Estimation of genetic parameters for egg production traits in Japanese quail that selected for immune responses and fed different level of dietary L-arginine

This study was conducted in the fields of the Department of Animal Production - College of Agriculture at Kirkuk University for the period from 6/14/2021 to 7/30/2022. 750 quail chicks were supplied from the Poultry Research Station of the Agricultural Research Department in Abu Ghraib. The chicks were fed the starter ration until the age of 30 days, and then they were fed the production ration. At the age of 4 weeks, the chicks were vaccinated with the attenuated Newcastle vaccine, and the chicks were divided into six experimental treatments: the first treatment represented the negative control group that was not vaccinated and did not have the amino acid arginine added to it, the second treatment represented the positive control group whose chicks were vaccinated with the Newcastle vaccine with no arginine added to their diet As for the third and fourth treatments, they were vaccinated with the Newcastle vaccine with the addition of 5% arginine higher than the bird''s needs. The fifth and sixth treatments represented groups of birds vaccinated with the Newcastle vaccine, while feeding the birds with diets to which the amino acid arginine was added, 10% higher than the bird''s needs. A week after the initial immunization, the bird was vaccinated again with Newcastle vaccine, and the immune level was examined by the agglutination test to find out the level of the birds'' immune response, dividing them into two levels of high immune response and low immune response. This process was repeated for the next three generations. The characteristic of egg production at the age of 90 and 120 days, and the egg mass was recorded. The data were analyzed according to the general linear model method using the ready-made statistical program SAS. The genetic equivalent and the genetic and phenotypic correlations were estimated. The characteristics of egg production decreased until the age of 90 and 120 days, as well as the mass of eggs at the age of 120 days, compared to the base population (21.61 vs. 34.84), (22.01 vs. 26.53), (323.47 vs. 545.97), respectively. The egg mass was significantly affected by the level of immunity and the percentage of added arginine, where the highest value of the egg mass was for birds selected for the high immune level and to which arginine was added by 10% higher than the needs of the bird. Estimates of genotyped egg production at 90 and 120 days of age and egg mass at 120 days of age were (0.64, 0.65 and 0.21), respectively. The results showed weak genetic correlations (0.0263) between egg production at the age of 90 days and egg production at the age of 120 days, but the phenotypic correlations were high (0.6248) between egg production at the age of 90 days and egg production at the age of 120 days. The current study indicates that the selection of birds according to the immune response against Newcastle disease led to decrease in egg production and egg mass. Addition the Arginine amino acid did not increase production at the age of 90 and 120 days. While the addition of arginine 10% led to an increase in the egg mass

Effect of adding nigella sativa and saccharomyces cerevisiae on Awassi lambs production performances and blood biochemicals

The study carried out in the college sheep field located in Sayada from Jan 28, 2022 till April 28, 2022 on 16 Awassi lambs distributed into four groups (four lambs per group), with approximately same body weights (20.22 ± 0.56 kg) and age (4 – 4.5 month) respectively. All lambs fed concentrated ration on 2 % base of the live weight with wheat straw as full and free diet during experiment period (90 days), the first group (control) fed normal diet free of dry bread yeast and black seed, the other three groups fed the same concentrate ration with the following supplement: 2 kg bread yeast / ton of feed for 2nd group, 1 kg black seed / ton of feed for 3rd group and 2 kg of bread yeast and kg of black bean / tons of feed for 4th group. At the end of experimental period blood sample obtained from lambs and two lambs from each treatment were slaughtered with average live weights weights 27.41, 25.25, 25.27 and 25.13 kg, The results showed that there was non significant effect of adding bread yeast and black seed in final weight, total and daily weight gain and feed conversion efficiency. Control group outperformed all experimental groups in the feed intake. The results showed that there was a non-significant difference in the PCV, hemoglobin, glucose, cholesterol and total protein levels of blood, while the third treatment was significantly (p≤0.0.5) superior in the percentage of glutathione (GSH) concentration on all treatments. It can be concluded that the addition of bread yeast and black seed did not have any significant effect on the performance of lambs and carcass traits. The study aimed to determine the effect of dry bread yeast and black seed additions on the productive performance of Awassi lambs (growth traits, feed intake, feed conversion efficiency and blood parameters)

Effect of Glibenclamide and Tomato lycopene extract on some biochemical parameters in serum of alloxan Induced diabetic rabbits

Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycaemia resulting from defects in insulin secretion, insulin action, or both, and occurs in almost all populations of the world a variable prevalence. In the present study we evaluated the effect of glibenclamide, and tomato lycopene extract on blood glucose, enzymatic antioxidants, glycated hemoglobin(HbA1c), and malondialdehyde(MDA) in 50 alloxan induced diabetic rabbits. The results showed a significant(P < 0.001) increase in the level of glucose, HbA1c, MDAin alloxan diabetic rabbits in comparison to the control rabbits. A Significant decrease in the level of serum glucose, HbA1c,and MDAin alloxan diabetic rabbits when received glibenclamide daily as single dose 0.5 mg/kg body weight for 20 weeks, and2and 4 mg/kg of tomato lycopene extract for 20 weeks respectively. The result of this study showed a significant (

