Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

© 2015 Webster et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The attached file is the published version of the article

Influence of fluoride on the mineralization of collagen via the polymer-induced liquid-precursor (PILP) process.

ObjectiveThe polymer-induced liquid-precursor (PILP) mineralization process has been shown to remineralize artificial dentin lesions to levels consistent with those of native dentin. However, nanoindentation revealed that the moduli of those remineralized lesions were only ∼50% that of native dentin. We hypothesize that this may be due to the PILP process having been previously optimized to obtain high amounts (∼70wt%) of intrafibrillar crystals, but without sufficient interfibrillar mineral, another significant component of dentin.MethodsFluoride was added to the PILP-mineralization of collagen from rat tail tendon at varying concentrations to determine if a better balance of intra- versus inter-fibrillar mineralization could be obtained, as determined by electron microscopy. Nanoindentation was used to determine if fluoridated apatite could improve the mechanical properties of the composites.ResultsFluoride was successfully incorporated into the PILP-mineralization of rat tail tendon and resulted in collagen-mineral composite systems with the mineral phase of hydroxyapatite containing various levels of fluoridation. As the fluoride concentration increased, the crystals became larger and more rod-like, with an increasing tendency to form on the fibril surfaces rather than the interior. Nanomechanical testing of the mineralized tendons revealed that fluoride addition did not increase modulus over PILP mineralization alone. This likely resulted from the separated nature of collagen fibrils that comprise tendon, which does not provide lateral reinforcement and therefore may not be suited for the compressive loads of nanoindentation.SignificanceThis work contributes to the development of minimally invasive approaches to caries treatment by determining if collagen can be functionally mineralized

Randomized Quasi-Newton Updates are Linearly Convergent Matrix Inversion Algorithms

We develop and analyze a broad family of stochastic/randomized algorithms for inverting a matrix. We also develop specialized variants maintaining symmetry or positive definiteness of the iterates. All methods in the family converge globally and linearly (i.e., the error decays exponentially), with explicit rates. In special cases, we obtain stochastic block variants of several quasi-Newton updates, including bad Broyden (BB), good Broyden (GB), Powell-symmetric-Broyden (PSB), Davidon-Fletcher-Powell (DFP) and Broyden-Fletcher-Goldfarb-Shanno (BFGS). Ours are the first stochastic versions of these updates shown to converge to an inverse of a fixed matrix. Through a dual viewpoint we uncover a fundamental link between quasi-Newton updates and approximate inverse preconditioning. Further, we develop an adaptive variant of randomized block BFGS, where we modify the distribution underlying the stochasticity of the method throughout the iterative process to achieve faster convergence. By inverting several matrices from varied applications, we demonstrate that AdaRBFGS is highly competitive when compared to the well established Newton-Schulz and minimal residual methods. In particular, on large-scale problems our method outperforms the standard methods by orders of magnitude. Development of efficient methods for estimating the inverse of very large matrices is a much needed tool for preconditioning and variable metric optimization methods in the advent of the big data era

The contribution of the Unresolved Extragalactic Radio Sources to the Brightness Temperature of the sky

The contribution of the Unresolved Extragalactic Radio Sources to the diffuse brightness of the sky was evaluated using the source number - flux measurements available in literature. We first optimized the fitting function of the data based on number counts distribution. We then computed the brightness temperature at various frequencies from 151 MHz to 8440 MHz and derived its spectral dependence. As expected the frequency dependence can be described by a power law with a spectral index $\gamma \simeq -2.7$, in agreement with the flux emitted by the {\it steep spectrum} sources. The contribution of {\it flat spectrum} sources becomes relevant at frequencies above several GHz. Using the data available in literature we improved our knowledge of the brightness of the unresolved extragalactic radio sources. The results obtained have general validity and they can be used to disentangle the various contributions of the sky brightness and to evaluate the CMB temperature.Comment: Accepted for publication in the Astrophysical Journa

A Methodology and Tool for Rapid Prototyping of Data Warehouses using Data Mining: Application to Birds Biodiversity

