Characterization of a natural dye by spectroscopic and chromatographic techniques

11 pages, 7 figures, 2 tables, 29 references.-- Simposio celebrado del 14-19, agosto, 2011, en Cancún, Méjico.Natural dyes have been extracted from both plants and animal to give color to textiles and handicrafts. This is the case of purple dye extracted from Justicia spicigera Schldt, an acanthaceae used as a color source since pre-Hispanic period in the Mayan area of Mexico and Central America. Spectroscopic (UV-Vis and FT-IR) and chromatographic (PY-GC/MS) techniques were employed in order to characterize some of their chemical properties. UV-VIS absorption spectra indicates a λmaxpeak at 581 nm, value associated to anthocyanins group under bathochromic effect. On the other hand, a structural characterization realized by FT-IR and Py-GC/MS indicated the presence of polar hydroxibenzoic acids and phenolic compounds which are characteristics of the molecular structure of anthocyanins.This contribution was possible thanks to the support of the project “Extraction of natural dyes with potential application to restoration of artistic and historic works” from Universidad Autónoma de Campeche.Peer reviewe

“Antimicrobial and antiproliferative activity of essential oil, aqueous and ethanolic extracts of Ocimum micranthum Willd leaves”

Abstract Background Ocimum micranthum Willd is a plant used in traditional medicine practiced in the region of the Yucatan peninsula. In particular, it is used for the treatment of cutaneous infections and wound healing, however there are currently no existing scientific studies that support these applications. The aim of the present study was to evaluate the antimicrobial and the in vitro proliferative activity (on healthy mammalian cell lines) of the essential oil and extracts (aqueous and ethanolic) of this plant. Methods The minimal inhibitory concentration (MIC) of essential oil and aqueous and ethanolic extracts of Ocimum micranthum leaves against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans was determined using the microdilution technique. The in vitro proliferative activity of human fibroblast (hFB) and Chinese hamster ovary (CHO-K1) cells treated with these extracts was evaluated using the MTT test. The hFB cell line was also evaluated using Trypan Blue assay. Results Candida albicans was more susceptible to the ethanolic extract and the aqueous extract (MIC value of 5 μL/mL and 80 μL/mL respectively). In the case of Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa, the MIC of the aqueous and ethanolic extract was 125 μL/mL. The aqueous extract showed a significant (p < 0.05) antiproliferative effect on hFB cells at a concentration of 4%, with cell proliferation percentage values of 73.56% and 20.59% by MTT method and Trypan Blue assay, respectively; the same effect was observed for the ethanolic extract at concentration from 0.06% to 0.25% using MTT method and at a concentration from 0.125% to 0.25% using Trypan Blue assay. In CHO-K1 cells an antiproliferative effect was observed at a concentration of 8% of aqueous extract and from 0.06% to 0.25% of ethanolic extract using the MTT method. Conclusion These assays showed that low concentrations of essential oil and extracts of Ocimum micranthum leaves are sufficient to cause an antiproliferative effect on the hFB cell line but do not produce an antimicrobial effect against the microorganisms evaluated. More studies are necessary to improve understanding of the mechanism of action of the compounds implicated in the bioactivities shown by the crude extracts

Investigación en diseño. Discusiones contemporáneas sobre sus oportunidades y desafíos

Libro que surge como resultado del primer Seminario de los Posgrados en Diseño, realizado en agosto y noviembre de 2023, la obra es un insumo en ese proceso de autorreconocimiento crítico y propositivo de los Posgrados en Diseño, es, de manera general, un diálogo entre los conferencistas y la comunidad, y al mismo tiempo, un posicionamiento epistemológico que afirma la imposibilidad de (re)pensar de manera endógena, improvisada y desconectada, por lo que es imperativo tomar riesgos disciplinares que borren las fronteras del conocimiento y se atrevan al diálogo entre la filosofía, la ciencia, la creatividad, la proyección, así como entre las posturas, miradas y formas, es además de lo anterior, una propuesta ecléctica por convicción. La obra se compone de tres secciones: en la primera se presentan dos capítulos que ofrecen perspectivas sobre la investigación en Diseño desde la arquitectura y la comunicación; la segunda parte la integran otros tres capítulos que discuten el papel del enfoque de Diseño ante grandes problemas locales y nacionales, desde la ciencia política, la economía y la ecología; por último, la tercera parte presenta una serie de relatorías de las seis conferencias presentadas en el Seminario de los Posgrados en Diseño 2023.Francisco Javier de la Torre Galindo, coordinador; Yaszmín Gaviota Montes Altamirano, Imagen de la portada