492 research outputs found

    Nonlinear Analysis of Rayleigh-Taylor Instability of Cylindrical Flow With Heat and Mass Transfer

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    We study the nonlinear Rayleigh-Taylor instability of the interface between two viscous fluids, when the phases are enclosed between two horizontal cylindrical surfaces coaxial with the interface, and when there is mass and heat transfer across the interface. The fluids are considered to be viscous and incompressible with different kinematic viscosities. The method of multiple expansions has been used for the investigation. In the nonlinear theory, it is shown that the evolution of the amplitude is governed by a Ginzburg-Landau equation. The various stability criteria are discussed both analytically and numerically and stability diagrams are obtained. It has been observed that the heat and mass transfer has stabilizing effect on the stability of the system in the nonlinear analysis

    Study on Electrohydrodynamic Rayleigh-Taylor Instability with Heat and Mass Transfer

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    The linear analysis of Rayleigh-Taylor instability of the interface between two viscous and dielectric fluids in the presence of a tangential electric field has been carried out when there is heat and mass transfer across the interface. In our earlier work, the viscous potential flow analysis of Rayleigh-Taylor instability in presence of tangential electric field was studied. Here, we use another irrotational theory in which the discontinuities in the irrotational tangential velocity and shear stress are eliminated in the global energy balance. Stability criterion is given by critical value of applied electric field as well as critical wave number. Various graphs have been drawn to show the effect of various physical parameters such as electric field, heat transfer coefficient, and vapour fraction on the stability of the system. It has been observed that heat transfer and electric field both have stabilizing effect on the stability of the system.</jats:p

    Recovery of phosphate and dissolved organic matter from aqueous solution using a novel CaO-MgO hybrid carbon composite and its feasibility in phosphorus recycling

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    Metal oxide-Carbon composites have been developed tailoring towards specific functionalities for removing pollutants from contaminated environmental systems. In this study, we synthesized a novel CaO-MgO hybrid carbon composite for removal of phosphate and humate by co-pyrolysis of dolomite and sawdust at various temperatures. Increasing of pyrolysis temperature to 900 °C generated a composite rich in carbon, CaO and MgO particles. Phosphate and humate can be removed efficiently by the synthesized composite with the initial solution in the range of pH 3.0–11.0. The phosphate adsorption was best fitted by pseudo-second-order kinetic model, while the humate adsorption followed the pseudo-second-order and the intra-particle diffusion kinetic models. The maximum adsorption capabilities quantified by the Langmuir isotherm model were up to 207 mg phosphorus (or 621 mg phosphate) and 469 mg humate per one-gram composite used, respectively. Characterization of composites after adsorption revealed the contributions of phosphate crystal deposition and electrostatic attraction on the phosphate uptake and involvement of π − π interaction in the humate adsorption. The prepared composite has great potential for recovering phosphorus from wastewater, and the phosphate sorbed composite can be employed as a promising phosphorus slow-releasing fertilizer for improving plant growth

    In-Vessel Co-Composting of Food Waste Employing Enriched Bacterial Consortium

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    Svrha je ovoga rada bila pripremiti odgovarajuću smjesu za kompostiranje s pomoću kulture bakterija i 2 % vapna za učinkovitu obradu otpada od hrane u posudi zapremnine 60 litara. U pokusu, koji je trajao 42 dana, otpaci su od hrane prvo pomiješani s piljevinom i 2 % (suhe tvari) vapna, zatim je u jedan reaktor dodana obogaćena kultura bakterija, dok se u drugom reaktoru nalazio kontrolni uzorak. Rezultati pokazuju da se inokuliranjem smjese za kompostiranje bakterijskom kulturom može uspješno riješiti problem zasićenosti uljem te poboljšati mineralizacija. Osim toga, parametri kao što su: emisija ugljičnog dioksida od (0,81±0,2) g/(kg·dan), indeks klijanja od (105±3) %, maseni udjel ekstraktibilnog amonijaka od 305,78 mg/kg, omjer ugljika i dušika od 16,18, pH=7,6 i električna vodljivost od 3,12 mS/cm potvrđuju zrelost komposta, koji je zadovoljio standarde kompostiranja. U kontrolnom je uzorku opaženo kašnjenje termofilne faze, pa kompost nije sazrio ni nakon 42 dana. Stoga je zaključeno da su dobro pripremljena smjesa za kompostiranje i bakterijska kultura s odgovarajućim svojstvima za razgradnju ulja nužni za uspješan sustav kompostiranja otpada od hrane.The aim of the present study is to develop a good initial composting mix using a bacterial consortium and 2 % lime for effective co-composting of food waste in a 60-litre in-vessel composter. In the experiment that lasted for 42 days, the food waste was first mixed with sawdust and 2 % lime (by dry mass), then one of the reactors was inoculated with an enriched bacterial consortium, while the other served as control. The results show that inoculation of the enriched natural bacterial consortium effectively overcame the oil-laden co-composting mass in the composter and increased the rate of mineralization. In addition, CO2 evolution rate of (0.81±0.2) g/(kg·day), seed germination index of (105±3) %, extractable ammonium mass fraction of 305.78 mg/kg, C/N ratio of 16.18, pH=7.6 and electrical conductivity of 3.12 mS/cm clearly indicate that the compost was well matured and met the composting standard requirements. In contrast, control treatment exhibited a delayed thermophilic phase and did not mature after 42 days, as evidenced by the maturity parameters. Therefore, a good composting mix and potential bacterial inoculum to degrade the oil are essential for food waste co-composting systems

    Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives

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    first_page settings Order Article Reprints Open AccessReview Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives by Sharareh Harirchi 1 [ORCID] , Taner Sar 1 [ORCID] , Mohaddaseh Ramezani 2, Habibu Aliyu 3 [ORCID] , Zahra Etemadifar 4 [ORCID] , Seyed Ali Nojoumi 5,6 [ORCID] , Fatemeh Yazdian 7, Mukesh Kumar Awasthi 8 [ORCID] and Mohammad J. Taherzadeh 1,* [ORCID] 1 Swedish Centre for Resource Recovery, University of Borås, 50190 Borås, Sweden 2 Microorganisms Bank, Iranian Biological Resource Centre (IBRC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran 3 Institute of Process Engineering in Life Science II: Technical Biology, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany 4 Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 8174673441, Iran 5 Microbiology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran 6 Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran 1316943551, Iran 7 Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439957131, Iran 8 College of Natural Resources and Environment, Northwest A&F University, Taicheng Road 3#, Yangling, Xianyang 712100, China * Author to whom correspondence should be addressed. Microorganisms 2022, 10(12), 2355; https://doi.org/10.3390/microorganisms10122355 Received: 26 October 2022 / Revised: 18 November 2022 / Accepted: 21 November 2022 / Published: 28 November 2022 (This article belongs to the Section Microbial Biotechnology) Download Browse Figure Versions Notes Abstract For a long time, the genus Bacillus has been known and considered among the most applicable genera in several fields. Recent taxonomical developments resulted in the identification of more species in Bacillus-related genera, particularly in the order Bacillales (earlier heterotypic synonym: Caryophanales), with potential application for biotechnological and industrial purposes such as biofuels, bioactive agents, biopolymers, and enzymes. Therefore, a thorough understanding of the taxonomy, growth requirements and physiology, genomics, and metabolic pathways in the highly diverse bacterial order, Bacillales, will facilitate a more robust designing and sustainable production of strain lines relevant to a circular economy. This paper is focused principally on less-known genera and their potential in the order Bacillales for promising applications in the industry and addresses the taxonomical complexities of this order. Moreover, it emphasizes the biotechnological usage of some engineered strains of the order Bacillales. The elucidation of novel taxa, their metabolic pathways, and growth conditions would make it possible to drive industrial processes toward an upgraded functionality based on the microbial nature

    Aspergillus spp., a versatile cell factory for enzymes and metabolites: Interventions through genome editing

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    Aspergillus sp. is widely distributed in nature and plays significant roles in the degradation of lignocellulose biomass and extensively used in bioprocess and fermentation technology and many species are also a generally regarded safe. Many of the Aspergillus species are established cell factories due to their inherent capacity in secreting large number of hydrolytic enzymes. With the advent of next generation genomic technologies and metabolic engineering technologies, the production potential of Aspergillus cell factory has improved over the years. Various genome editing tools has been developed for Aspergillus like engineered nucleases, zinc finger nucleases, TALEN and CRISPR-Cas9 system. Currently, the CRISPR/Cas9-based technique is extensively used to enhance the effectiveness of gene manipulation in model system Aspergillus nidulans and other strains like Aspergillus oryzae, Aspergillus niger and Aspergillus fumigatus. This review describes the recent developments of genome editing technologies in Aspergillus the synthesis of heterologous proteins and secondary metabolites in the Aspergillus species

    Enhancing phosphate adsorption by Mg/Al layered double hydroxide functionalized biochar with different Mg/Al ratios

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    Mg/Al ratio plays a significant role for anion adsorption by Mg/Al-layered double hydroxides (Mg/Al-LDHs) modified biochar. In this study, Mg/Al-LDHs biochar with different Mg/Al ratios (2, 3, 4) were prepared by co-precipitation for phosphate removal from aqueous solution. Factors on phosphate adsorption including Mg/Al ratio, pH, and the presence of other inorganic anions were investigated through batch experiments. Increasing Mg/Al ratio in the Mg/Al-LDHs biochar composites generally enhanced phosphate adsorption with Langmuir adsorption maximum calculated at 81.83 mg phosphorous (P) per gram of 4:1 Mg/Al-LDHs biochar at pH 3.0. The adsorption process was best described by the pseudo-second-order kinetic model. Solution pH had greater effects on the phosphate adsorption by Mg/Al LDHs biochar composites with lower Mg/Al ratios. The presence of other inorganic anions decreased the phosphate adsorption efficiency in the order of F- \u3e SO42- \u3e NO2- \u3eCl-. Phosphate adsorption mechanism involves ion exchange, electrostatic attraction and surface inner-sphere complex formation. Overall, Mg/Al-LDHs biochar composites offer a potential alternative of carbon-based adsorbent for phosphate removal from aqueous solution

