2015; 94(1):

Time-Varying Spectral Modelling of the Solo Violin Tone

The analysis of the spectrum of a single violin tone, to better understand how the various partial components contribute to the sound produced, is undertaken. The analysis involves determining which partials are present and how these partials evolve with respect to time. The short-time Fourier transform is used to implement a solution for the time varying spectra by slicing the sound into short segments called windows and analysing each segment sequentially. A digital signal processing software was used in both the analysis and resynthesis stages of this research. Parameters extracted through analysis are used for resynthesis purposes. Results indicate that spectrum changes over time contribute significantly to the timbre of the violin tone. Aslight shifting of the fundamental frequency was also observed in the sound spectrum of all the sub-sections of the waveform, although this shifting was most marked in the attack and release portions of the ADSR envelope. The results also showed that the intensity of the fundamental harmonic was weaker in the initial attack stage, only dominating when the timbre of the tone stabilised. Within the release portion, inharmonic overtones were shown to occur in the upper partials of the sound spectrum. Finally, the resynthesis process reduces the required hard disk capacity by about 93.8% compared with the sampled waveform, while at the same time producing an audible tone almost indistinguishable from the original

HIV-infektsioon Eestis

HIV-infektsiooni diagnostikat Eestis alustati 1987. aastal. Infektsiooni kulu võib jaotada epideemiaeelseks ja epideemiaperioodiks. Kuni 2000. aastani oli Eesti HIV-infektsiooni väikse esinemissagedusega riik. 2000. aastast olukord muutus seoses nakkuse epideemilise levikuga noorte süstivate narkomaanide hulgas. 2005. aastaks on kokku avastatud üle 4500 HIV-positiivse isiku. Nakkus on väljumas narkomaanide ringist, sageneb heteroseksuaalsete nakatumisjuhtude arv, probleemiks on muutunud HIV-positiivsed rasedad. Artiklis on antud ülevaade HIV-infektsiooni levimusest Eestis aastatel 1987–2004 Tallinna Merimetsa Haigla (2001. aastast alates AS Lääne-Tallinna Keskhaigla nakkuskeskus) ja HIV-nakkuse referentslaboratooriumi andmete alusel. Eesti Arst 2005; 84 (4): 244-24

The Nuclear Matrix Protein, NRP/B, Acts as a Transcriptional Repressor of E2F-mediated Transcriptional Activity

Background: NRP/B, a family member of the BTB/Kelch repeat proteins, is implicated in neuronal and cancer development, as well as the regulation of oxidative stress responses in breast and brain cancer. Our previous studies indicate that the NRP/B-BTB/POZ domain is involved in the dimerization of NRP/B and in a complex formation with the tumor suppressor, retinoblastoma protein. Although much evidence supports the potential role of NRP/B as a tumor suppressor, the molecular mechanisms of NRP/B action on E2F transcription factors have not been elucidated. Methods: Three-dimensional modeling of NRP/B was used to generate point mutations in the BTB/Kelch domains. Tet-on inducible NRP/B expression was established. The NRP/B deficient breast cancer cell line, MDA-MB-231, was generated using lentiviral shNRP/B to evaluate the effect of NRP/B on cell proliferation, invasion and migration. Immunoprecipitation was performed to verify the interaction of NRP/B with E2F and histone deacetylase (HDAC-1), and the expression level of NRP/B protein was analyzed by Western blot analysis. Changes in cell cycle were determined by flow cytometry. Transcriptional activities of E2F transcription factors were measured by chloramphenicol acetyltransferase (CAT) activity. Results: Ectopic overexpression of NRP/B demonstrated that the NRP/B-BTB/POZ domain plays a critical role in E2F-mediated transcriptional activity. Point mutations within the BTB/POZ domain restored E2-promoter activity inhibited by NRP/B. Loss of NRP/B enhanced the proliferation and migration of breast cancer cells. Endogenous NRP/B interacted with E2F and HDAC1. Treatement with an HDAC inhibitor, trichostatin A (TSA), abolished the NRP/B-mediated suppression of E2-promoter activity. Gain or loss of NRP/B in HeLa cells confirmed the transcriptional repressive capability of NRP/B on the E2F target genes, Cyclin E and HsORC (Homo sapiens Origin Recognition Complex). Conclusions: The present study shows that NRP/B acts as a transcriptional repressor by interacting with the co-repressors, HDAC1, providing new insight into the molecular mechanisms of NRP/B on tumor suppression

Ground Stop Analysis

This study intends to analyze a selection of flight data from 2021 and 2022 from UND Aerospace to determine how much time is spent in stopped on the ground, with the engines running, prior to takeoff, during training flights. This study also hopes to explore some of the factors that may cause extended ground stop times and determine if there are any areas that warrant further study in the future

The New Creation according to Is. 65

Behold, I create new heavens and a new earth; and the former shall not be remembered nor come into mind, Is. 65, 17. Of what kind of creation and of what time is the Lord speaking here? Does the new creation mean the Christian Church, which began when our Savior established the New Covenant through His redemptive work and the pouring out of the Holy Ghost, so that the new creation is a specific name for the New Testament Messianic kingdom? Or does it denote a certain period of Christ’s kingdom here on earth, the so-called millennium, a period of one thousand years of glory? Or does the Lord in the words quoted above give a promise of what He will do after this present world has been destroyed? Each of these three views has its adherents in the Church. Which of them is correct