International audienceData Warehouses (DWs) are large repositories of data aimed at supporting the decision-making process by enabling flexible and interactive analyses via OLAP systems. Rapid prototyping of DWs is necessary when OLAP applications are complex. Some work about the integration of Data Mining and OLAP systems has been done to enhance OLAP operators with mined indicators, and/or to define the DW schema. However, to best of our knowledge, prototyping methods for DWs do not support this kind of integration. Then, in this paper we present a new prototyping methodology for DWs, extending [3], where DM methods are used to define the DW schema. We validate our approach on a real data set concerning bird biodiversity

RC J0311+0507: A Candidate for Superpowerful Radio Galaxies in the Early Universe at Redshift z=4.514

A strong emission line at 6703A has been detected in the optical spectrum for the host galaxy (R=23.1) of the radio source RC J0311+0507 (4C+04.11). This radio galaxy, with a spectral index of 1.31 in the frequency range 365-4850 MHz, is one of the ultrasteep spectrum objects from the deep survey of a sky strip conducted with RATAN-600 in 1980-1981. We present arguments in favor of the identification of this line with Ly\alpha at redshift z=4.514. In this case, the object belongs to the group of extremely distant radio galaxies of ultrahigh radio luminosity (P_{1400}=1.3 x 10^{29}W Hz^{-1}). Such power can be provided only by a fairly massive black hole (~10^9M_\sun}) that formed in a time less than the age of the Universe at the observed z(1.3 Gyr) or had a primordial origin.Comment: 8 pages, 3 figure

Radiative Efficiency and Content of Extragalactic Radio Sources: Toward a Universal Scaling Relation Between Jet Power and Radio Power

We present an analysis of the energetics and particle content of the lobes of 24 radio galaxies at the cores of cooling clusters. The radio lobes in these systems have created visible cavities in the surrounding hot, X-ray-emitting gas, which allow direct measurement of the mechanical jet power of radio sources over six decades of radio luminosity, independently of the radio properties themselves. Using these measurements, we examine the ratio between radio power and total jet power (the radiative efficiency). We find that jet (cavity) power increases with radio synchrotron power approximately as P_jet ~ (L_radio)^beta, where 0.35 < beta < 0.70 depending on the bandpass of measurement and state of the source. However, the scatter about these relations caused by variations in radiative efficiency spans more than four orders of magnitude. After accounting for variations in synchrotron break frequency (age), the scatter is reduced by ~ 50%, yielding the most accurate scaling relation available between the lobe bolometric radio power and the jet (cavity) power. We place limits on the magnetic field strengths and particle content of the radio lobes using a variety of X-ray constraints. We find that the lobe magnetic field strengths vary between a few to several tens of microgauss depending on the age and dynamical state of the lobes. If the cavities are maintained in pressure balance with their surroundings and are supported by internal fields and particles in equipartition, the ratio of energy in electrons to heavy particles (k) must vary widely from approximately unity to 4000, consistent with heavy (hadronic) jets.Comment: 20 pages, 11 figures. Accepted for publication in Ap

Primary physical education, coaches and continuing professional development

This is an Author's Accepted Manuscript of an article published in Sport, Education and Society, 16(4), 485 - 505, 2011, copyright @ Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/13573322.2011.589645.Physical education (PE) in primary schools has traditionally been taught by qualified primary teachers. More recently, some teaching of PE in primary schools has been undertaken by coaches (mostly football coaches). These coaches hold national governing body awards but do not hold teaching qualifications. Thus, coaches may not be adequately prepared to teach PE in curriculum time. The purpose of this study was to evaluate the perceptions of a group of community-based football coaches working in primary schools for the impact of a Continuing Professional Development (CPD) programme on their ability to undertake ‘specified work’ to cover PE in primary schools. The programme focused on four areas identified as important to enable coaches to cover specified work: short- and medium-term planning, pedagogy, knowledge of the curriculum and reflection. Results showed that for the majority of coaches the CPD programme had made them more aware of the importance of these four areas and had helped to develop their knowledge and ability to put this into practice in covering planning, preparation and assessment time. However, further input is still required to develop coaches’ knowledge and understanding in all four areas, but especially their curriculum knowledge, as well as their ability to put these into practice consistently. These findings are discussed in relation to the implications of employing coaches to cover the teaching of PE in primary schools and, if employed, what CPD coaches need to develop the necessary knowledge, skill and understanding for covering specified work in schools