Reproductive inequalities in the acanthocephalan Corynosoma cetaceum: looking beyond "crowding" effects

Background: At present, much research effort has been devoted to investigate overall (average) responses of parasite populations to specific factors, e.g., density-dependence in fecundity or mortality. However, studies on parasite populations usually pay little attention to individual variation (inequality) in reproductive success. A previous study on the acanthocephalan Corynosoma cetaceum in franciscana dolphins, Pontoporia blainvillei, revealed no overall intensity-dependent, or microhabitat effects, on mass and fecundity of worms. In this study, we investigated whether the same factors could influence mass inequalities for this species of acanthocephalan.Methods: A total of 10,138 specimens of C. cetaceum were collected from 10 franciscana dolphins accidentally caught in Buenos Aires Province between 1988-1990. To investigate mass inequalities, all the specimens were sexed, and females were classified according to their developmental stage and weighted. Additionally, the relationship between biomass and fecundity (estimated as the number of acanthors) was investigated for some females. Inequalities in fecundity and biomass were assessed using standard methods, i.e. the Lorenz curve and the Gini coefficient (G).Results: We found a modest, but highly significant linear relationship between mass and fecundity. The G was very low (0.314) compared with that from other helminth species. G values were significantly lower in gravid females, which presumably exhibit a slow rate of growth. Also, G values significantly increased with total intensity, but only for gravid females, and the effect was more predictable considering only the number of gravid females.Conclusions: Apparently, competition between reproducing females increases inequality without producing crowding effects. Although the mechanism whereby this occurs is unclear, gravid females, at higher intensities, expanded their distribution and occupied gut chambers with contrasting environmental conditions, which might result in greater variability in body size. The observed inequalities are not expected to strongly influence the population genetics of C. cetaceum, but they reveal subtle individual effects beyond an overall population impact.Fil: Aznar Avendaño, Francisco Javier. Universidad de Valencia; EspañaFil: Hernández Orts, Jesús Servando. Universidad Nacional del Comahue. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". - Provincia de Río Negro. Ministerio de Agricultura, Ganadería y Pesca. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Centro Nacional Patagónico. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni"; ArgentinaFil: Vélez Rubio, Gabriela Manuela. Universidad de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de la República; UruguayFil: Fernández, Luis M.. Ceiba Foundation for Tropical Conservation; Estados UnidosFil: Muriel, Nadia T.. No especifíca;Fil: Raga, Juan Antonio. Universidad de Valencia; Españ

Complicaciones tras el enclavado de Ender en las fracturas trocantéreas

—Se realizó el análisis de las complicaciones de 100 casos consecutivos de pacientes con fractura trocantérea del fémur intervenidos mediante clavos de Ender, con un seguimiento postoperatorio medio de 10 meses. Hubo 75 mujeres y 25 hombres, con una media de edad de 81 años. Según la clasificación de Jensen un 27% eran estables y un 73% inestables. Se obtuvieron un 32% de complicaciones y errores de técnica intraoperatorios, un 39% de migraciones distales, un 3% de migraciones proximales y un 11% de consolidaciones viciosas en varo. Todo ello se tradujo en un 21% de reintervenciones.The rate and type of complications following Ender nailing in a series of 100 consecutive patients with trochanteric hip fractures were analysed. There were 75 women and 25 men with a mean age of 81 years. According to Jensen classification, 27% were stable fractures and 73% unstable. There were registered 32% of intraoperative complications and technical errors, 39% of distal migration of nails, 3% of proximal migrations with perforations of the femoral head and 11% of healing malpositions in varus. The rale of reoperations was 21%

TREX-DM: a low background Micromegas-based TPC for low-mass WIMP detection

Dark Matter experiments are recently focusing their detection techniques in low-mass WIMPs, which requires the use of light elements and low energy threshold. In this context, we describe the TREX-DM experiment, a low background Micromegas-based TPC for low-mass WIMP detection. Its main goal is the operation of an active detection mass $\sim$0.3 kg, with an energy threshold below 0.4 keVee and fully built with previously selected radiopure materials. This work describes the commissioning of the actual setup situated in a laboratory on surface and the updates needed for a possible physics run at the Canfranc Underground Laboratory (LSC) in 2016. A preliminary background model of TREX-DM is also presented, based on a Geant4 simulation, the simulation of the detector's response and two discrimination methods: a conservative muon/electron and one based on a neutron source. Based on this background model, TREX-DM could be competitive in the search for low-mass WIMPs. In particular it could be sensitive, e.g., to the low-mass WIMP interpretation of the DAMA/LIBRA and other hints in a conservative scenario.Comment: Proceedings of the XIV International Conference on Topics in Astroparticle and Underground Physics (TAUP 2015), 7-11 September 2015, Torino, Ital

New Insights Into Sunflower (Helianthus annuus L.) FatA and FatB Thioesterases, Their Regulation, Structure and Distribution

Sunflower seeds (Helianthus annuus L.) accumulate large quantities of triacylglycerols (TAG) between 12 and 28 days after flowering (DAF). This is the period of maximal acyl-acyl carrier protein (acyl-ACP) thioesterase activity in vitro, the enzymes that terminate the process of de novo fatty acid synthesis by catalyzing the hydrolysis of the acyl-ACPs synthesized by fatty acid synthase. Fatty acid thioesterases can be classified into two families with distinct substrate specificities, namely FatA and FatB. Here, some new aspects of these enzymes have been studied, assessing how both enzymes contribute to the acyl composition of sunflower oil, not least through the changes in their expression during the process of seed filling. Moreover, the binding pockets of these enzymes were modeled based on new data from plant thioesterases, revealing important differences in their volume and geometry. Finally, the subcellular location of the two enzymes was evaluated and while both possess an N-terminal plastid transit peptide, only in FatB contains a hydrophobic sequence that could potentially serve as a transmembrane domain. Indeed, using in vivo imaging and organelle fractionation, H. annuus thioesterases, HaFatA and HaFatB, appear to be differentially localized in the plastid stroma and membrane envelope, respectively. The divergent roles fulfilled by HaFatA and HaFatB in oil biosynthesis are discussed in the light of our data.España MINECO y FEDER Projects AGL2014- 53537-R y AGL2017-83449-

Técnica de Ender y tornillo-placa deslizante a compresión en el tratamiento de las fracturas trocantéreas

Se realizó un estudio comparativo de 195 fracturas troncantéreas del fémur tratadas quirúrgicamente de las que 112 lo fueron mediante enclavado endomedular de Ender y 83 mediante tornillo-placa deslizante a compresión (TPDC). No encontramos diferencias significativas de las pérdidas sanguíneas entre las dos técnicas. El TPDC presentó mejores resultados funcionales salvo en pacientes mayores de 80 años en que son similares ambas técnicas. El enclavado Ender presentó mayor número de complicaciones mecánicas y reintervenciones.A comparative study on 195 throchanttric hip fractures treated Esther by Ender's mailing (112 cases) and slidding screw-plate (83 cases) were performed. There were no statistically significant differences between the two techniques regarding blood loosening. The slidding screw-plate showed better functional results, except in cases over 80 years old where both techniques were found to be similar. Ender's nailing disclosed a higher number of mechanical and reoperations

A Micromegas-based low-background x-ray detector coupled to a slumped-glass telescope for axion research

We report on the design, construction and operation of a low background x-ray detection line composed of a shielded Micromegas (micromesh gaseous structure) detector of the microbulk technique. The detector is made from radiopure materials and is placed at the focal point of a $\sim$~5 cm diameter, 1.3 m focal-length, cone-approximation Wolter I x-ray telescope (XRT) comprised of thermally-formed (or "slumped") glass substrates deposited with multilayer coatings. The system has been conceived as a technological pathfinder for the future International Axion Observatory (IAXO), as it combines two of the techniques (optic and detector) proposed in the conceptual design of the project. It is innovative for two reasons: it is the first time an x-ray optic has been designed and fabricated specifically for axion research, and the first time a Micromegas detector has been operated with an x-ray optic. The line has been installed at one end of the CERN Axion Solar Telescope (CAST) magnet and is currently looking for solar axions. The combination of the XRT and Micromegas detector provides the best signal-to-noise ratio obtained so far by any detection system of the CAST experiment with a background rate of 5.4$\times$10$^{-3}\;$counts per hour in the energy region-of-interest and signal spot area.Comment: 21 pages, 16 figure

Zeeman tomography of magnetic white dwarfs. II, The quadrupole-dominated magnetic field of HE 1045-0908

We report time-resolved optical flux and circular polarization spectroscopy of the magnetic DA white dwarf HE 1045−0908 obtained with FORS1 at the ESO VLT. Considering published results, we estimate a likely rotational period of P rot 2.7 h, but cannot exclude values as high as about 9 h. Our detailed Zeeman tomographic analysis reveals a field structure which is dominated by a quadrupole and contains additional dipole and octupole contributions, and which does not depend strongly on the assumed value of the period. A good fit to the Zeeman flux and polarization spectra is obtained if all field components are centred and inclinations of their magnetic axes with respect to each other are allowed for. The fit can be slightly improved if an offset from the centre of the star is included. The prevailing surface field strength is 16 MG, but values between 10 and ∼ 75 MG do occur. We derive an effective photospheric temperature of HE 1045−0908 of T eff = 10 000 ± 1000 K. The tomographic code makes use of an extensive database of pre-computed Zeeman spectra (Paper I)

X-ray detection with Micromegas with background levels below 10−6^{-6} keV−1^{-1}cm−2^{-2}s−1^{-1}

Micromegas detectors are an optimum technological choice for the detection of low energy x-rays. The low background techniques applied to these detectors yielded remarkable background reductions over the years, being the CAST experiment beneficiary of these developments. In this document we report on the latest upgrades towards further background reductions and better understanding of the detectors' response. The upgrades encompass the readout electronics, a new detector design and the implementation of a more efficient cosmic muon veto system. Background levels below 10$^{-6}$keV$^{-1}$cm$^{-2}$s$^{-1}$ have been obtained at sea level for the first time, demonstrating the feasibility of the expectations posed by IAXO, the next generation axion helioscope. Some results obtained with a set of measurements conducted in the x-ray beam of the CAST Detector Laboratory will be also presented and discussed