Measuring the atomic recoil frequency using a perturbative grating-echo atom interferometer

We describe progress toward a precise measurement of the recoil energy of an atom measured using a perturbative grating-echo atom interferometer (AI) that involves three standing-wave (sw) pulses. With this technique, a perturbing sw pulse is used to shift the phase of excited momentum states---producing a modulation in the contrast of the interference pattern. The signal exhibits narrow fringes that revive periodically at twice the two-photon recoil frequency, $2\omega_q$, as a function of the onset time of the pulse. Experiments are performed using samples of laser-cooled rubidium atoms with temperatures $\lesssim 5$ $\mu$K in a non-magnetic apparatus. We demonstrate a measurement of $\omega_q$ with a statistical uncertainty of 37 parts per $10^9$ (ppb) on a time scale of $\sim 45$ ms in 14 hours. Further statistical improvements are anticipated by extending this time scale and narrowing the signal fringe width. However, the total systematic uncertainty is estimated to be $\sim 6$ parts per $10^6$ (ppm). We describe methods of reducing these systematic errors.Comment: 8 pages, 5 figures, submitted to PR

Racism, anti-racist practice and social work: articulating the teaching and learning experiences of Black social workers

In the mid 1990s a Black practice teacher programme was established in Manchester and Merseyside with the primary aim to increase the number of Black practice teachers in social work organisations, and in turn provide a supportive and encouraging learning environment for Black student social workers whilst on placement. In the north‐west of England research has been undertaken, to establish the quality of the practice teaching and student learning taking place with Black practice teachers and students. This paper is an exploration of the ideas generated within the placement process that particularly focused on the discourse of racism and ant‐racist practice. Black students and practice teachers explain their understanding of racism and anti‐racist practice within social work. From the research, the paper will critique some of the ideas concerning anti‐racism. In particular, it will question whether anti‐racist social work practice needs to be re‐evaluated in the light of a context with new migrants, asylum seekers and refugees. It will concluded, by arguing that whilst the terms anti‐racism, Black and Minority Ethnic have resonance as a form of political strategic essentialism, it is important to develop more positive representations in the future

Organometallic iridium(III) anticancer complexes with new mechanisms of action: NCI-60 screening, mitochondrial targeting, and apoptosis

Platinum complexes related to cisplatin, cis-[PtCl2(NH3)2], are successful anticancer drugs; however, other transition metal complexes offer potential for combating cisplatin resistance, decreasing side effects, and widening the spectrum of activity. Organometallic half-sandwich iridium (IrIII) complexes [Ir(Cpx)(XY)Cl]+/0 (Cpx = biphenyltetramethylcyclopentadienyl and XY = phenanthroline (1), bipyridine (2), or phenylpyridine (3)) all hydrolyze rapidly, forming monofunctional G adducts on DNA with additional intercalation of the phenyl substituents on the Cpx ring. In comparison, highly potent complex 4 (Cpx = phenyltetramethylcyclopentadienyl and XY = N,N-dimethylphenylazopyridine) does not hydrolyze. All show higher potency toward A2780 human ovarian cancer cells compared to cisplatin, with 1, 3, and 4 also demonstrating higher potency in the National Cancer Institute (NCI) NCI-60 cell-line screen. Use of the NCI COMPARE algorithm (which predicts mechanisms of action (MoAs) for emerging anticancer compounds by correlating NCI-60 patterns of sensitivity) shows that the MoA of these IrIII complexes has no correlation to cisplatin (or oxaliplatin), with 3 and 4 emerging as particularly novel compounds. Those findings by COMPARE were experimentally probed by transmission electron microscopy (TEM) of A2780 cells exposed to 1, showing mitochondrial swelling and activation of apoptosis after 24 h. Significant changes in mitochondrial membrane polarization were detected by flow cytometry, and the potency of the complexes was enhanced ca. 5× by co-administration with a low concentration (5 μM) of the γ-glutamyl cysteine synthetase inhibitor L-buthionine sulfoximine (L-BSO). These studies reveal potential polypharmacology of organometallic IrIII complexes, with MoA and cell selectivity governed by structural changes in the chelating ligands

