Novel Lubricant Compressor For Automotive Air Conditioning System

Outside Temperature increase – Global warming, El-Nino, etc. Cooling capacity reduce – Compressor work increase Automotive Air Conditioning (AAC) – increase fuel Consumption up to 20% & Greenhouse gas NOx (80%) & CO (70%) Current AAC system is been optimized but still could not cope with today’s weather condition

Persepsi Pemustaka Tentang Sikap Pustakawan pada Layanan Sirkulasi di Perpustakaan Daerah Jepara

Penelitian ini dilakukan untuk mengetahui bagaimana sikap pustakawan pada layanan sirkulasi di Perpustakaan Daerah Jepara. Desain penelitian yang digunakan adalah penelitian Kualitatif dengan menggunakan analisis deskriptif. Metode pengumpulan data yang digunakan meliputi wawancara kepada informan untuk melihat secara langsung persepsi pemustaka tentang sikap pustakawan pada layanan sirkulasi di perpustakaan, dan studi pustaka untuk mengumpulkan informasi yang berkaitan dengan penelitian ini. Analisis yang digunakan yaitu analisis deskriptif. Dari hasil analisis diketahui bahwa persepsi pemustaka tentang sikap pustakawan di layanan sirkulasi Perpustakaan Daerah Jepara dari aspek kehandalan, terkait dengan daya tanggap pustakawan, akurasi atau kesesuaian pelayanan pustakawan, kompetensi, dan komunikatif ini cukup baik; tetapi dalam daya tanggap merespon untuk melakukan sesuatu dirasa kurang. Aspek empati (sikap simpatik dan kesopanan pustakawan) baik, sementara sebagian pustakawan kurang simpatik. Aspek perhatian (sikap kepedulian, sikap tindak lanjut dan proaktif) baik dalam sikap tindak lanjut, cukup baik dalam kepedulian, sementara beberapa pustakawan yang kurang peduli dan proakti

Synthesis of ethyl acetate employing celite-immobilized lipase of Bacillus cereus MTCC 8372

A wide range of fatty acid esters can be synthesized by esterification and transesterification reactions catalyzed by lipases in non-aqueous systems. In the present study, immobilization of a purified alkaline extra-cellular lipase of Bacillus cereus MTCC 8372 by adsorption on diatomaceous earth (celite) for synthesis of ethyl acetate via transesterification route was investigated. B. cereus lipase was deposited on celite (77% protein binding efficiency) by direct binding from aqueous solution. Immobilized lipase was used to synthesis of ethyl acetate from vinyl acetate and ethanol in n -nonane. Various reaction conditions, such as biocatalyst concentration, substrates concentration, choices of solvents ( n -alkanes), incubation time, temperature, molecular sieves (3&Aring; &times; 1.5 mm), and water activity(a w ), were optimized. The immobilized lipase (25 mg/ml) was used to perform transesterification in n -alkane(s) that resulted in approximately 73.7 mM of ethyl acetate at 55 &deg;C in n -nonane under shaking (160 rpm) after 15 h, when vinyl acetate and ethanol were used in a equimolar ratio (100 mM each). Addition of molecular sieves (3&Aring; &times; 1.5 mm) as well as effect of water activity of saturated salt solutions (KI, KCl and KNO 3 ) to the transesterification efficiency has inhibitory effect. Batch operational stability tests indicated that immobilized lipase had retained 50% of its original catalytic activity after four consecutive batches of 15 h each.<br /

Microbial lipases : at the interface of aqueous and non-aqueous media: a review

In recent times, biotechnological applications of microbial lipases in synthesis of many organic molecules have rapidly increased in non-aqueous media. Microbial lipases are the working horses\u27 in biocatalysis and have been extensively studied when their exceptionally high stability in non-aqueous media has been discovered. Stability of lipases in organic solvents makes them commercially feasibile in the enzymatic esterification reactions. Their stability is affected by temperature, reaction medium, water concentration and by the biocatalyst\u27s preparation. An optimization process for ester synthesis from pilot scale to industrial scale in the reaction medium is discussed. The water released during the esterification process can be controlled over a wide range and has a profound effect on the activity of the lipases. Approaches to lipase catalysis like protein engineering, directed evolution and metagenome approach were studied. This review reports the recent development in the field of non-aqueous microbial lipase catalysis and factors controlling the esterification/transesterification processes in organic media

Multisystem proteinopathy due to a homozygous p.Arg159His VCP mutation : a tale of the unexpected

ObjectiveTo assess the clinical, radiologic, myopathologic, and proteomic findings in a patient manifesting a multisystem proteinopathy due to a homozygous valosin-containing protein gene (VCP) mutation previously reported to be pathogenic in the heterozygous state.MethodsWe studied a 36-year-old male index patient and his father, both presenting with progressive limb-girdle weakness. Muscle involvement was assessed by MRI and muscle biopsies. We performed whole-exome sequencing and Sanger sequencing for segregation analysis of the identified p.Arg159His VCP mutation. To dissect biological disease signatures, we applied state-of-the-art quantitative proteomics on muscle tissue of the index case, his father, 3 additional patients with VCP-related myopathy, and 3 control individuals.ResultsThe index patient, homozygous for the known p.Arg159His mutation in VCP, manifested a typical VCP-related myopathy phenotype, although with a markedly high creatine kinase value and a relatively early disease onset, and Paget disease of bone. The father exhibited a myopathy phenotype and discrete parkinsonism, and multiple deceased family members on the maternal side of the pedigree displayed a dementia, parkinsonism, or myopathy phenotype. Bioinformatic analysis of quantitative proteomic data revealed the degenerative nature of the disease, with evidence suggesting selective failure of muscle regeneration and stress granule dyshomeostasis.ConclusionWe report a patient showing a multisystem proteinopathy due to a homozygous VCP mutation. The patient manifests a severe phenotype, yet fundamental disease characteristics are preserved. Proteomic findings provide further insights into VCP-related pathomechanisms

Comparison of Convective Heat Transfer Coefficient and Friction Factor of TiO2 Nanofluid flow in a tube with Twisted Tape Inserts

Nanofluids have gained extensive attention due to their role in improving the efficiency of thermal systems. The present study reports a further enhancement in heat transfer coefficients in combination with structural modifications of flow systems namely, the addition of tape inserts. Experiments are undertaken to determine heat transfer coefficients and friction factor of TiO2/water nanofluid up to 3.0% volume concentration at an average temperature of 30 C. The investigations are undertaken in the Reynolds number range of 8000-30,000 for flow in tubes and with tapes of different twist ratios. A significant enhancement of 23.2% in the heat transfer coefficients is observed at 1.0% concentration for flow in a tube. With the use of twisted tapes, the heat transfer coefficient increased with decrease in twist ratio for water and nanofluid. The heat transfer coefficient and friction factor are respectively 81.1% and 1.5 times greater at Re ¼ 23,558 with 1.0% concentration and twist ratio of 5, compared to values with flow of water in a tube. An increase in the nanofluid concentration to 3.0% decreased heat transfer coefficients to values lower than water for flow in a tube and with tape inserts. A thermal system with tape insert of twist ratio 15 and 1.0% TiO2 concentration gives maximum advantage ratio, if pressure drop is considered along with enhancement in heat transfer coefficient

DNA fingerprinting of red jungle fowl, village chicken and broilers

The genomic mapping of Red Jungle Fowl (Gallus gallus), local Village Chicken, and broiler was carried out by random amplified polymorphism DNA (RAPD) technique. Two different sets of arbitrary primers were used (Operon OPAO1-20 and Genemed GM01-50). All the genomes of the three species of chickens were amplified with OPA01-20 primers. The genomes of the Red Jungle Fowl and local Village Chicken were further amplified with GM01-50 primers. Analysis of the results based on band sharing (BS) and the molecular size of individually amplified DNA fragments showed that Red Jungle Fowl and local Village Chicken shared the species similarity of 66% with Operon primers 01-20, 64% between local Village Chicken and broiler, and 63% when DNA bands between Red Jungle Fowl and broiler were compared. With GM01-50, the BS between Red Jungle Fowl and local village chicken increased to 72%. The results showed that the local village chicken is more closely related to Red Jungle Fowl than to broiler in the genetic distance. On the other hand, broiler is 1% closer in genetic distance to local village chicken than to Red Jungle Fowl. The results also indicated that primers like OPA-7, 8 and 9 can be used as species specific DNA markers for these three species of chickens

Thermal Conductivity and Viscosity Of Al2o3 Nanofluids for Different Based Ratio of Water and Ethylene Glycol Mixture

In the thermal engineering applications, suspension of nanoparticles in conventional fluid has positive potential in enhancing the convective heat transfer performance. The evaluation of thermo-physical properties is essential to investigate the forced convection heat transfer of nanofluids. Hence, the present study reports the analysis on thermal conductivity and dynamic viscosity for Al2O3 nanoparticle dispersed in a different volume ratio of water (W) and ethylene glycol (EG) mixture. The Al2O3 nanofluids are formulated using the two-step method for three different base mixtures with volume ratio of 40:60, 50:50 and 60:40 (W:EG). The measurement of thermal conductivity and viscosity were performed using KD2 Pro Thermal Properties Analyzer and Brookfield LVDV-III Rheometer; respectively for temperature from 30 to 70 °C and volume concentration of 0.2–1.0%. The average thermal conductivity enhancement of Al2O3 nanofluids in the three base ratios varied from 2.6 to 12.8%. The nanofluids have better enhancement as the percentage of ethylene glycol increases. Meanwhile, the average dynamic viscosity enhanced up to 50% for 60:40 (W:EG). The enhancement of viscosity for nanofluids decreased with the increment percentage of ethylene glycol. The properties enhancement of the Al2O3 nanofluids is significantly influenced by the concentration, temperature, and based ratio

Corneal Confocal Microscopy Detects Small Fibre Neuropathy in Patients with Upper Gastrointestinal Cancer and Nerve Regeneration in Chemotherapy Induced Peripheral Neuropathy.

Published onlineThere are multiple neurological complications of cancer and its treatment. This study assessed the utility of the novel non-invasive ophthalmic technique of corneal confocal microscopy in identifying neuropathy in patients with upper gastrointestinal cancer before and after platinum based chemotherapy. In this study, 21 subjects with upper gastrointestinal (oesophageal or gastric) cancer and 21 healthy control subjects underwent assessment of neuropathy using the neuropathy disability score, quantitative sensory testing for vibration perception threshold, warm and cold sensation thresholds, cold and heat induced pain thresholds, nerve conduction studies and corneal confocal microscopy. Patients with gastro-oesophageal cancer had higher heat induced pain (P = 0.04) and warm sensation (P = 0.03) thresholds with a significantly reduced sural sensory (P<0.01) and peroneal motor (P<0.01) nerve conduction velocity, corneal nerve fibre density (CNFD), nerve branch density (CNBD) and nerve fibre length (CNFL) (P<0.0001). Furthermore, CNFD correlated significantly with the time from presentation with symptoms to commencing chemotherapy (r = -0.54, P = 0.02), and CNFL (r = -0.8, P<0.0001) and CNBD (r = 0.63, P = 0.003) were related to the severity of lymph node involvement. After the 3rd cycle of chemotherapy, there was no change in any measure of neuropathy, except for a significant increase in CNFL (P = 0.003). Corneal confocal microscopy detects a small fibre neuropathy in this cohort of patients with upper gastrointestinal cancer, which was related to disease severity. Furthermore, the increase in CNFL after the chemotherapy may indicate nerve regeneration