Heritability and Genetic Correlation Between Productive and Biochemical Traits of White Japanese Quail

&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;Studying heritability and genetic correlation between traits is important to improvement genetic programs and indirect selection, for that, this study was conducted in the poultry field of Animal Production department - College of Agriculture - University of Kirkuk for the period from 28/3/2022 to 15/1/2023, to study heritability and genetic correlation between production and biochemical traits of white Japanese quail. One day hatched white quail chicks were obtained from College of Agriculture - University of Kirkuk, and reared for 35 days until reaching sexual maturity. Then chicks were randomly distributed to 30 families, with a male and a female for each family for two generations. Simple correlation between productive traits and blood biochemical were estimated, and heritability using full sibs method by variance between dam and total variances. The results of the study showed that there was a highly and significant genetic correlation between estrogen hormone in blood serum, egg production and egg mass, while a negative and highly significant genetic correlation was found between estrogen hormone and feed conversion ratio. Heritability values for productive and blood biochemical traits ranged from 0.22 to 0.42 and they considered within mediate heritability. The study recommended to use of selection programs and indirect genetic improvement based on some blood biochemical values (estrogens, total protein and glucose) due to their positive and significant association with productive traits of white Japanese quail

Comparison Between the Effect of Antimlarial Drug Produced by Local

Local isolate of Streptomyces sp.1 showed ability to produce antimalarial activity against P.vivax, it caused a great reduction in the number of the parasite. The inhibition percentages during 24 and 48 hours were 60.90, 88.38 respectively. Purification of antimalarial agent produced by Streptomyces sp.1 was done by extraction with ethylacetate followed by methanol, and application of gel filtration (Sephadex LH-20, Silica gel column) which resulted in brownish compound. This compound named as SAN 1. Drug, antibiotics and SAN1 inhibited the growth of P. vivax in vitro. The highest inhibition value (87.55%) was observed when 1000 g/ml of trimethoprim was added to cultural medium, followed by chloroquine, primaquine, chloramphenicol, tetracycline, streptomycin, SAN1, rifamycin, erythromycin, ampicillin with inhibition percent 86.09, 83.81, 81.74, 78.83, 78.66, 78.42, 75.10, 74.27 and 74.06% respectivel

Longitudinal Twin Growth Discordance Patterns and Adverse Perinatal Outcomes.

OBJECTIVE: The objective of this study was to conduct a longitudinal assessment of inter-twin growth and Doppler discordance, to identify possible distinct patterns, and to investigate the predictive value of longitudinal discordance patterns for adverse perinatal outcomes in twin pregnancies. METHODS: This retrospective cohort study included twin pregnancies followed and delivered at a tertiary University Hospital in London (UK), between 2010 and 2023. We included pregnancies with at least three ultrasound assessments after 18 weeks and delivery after 34 weeks' gestation. Monoamniotic twin pregnancies, pregnancies with twin-to-twin transfusion syndrome, genetic or structural abnormalities, or incomplete data were excluded. Data on chorionicity, biometry, Doppler indices, maternal characteristics, and obstetric as well as neonatal outcomes were extracted from electronic records. Doppler assessment included velocimetry of the umbilical artery, middle cerebral artery and cerebroplacental ratio. Inter-twin growth discordance was calculated for each scan. The primary outcome was a composite of perinatal mortality and neonatal morbidity. Statistical analysis involved multilevel mixed-effects regression models and unsupervised machine learning algorithms, specifically k-means clustering, to identify distinct patterns of inter-twin discordance and their predictive value. Predictive models were compared using the area under the receiver operating characteristics curve, calibration intercept, and slope, validated with repeated cross-validation. Analyses were performed using R, with significance set at p<0.05. RESULTS: Data from a total of 823 twin pregnancies (647 dichorionic, 176 monochorionic) were analyzed. Five distinct patterns of inter-twin growth discordance-low-stable (n=204, 24.8%), mild-decreasing (n=171, 20.8%), low-increasing (n=173, 21.0%), mild-increasing (n=189, 23.0%), and high-stable (n=86, 10.4%)-were derived using an unsupervised learning algorithm that clustered twin pairs based on the progression and patterns of discordance over gestation. In the high-stable cluster, the rates of perinatal morbidity (46.5%, 40/86) and mortality (9.3%, 8/86) were significantly higher, compared to the low-stable (reference) cluster (p<0.001). High-stable growth pattern was also associated with a significantly higher risk of composite adverse perinatal outcomes (Odds ratio 70.19, 95% confidence interval 24.18-299.03, p<0.001; adjusted Odds ratio 76.44, 95% confidence interval 25.39-333.02, p<0.001). The model integrating discordance pattern with CPR discordance at the last ultrasound before delivery demonstrated superior predictive accuracy, evidenced by the highest area under the receiver operating characteristics curve of 0.802 (95% confidence interval 0.712 - 0.892 0.046, p<0.001), compared to only discordance patterns (area under the receiver operating characteristics curve 0.785, 95% confidence interval 0.697 -0.873), intertwin weight discordance at the last ultrasound prior to delivery (area under the receiver operating characteristics curve 0.677, 95% confidence interval 0.545 - 0.809), combination of single measurements of estimated fetal weight and CPR discordance at the last ultrasound prior to delivery (area under the receiver operating characteristics curve 0.702, 95% confidence interval 0.586 -0.818) and single measurement of CPR discordance only at the last ultrasound (area under the receiver operating characteristics curve 0.633, 95% confidence interval 0.515 - 0.751). CONCLUSION: We identified five distinct trajectories of inter-twin fetal growth discordance using an unsupervised machine learning algorithm. Consistent high discordance is associated with increased rates of adverse perinatal outcomes, with a dose-response relationship. Additionally, a predictive model integrating discordance trajectory and CPR discordance at the last visit demonstrated superior predictive accuracy for the prediction of composite adverse perinatal outcomes, compared to either of these measurements alone or a single value of estimated fetal weight discordance at the last ultrasound prior to delivery

