Differential expression of the brassinosteroid receptor-encoding BRI1 gene in Arabidopsis

Abstract Brassinosteroid (BR)-regulated growth and development in Arabidopsis depends on BRASSINOSTEROID INSENSITIVE 1 (BRI1), the BR receptor that is responsible for initiating the events of BR signalling. We analysed the temporal and spatial regulation of BRI1 expression using stable transgenic lines that carried BRI1 promoter:reporter fusions. In both seedlings and mature plants the tissues undergoing elongation or differentiation showed elevated BRI1 gene activity, and it could be demonstrated that in the hypocotyl this was accompanied by accumulation of the BRI1 transcript and its receptor protein product. In seedlings the BRI1 promoter was also found to be under diurnal regulation, determined primarily by light repression and a superimposed circadian control. To determine the functional importance of transcriptional regulation we complemented the severely BR insensitive bri1-101 mutant with a BRI1-luciferase fusion construct that was driven by promoters with contrasting specificities. Whereas the BRI1 promoter-driven transgene fully restored the wild phenotype, expression from the photosynthesisassociated CAB3 and the vasculature-specific SUC2 and ATHB8 promoters resulted in plants with varying morphogenic defects. Our results reveal complex differential regulation of BRI1 expression, and suggest that by influencing the distribution and abundance of the receptor this regulation can enhance or attenuate BR signalling

Effect of ultrasound on banana cv Pacovan drying kinetics.

The aim of this work was to study and to model the drying kinetics of fresh and ultrasonic pretreated banana cv Pacovan using the diffusional model (Fick?s second law) and an empirical two parameters model (Page model). The pretreatment was carried out in an ultrasonic bath at 30 °C. The drying process was carried out in a fixed bed dryer at two different temperatures (50 and 70 °C) and 3.0 m/s air velocity. Page empirical model provided the best simulation of the drying curves. The diffusional model was used to describe the moisture transfer and the effective diffusivities of water were determined and were in the order of 10 9 m 2 /s. These diffusivities increased with increasing temperature and with the application of ultrasound, while the process time reduced, which can represent an economy of energy, since air drying is cost intensive