Effect of Cichorium intybus L. on the expression of hepatic NF-κB and IKKβ and serum TNF-α in STZ− and STZ+ niacinamide-induced diabetes in rats

Additional file 3. Evaluation of metformin. The HPLC system consisted of PLATIN Blue (KNAUER, Germany) with a PDA detector. The wavelength was set at 233 nm. The column was Nucleosil-100, C-18 (250 4.6 mm, 5 nm). The software was EZChrom Elite. The mobile phase comprised 0.01 M potassium dihydrogen orthophosphate (adjusted to pH 4.5 with glacial acetic acid) and acetonitrile (60:40, v/v). Analyses were run at a flow-rate of 1.0 ml/min and the samples were quantified using peak area. Initial weight of each 500 mg pill: Merck, 516.63 mg; and Chemidaru, 582.50 mg. 2.5, 5, 10, 50, and 100 mg of the powdered pills were dissolved in 1 ml of deionized H2O. Merck metformin dissolved completely in water, whereas metformin from Chemidaru consisted of insoluble part. Injection volumes were 5 µl; each sample was injected only once. The corresponding peaks for 10 mg/ml sample and the resulting standard curves for two metformin brands are compared in graphs

Impact of Tramadol and Meloxicam on Oxidative Stress in Dental Extractions due to Periodontal Disease in Dogs

Some diseases, including periodontal diseases as well as anesthesia and drugs, can increase oxidative stress in the body which causes the destruction of cells. Therefore, the effect of tramadol and meloxicam as the two frequently used analgesic drugs on the induction of oxidative stress was investigated in this study. 30 dogs affected with advanced periodontitis which is candidates for dental extraction under general anesthesia were assigned to two different groups randomly. After induction of anesthesia group A received meloxicam (0.2 mg/kg, IV) and group B received tramadol (3 mg/kg, IV) as an analgesic. Prior to injection, at 30 minutes and 60 minutes, and 3 hours after the induction of anesthesia blood samples were collected to measure indicators of oxidative stress including Malondialdehyde (MDA), Superoxide Dismutase (SOD), and Total Antioxidant Capacity (TAC). As a result, an increase in all indicators of oxidative stress was observed at different times measured in both groups. However, the increase was more evident in group B. A significant difference in meanSD increase of MDA and decrease of SOD at 30, 60 min, and 3 hours after the injection was observed between the groups. In conclusion, meloxicam provided fewer imbalances on oxidative stress and therefore, has beneficial effects in dental extractions due to advanced periodontitis in dogs, which is very probable done by regulating the inflammatory and oxidation factors

Effect of Progesterone-only Contraception on Vitamin D in Human Milk

Objective: Nowadays progesterone contraceptives are being used by lactating women. In the last century elements effective in bone growth in infants, such as calcium, phosphorus and vitamin D have been evaluated. Vitamin D has been mentioned mainly in relation to rickets prevention in infants. This study was initiated in order to investigate the effect of Progestin-only contraceptives on breast milk vitamin D. Material & Methods: In this cohort study 138 lactating womenwere assigned to either the hormonal (52 participants) or non-hormonal (86 participants) groups according to their chosen method of contraception. The characteristics and goals of the study were explained in detail and informed consent was obtained from all subjects before inclusion in the study. Research units started their method of contraception 45 (7) days postpartum and continued at least for 6 months afterward. 10 ml milk samples were obtained before the baby nursed at 6 mos postpartum, and kept at -20 °C until analysis. Vitamin D levels were measured with RIA method. Findings: Vitamin D levels were found to be 11.2 (7.2) nmol/L in the hormonal and 10.67±6.6 nmol/L in the non-hormonal groups, which was lower than other cultures. There was not a significant difference between the vitamin D levels of the two groups statistically (P>0.05). Conclusion: According the results, the consumption of progesterone-only contraceptives had no effect on the vitamin D levels of mothers' milk, although with regard to low levels of vitamin D in the milk of lactating mothers, it is necessary to enrich foods with vitamin D and for mothers to use vitamin D supplements

Effect of Progesterone-only Contraception on Vitamin D in Human Milk

Objective: Nowadays progesterone contraceptives are being used by lactating women. In the last century elements effective in bone growth in infants, such as calcium, phosphorus and vitamin D have been evaluated. Vitamin D has been mentioned mainly in relation to rickets prevention in infants. This study was initiated in order to investigate the effect of Progestin-only contraceptives on breast milk vitamin D. Material & Methods: In this cohort study 138 lactating womenwere assigned to either the hormonal (52 participants) or non-hormonal (86 participants) groups according to their chosen method of contraception. The characteristics and goals of the study were explained in detail and informed consent was obtained from all subjects before inclusion in the study. Research units started their method of contraception 45 (7) days postpartum and continued at least for 6 months afterward. 10 ml milk samples were obtained before the baby nursed at 6 mos postpartum, and kept at -20 °C until analysis. Vitamin D levels were measured with RIA method. Findings: Vitamin D levels were found to be 11.2 (7.2) nmol/L in the hormonal and 10.67±6.6 nmol/L in the non-hormonal groups, which was lower than other cultures. There was not a significant difference between the vitamin D levels of the two groups statistically (P>0.05). Conclusion: According the results, the consumption of progesterone-only contraceptives had no effect on the vitamin D levels of mothers' milk, although with regard to low levels of vitamin D in the milk of lactating mothers, it is necessary to enrich foods with vitamin D and for mothers to use vitamin D supplements