Diagnostic challenge for ovarian malignant melanoma in premenopausal women: Primary or metastatic?

<p>Abstract</p> <p>Background</p> <p>In the ovary, metastatic malignant melanoma may be confused with primary malignant melanoma and presents a diagnosis challenge. Most cases are associated with disseminated diseases and poor prognosis. We present this case report of a metastatic ovarian malignant melanoma simulating primary ovarian cancer.</p> <p>Case report</p> <p>A 45-year-old premenopausal woman was incidentally found to have an abdominal mass, 3 years after removal of a cutaneous melanoma lesion. Ultrasound and CT scan revealed left two solid masses, which were found to be an ovarian tumor at laparotomy. Left oophorectomy was performed. Histopathology and immunohistochemistry showed melanoma metastasis to the ovary. Nine months later, the patient developed epilepsy and confusion. Magnetic Resonance Imaging showed unique Wright frontal lobe lesion. She underwent stereotactic radio surgery and dacarbazine monotherapy. For months later, the patient is died from disseminate disease progression.</p> <p>Conclusion</p> <p>Ovarian metastasis is an unusual presentation of cutaneous melanoma and the prognosis was dismal. As illustrated by this case report, a differential diagnosis of a metastatic malignant melanoma must be considered.</p

Effect of surgical experience and spine subspecialty on the reliability of the {AO} Spine Upper Cervical Injury Classification System

OBJECTIVE The objective of this paper was to determine the interobserver reliability and intraobserver reproducibility of the AO Spine Upper Cervical Injury Classification System based on surgeon experience (&lt; 5 years, 5–10 years, 10–20 years, and &gt; 20 years) and surgical subspecialty (orthopedic spine surgery, neurosurgery, and "other" surgery). METHODS A total of 11,601 assessments of upper cervical spine injuries were evaluated based on the AO Spine Upper Cervical Injury Classification System. Reliability and reproducibility scores were obtained twice, with a 3-week time interval. Descriptive statistics were utilized to examine the percentage of accurately classified injuries, and Pearson’s chi-square or Fisher’s exact test was used to screen for potentially relevant differences between study participants. Kappa coefficients (κ) determined the interobserver reliability and intraobserver reproducibility. RESULTS The intraobserver reproducibility was substantial for surgeon experience level (&lt; 5 years: 0.74 vs 5–10 years: 0.69 vs 10–20 years: 0.69 vs &gt; 20 years: 0.70) and surgical subspecialty (orthopedic spine: 0.71 vs neurosurgery: 0.69 vs other: 0.68). Furthermore, the interobserver reliability was substantial for all surgical experience groups on assessment 1 (&lt; 5 years: 0.67 vs 5–10 years: 0.62 vs 10–20 years: 0.61 vs &gt; 20 years: 0.62), and only surgeons with &gt; 20 years of experience did not have substantial reliability on assessment 2 (&lt; 5 years: 0.62 vs 5–10 years: 0.61 vs 10–20 years: 0.61 vs &gt; 20 years: 0.59). Orthopedic spine surgeons and neurosurgeons had substantial intraobserver reproducibility on both assessment 1 (0.64 vs 0.63) and assessment 2 (0.62 vs 0.63), while other surgeons had moderate reliability on assessment 1 (0.43) and fair reliability on assessment 2 (0.36). CONCLUSIONS The international reliability and reproducibility scores for the AO Spine Upper Cervical Injury Classification System demonstrated substantial intraobserver reproducibility and interobserver reliability regardless of surgical experience and spine subspecialty. These results support the global application of this classification system

Elective cancer surgery in COVID-19-free surgical pathways during the SARS-CoV-2 pandemic: An international, multicenter, comparative cohort study

PURPOSE As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19–free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19–free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19–free surgical pathways. Patients who underwent surgery within COVID-19–free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19–free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score–matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19–free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION Within available resources, dedicated COVID-19–free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks

Elective Cancer Surgery in COVID-19-Free Surgical Pathways During the SARS-CoV-2 Pandemic: An International, Multicenter, Comparative Cohort Study.

PURPOSE: As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19-free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS: This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19-free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS: Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19-free surgical pathways. Patients who underwent surgery within COVID-19-free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19-free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score-matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19-free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION: Within available resources, dedicated COVID-19-free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks