Young@Heart Chorus

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Peroxisome Proliferator-Activated Receptor alpha (PPAR alpha) down-regulation in cystic fibrosis lymphocytes

Background: PPARs exhibit anti-inflammatory capacities and are potential modulators of the inflammatory response. We hypothesized that their expression and/or function may be altered in cystic fibrosis (CF), a disorder characterized by an excessive host inflammatory response. Methods: PPARα, β and γ mRNA levels were measured in peripheral blood cells of CF patients and healthy subjects via RT-PCR. PPARα protein expression and subcellular localization was determined via western blot and immunofluorescence, respectively. The activity of PPARα was analyzed by gel shift assay. Results: In lymphocytes, the expression of PPARα mRNA, but not of PPARβ, was reduced (-37%; p < 0.002) in CF patients compared with healthy persons and was therefore further analyzed. A similar reduction of PPARα was observed at protein level (-26%; p < 0.05). The transcription factor was mainly expressed in the cytosol of lymphocytes, with low expression in the nucleus. Moreover, DNA binding activity of the transcription factor was 36% less in lymphocytes of patients (p < 0.01). For PPARα and PPARβ mRNA expression in monocytes and neutrophils, no significant differences were observed between CF patients and healthy persons. In all cells, PPARγ mRNA levels were below the detection limit. Conclusion: Lymphocytes are important regulators of the inflammatory response by releasing cytokines and antibodies. The diminished lymphocytic expression and activity of PPARα may therefore contribute to the inflammatory processes that are observed in CF

A DSP approach to speech recognition using a PC

Several methods of speech recognition utilizing digital signal processing (DSP) have evolved from research in the field. The general approach is the same in most cases. The speech signal is digitized, processed, and then analyzed for pattern matching to arrive at a recognition decision. The methods are based on rather simple models of speech production, since more is known about how we produce speech than on how we understand speech. This project discusses a popular method of digital speech processing using linear predictive coding, and the model associated with it. Then a speech recognition application of this method based on non-linear time alignment with a log-likelihood measurement is considered. The details of a speech recognition system consisting of a personal computer (PC), data acquisition card, and custom software and hardware are given. Results from experiments with the speech recognition system and conclusions are subsequently presented. The speech recognition system did not perform as expected. In a test case of three word choices the correct word was not selected by the speech recognition system. After several experiments were conducted it was concluded that the hardware was functioning properly, but subtle and difficult to detect errors remained in the system design, that increases in speed are possible and advantageous, and that better algorithms will provide better performance.Bibliography: pages 88-89California State University, Northridge. Department of Engineering

America's Invisible Children: Latino Youth and the Failure of Justice

Examines the justice system's treatment of Hispanic/Latino youth, including the large number held in adult jails and prisons, and makes policy recommendations for reforms. Discusses promising practices in community-based alternatives to incarceration

Thoracic organ transplantation

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106665/1/j.1600-6135.2004.00401.x.pd

Series Resistance Analysis of Passivated Emitter Rear Contact Cells Patterned Using Inkjet Printing

For higher-efficiency solar cell structures, such as the Passivated Emitter Rear Contact (PERC) cells, to be fabricated in a manufacturing environment, potentially low-cost techniques such as inkjet printing and metal plating are desirable. A common problem that is experienced when fabricating PERC cells is low fill factors due to high series resistance. This paper identifies and attempts to quantify sources of series resistance in inkjet-patterned PERC cells that employ electroless or light-induced nickel-plating techniques followed by copper light-induced plating. Photoluminescence imaging is used to determine locations of series resistance losses in these inkjet-patterned and plated PERC cells

Pierre Robin sequence and obstructive sleep apnea

The case of a 12-year-old female patient with Pierre Robin sequence is reported, in which reduction of the pharyngeal airway leads to obstructive sleep apnea syndrome (OSAS) and excessive daytime sleepiness. Radiological evaluation, computerized tomography and magnetic resonance image showed bilateral temporomandibular ankylosis. Cephalometric data evidenced marked reduction of the posterior airway space. Three all-night polysomnographic evaluations detected severe OSAS with decrease in oxygen saturation. The Multiple Sleep Latency Test (MSLT) perfomed on two separate days objectively quantified the excessive daytime sleepiness with short sleep latencies; stage REM was not present. Polysomnography, MSLT and thorough radiologic studies, in this case, made it possible to determine the severity of OSAS, the site of obstruction, and the associated malformations

The e-learning platform of the FP7-SOPHIA Project: obtanied results and perspective for its future exploitation

FP7-SOPHIA, the European PV Research Infrastructure project coordinated by CEA-INES ended on 31st of January 2015. The project focused on strengthening and optimising the research capabilities of outstanding European Research Infrastructures by pulling together numerous scientists and researchers of more than 48 relevant Research Infrastructures to share a common vision and to conduct efficient and coordinated research work in the field of PV technologies. SOPHi@Webinar is the internal e-learning platform that has organized a set of online courses/seminaries/guest lectures in parallel to more conventional training initiatives held physically. It has also been opened to non-SOPHIA members

Anti-Inflammatory Effect of Fluvastatin on IL-8 Production Induced by Pseudomonas aeruginosa and Aspergillus fumigatus Antigens in Cystic Fibrosis

International audienceBACKGROUND: Early in life, patients with cystic fibrosis (CF) are infected with microorganisms including bacteria and fungi, particularly Pseudomonas aeruginosa and Aspergillus fumigatus. Since recent research has identified the anti-inflammatory properties of statins (besides their lipid-lowering effects), we investigated the effect of fluvastatin on the production of the potent neutrophil chemoattractant chemokine, IL-8, in whole blood from CF patients, stimulated by Pseudomonas aeruginosa (LPS) and Aspergillus fumigatus (AFA) antigens. RESULTS: Whole blood from adult patients with CF and from healthy volunteers was collected at the Rennes University Hospital (France). Blood was pretreated for 1 h with fluvastatin (0-300 µM) and incubated for 24 h with LPS (10 µg/mL) and/or AFA (diluted 1/200). IL-8 protein levels, quantified by ELISA, were increased in a concentration-dependent manner when cells were stimulated by LPS or AFA. Fluvastatin strongly decreased the levels of IL-8, in a concentration-dependent manner, in whole blood from CF patients. However, its inhibitory effect was decreased or absent in whole blood from healthy subjects. Furthermore, the inhibition induced by fluvastatin in CF whole blood was reversed in the presence of intermediates within the cholesterol biosynthesis pathway, mevalonate, farnesyl pyprophosphate or geranylgeranyl pyrophosphate that activate small GTPases by isoprenylation. CONCLUSIONS: For the first time, the inhibitory effects of fluvastatin on CF systemic inflammation may reveal the important therapeutic potential of statins in pathological conditions associated with the over-production of pro-inflammatory cytokines and chemokines as observed during the manifestation of CF. The anti-inflammatory effect could be related to the modulation of the prenylation of signalling proteins