Biosynthesis and characterization of gold nanoparticles from citrullus colocynthis (L.) schrad pulp ethanolic extract: Their cytotoxic, genotoxic, apoptotic, and antioxidant activities

The age-old discipline of plant therapy has gained renewed importance through the utilization of plants for the synthesis of metal nanoparticles. However, toxicity testing and characterization of the recently synthesized nanomaterials are essential to evaluating their appropriate application. Citrullus colocynthis is a medicinal plant with several health benefits. Herein, we used its ethanolic pulp extract (PE) to manufacture gold nanoparticles (PE-AuNPs). Various approaches were employed to assess the MTT50 and NR50 values of PE and PE-AuNPs at different concentrations in the human hepatocarcinoma cell line (HepG2). The study aimed to assess the genotoxic effects and in vivo toxicity of PE and PE-AuNPs at MTT50 dosages. The quasi-spherical, cubic/triangular prisms, and nail-looking particles exhibited no antioxidant properties. They had an absorbance peak between 540 and 560 nm, diameters of less than 20 nm, hydrodynamic diameters of 177.9 nm, and a negative surface charge (−10.3 mV). The significant role of plant phytochemicals in the formation of metal nanoparticles is confirmed by the diminished antioxidant capacity of extract residues following PE-AuNP synthesis. PE-AuNPs exhibited in vivo and cytotoxic effects at relatively lower concentrations compared to PE. In contrast to PE, PE-AuNPs exhibited lower genotoxic at MTT50 dosages. Despite having MTT50 values of approximately 1.95 ± 0.06 and 0.89 ± 0.03 mg/ml, PE and PE-AuNPs can still be considered biocompatible. Nonetheless, our results suggest that the characteristics of recently produced nanoparticles can differ from those of the matching plant. Further investigation can provide a better understanding of the possible therapeutic and pharmacological impacts of PE-AuNPs

MOESM2 of Effect of Cichorium intybus L. on the expression of hepatic NF-κB and IKKβ and serum TNF-α in STZ− and STZ+ niacinamide-induced diabetes in rats

Additional file 2. Western blot analysis on 4 members of each group

Differential Expression of Gluconeogenic Enzymes in Early- and Late- Stage Diabetes: The Effect of Citrullus Colocynthis Seed Extract on Hyperglycemia and Hyperlipidemia

Abstract Background: The extent of metabolic disruption and the usefulness of drugs and supplements, such as medicinal plants, in patients with diabetes may be impacted by the severity of the disease. Method: Nicotinamide + STZ together and STZ alone were used to induce early- and late-stage diabetes type 2 (ET2D and LT2D, respectively) in rats. Glucose tolerance test (GTT) was performed because the stage of disease was considered compatible with the magnitude of deviation from normal glucose tolerance test (GTT), as well as the level of FBS. Three main groups of the study were nondiabetic, early-stage diabetic, and late-stage diabetic rats. Each group was divided into two sub-groups, one of which received Citrullus colocynthis seed aqueous extract (CCAE, 90mg/kg BW) for 28 days. Weekly FBS and body weights were recorded during the study. At the end of 28 days, the serum levels of ALT, AST, ALP, TG, urea, creatinine, uric acid, cholesterol, HDL, LDL, c-peptide, and HbA1c were measured; the hepatic mRNA expression of several enzymes of glucose and fat metabolic pathways were also determined by Real-Time PCR. The accumulation of fat in the hepatic tissue was visualized by measuring the TG content and by H&amp;E and Oil-Red staining and the degree of oxidative stress was measured by protein carbonyl content (PCC).Results: The LT2D rats showed maximal deviations from normal GTT. GTT for control and ET2D rats were similar, yet the area under curve (AUC) for ET2D rats was significantly higher. Urea, ALT, and ALP levels were high in diabetic rats compared to control and significantly different from each other.Serum TG dropped and ALT, ALP, HDL and LDL significantly improved after treatment with CCAE. Different patterns of G6Pase and PEPCK expression in ET2D and LT2D suggested their similarity to short- and long-term fasting states, respectively. While the reduction of FBS levels in ET2D could be explained by an inhibition of G6Pase expression and therefore glycogenolysis, the level of PEPCK expression was not significantly lowered by CCAE in LT2D. H&amp;E staining of liver tissue showed steatoses of varying morphology in both ET2D and LT2D rats. CCAE led to up-regulated PPARα and down-regulated CPT1 expressions.Conclusion: As PEPCK activity is necessary for the continuation of the TG/FA cycle during fasting, it is possible that in LT2D, CCAE directed the PEPCK activity more towards glyceroneogenesis than gluconeogenesis to ensure the persistence of the TG/FA cycle. The enhanced glyceroneogenesis together with an up-regulated PPARa expression and down-regulated CPT1 expression probably led to lower blood and hepatic TG. More research is needed to establish the effect of CCAE on PEPCK expression and its course of activity.</jats:p