    High level xylitol production by Pichia fermentans using non-detoxified xylose-rich sugarcane bagasse and olive pits hydrolysates

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    Hemicellulosic sugars, the overlooked fraction of lignocellulosic residues can serve as potential and cost-effective raw material that can be exploited for xylitol production. Xylitol is a top platform chemical with applications in food and pharmaceutical industries. Sugarcane bagasse (SCB) and olive pits (OP) are the major waste streams from sugar and olive oil industries, respectively. The current study evaluated the potential of Pichia fermentans for manufacturing of xylitol from SCB and OP hydrolysates through co-fermentation strategy. The highest xylitol accumulation was noticed with a glucose and xylose ratio of 1:10 followed by feeding with xylose alone. The fed-batch cultivation using pure xylose, SCB, and OP hydrolysates, resulted in xylitol accumulation of 102.5, 86.6 and 71.9 g/L with conversion yield of 0.78, 0.75 and 0.74 g/g, respectively. The non-pathogenic behaviour and ability to accumulate high xylitol levels from agro-industrial residues demonstrates the potential of P. fermentans as microbial cell factory

    Facilitative capture of As(V), Pb(II) and methylene blue from aqueous solutions with MgO hybrid sponge-like carbonaceous composite derived from sugarcane leafy trash

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    Enhancing the contaminant adsorption capacity is a key factor affecting utilization of carbon-based adsorbents in wastewater treatment and encouraging development of biomass thermo-disposal. In this study, a novel MgO hybrid sponge-like carbonaceous composite (HSC) derived from sugarcane leafy trash was prepared through an integrated adsorption-pyrolysis method. The resulted HSC composite was characterized and employed as adsorbent for the removal of negatively charged arsenate (As(V)), positively charged Pb(II), and the organic pollutant methylene blue (MB) from aqueous solutions in batch experiments. The effects of solution pH, contact time, initial concentration, temperature, and ionic strength on As(V), Pb(II) and MB adsorption were investigated. HSC was composed of nano-size MgO flakes and nanotube-like carbon sponge. Hybridization significantly improved As(V), Pb(II) and methylene blue (MB) adsorption when compared with the material without hybridization. The maximum As(V), Pb(II) and MB adsorption capacities obtained from Langmuir model were 157 mg/g, 103 mg/g and 297 mg/g, respectively. As(V) adsorption onto HSC was best fit by the pseudo−second−order model, and Pb(II) and MB with the intraparticle diffusion model. Increased temperature and ionic strength decreased Pb(II) and MB adsorption onto HSC more than As(V). Further FT-IR, XRD and XPS analysis demonstrated that the removal of As(V) by HSC was mainly dominated by surface deposition of MgHAsO4 and Mg(H2AsO4)2 crystals on the HSC composite, while carbon π–π* transition and carbon π–electron played key roles in Pb(II) and MB adsorption. The interaction of Pb(II) with carbon matrix carboxylate was also evident. Overall, MgO hybridization improves the preparation of the nanotube-like carbon sponge composite and provides a potential agricultual residue-based adsorbent for As(V), Pb(II) and MB removal

    Process optimization for recycling of bread waste into bioethanol and biomethane: a circular economy approach

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    Bread is the second most wasted food in the UK with annual wastage of 292,000 tons. In the present work, bread waste (BW) was utilized for fermentative production of ethanol by Saccharomyces cerevisiae KL17. Acidic and enzymatic saccharification of BW was carried out resulting in the highest glucose release of 75 and 97.9 g/L which is 73.5 and 95.9% of theoretical yield, respectively. The obtained sugars were fermented into ethanol initially in shake flask followed by scale up in bioreactor in batch and fed-batch mode. In the fed-batch mode of cultivation, the maximum ethanol titers of 111.3, 106.9, and 114.9 g/L with conversion yield and productivity of 0.48, 0.47, and 0.49 g/g, and 3.1, 3.0, and 3.2 g/L.h was achieved from pure glucose, glucose-rich acidic and enzymatic hydrolysates, respectively. Further to improve the process economics, the solid residues after acidic (ABW) and enzymatic (EBW) hydrolysis of BW along with respective fermentation residues (FR) obtained after the ethanol production were pooled and subjected to anaerobic digestion. The solid residue from ABW + FR, and EBW + FR yielded a biochemical methanation potential (BMP) of 345 and 379 mL CH4/g VS, respectively. Life cycle assessment of the process showed that the total emissions for ethanol production from BW were comparable to the emissions from more established feedstocks such as sugarcane and maize grain and much lower when compared to wheat and sweet potato. The current work demonstrates BW as promising feedstock for sustainable biofuel production with the aid of circular biorefining strategy. To the authors knowledge, this is the first time, such a sequential system has been investigated with BW for ethanol and biomethane production. Further work will be aimed at ethanol production at pilot scale and BMP will be accessed in a commercial anaerobic digester
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