Formal modeling and analysis of cognitive agent behavior

From an external perspective, cognitive agent behavior can be described by specifying (temporal) correlations of a certain complexity between stimuli (input states) and (re)actions (output states) of the agent. From an internal perspective the agent’s dynamics can be characterized by direct (causal) temporal relations between internal and mental states of the agent. The latter type of specifications can be represented in a relatively simple, executable format, which enables different types of analysis of the agent’s behavior. In particular, simulations of the agent’s behavior under different (environmental) circumstances can be explored. Furthermore, by applying verification techniques, automated analysis of the consequences of the agent’s behavior can be carried out. To enable such types of analysis when only given an external behavioral specification, this has to be transformed first into some type of executable format. An automated procedure for such a transformation is proposed in this paper. The application of the transformation procedure is demonstrated for a number of cases, showing examples of the types of analysis as mentioned for different forms of behavior

Demonstration of improved sensitivity of echo interferometers to gravitational acceleration

We have developed two configurations of an echo interferometer that rely on standing wave excitation of a laser-cooled sample of rubidium atoms that measures acceleration. For a two-pulse configuration, the interferometer signal is modulated at the recoil frequency and exhibits a sinusoidal frequency chirp as a function of pulse spacing. For a three-pulse stimulated echo configuration, the signal is observed without recoil modulation and exhibits a modulation at a single frequency. The three-pulse configuration is less sensitive to effects of vibrations and magnetic field curvature leading to a longer experimental timescale. For both configurations of the atom interferometer (AI), we show that a measurement of acceleration with a statistical precision of 0.5% can be realized by analyzing the shape of the echo envelope that has a temporal duration of a few microseconds. Using the two-pulse AI, we obtain measurements of acceleration that are statistically precise to 6 parts per million (ppm) on a 25 ms timescale. Using the three-pulse AI, we obtain measurements of acceleration that are statistically precise to 0.4 ppm on a timescale of 50 ms. A further statistical enhancement is achieved by analyzing the data across the echo envelope to improve the statistical precision to 75 parts per billion (ppb). We discuss methods for reducing prominent systematic effects due to a magnetized vacuum chamber and improving the signal-to-noise ratio. Simulations of both AI configurations with a timescale of 300 ms reached in a non-magnetic vacuum chamber suggest that an optimized experiment with improved vibration isolation and atoms selected in the mF = 0 state can result in measurements of g statistically precise to 0.3 pbb for the two-pulse AI and 0.6 ppb for the three-pulse AI.Comment: 17 pages, 9 figures, 3 table

<em>Enterococcus faecalis</em> Infection Causes Inflammation, Intracellular Oxphos-Independent ROS Production, and DNA Damage in Human Gastric Cancer Cells

Background: Achlorhydria caused by e.g. atrophic gastritis allows for bacterial overgrowth, which induces chronic inflammation and damage to the mucosal cells of infected individuals driving gastric malignancies and cancer. Enterococcus faecalis (E. faecalis) can colonize achlohydric stomachs and we therefore wanted to study the impact of E. faecalis infection on inflammatory response, reactive oxygen species (ROS) formation, mitochondrial respiration, and mitochondrial genetic stability in gastric mucosal cells. Methods: To separate the changes induced by bacteria from those of the inflammatory cells we established an in vitro E. faecalis infection model system using the gastric carcinoma cell line MKN74. Total ROS and superoxide was measured by fluorescence microscopy. Cellular oxygen consumption was characterized non-invasively using XF24 microplate based respirometry. Gene expression was examined by microarray, and response pathways were identified by Gene Set Analysis (GSA). Selected gene transcripts were verified by quantitative real-time polymerase chain reaction (qRT-PCR). Mitochondrial mutations were determined by sequencing. Results: Infection of MKN74 cells with E. faecalis induced intracellular ROS production through a pathway independent of oxidative phosphorylation (oxphos). Furthermore, E. faecalis infection induced mitochondrial DNA instability. Following infection, genes coding for inflammatory response proteins were transcriptionally up-regulated while DNA damage repair and cell cycle control genes were down-regulated. Cell growth slowed down when infected with viable E. faecalis and responded in a dose dependent manner to E. faecalis lysate. Conclusions: Infection by E. faecalis induced an oxphos-independent intracellular ROS response and damaged the mitochondrial genome in gastric cell culture. Finally the bacteria induced an NF-kappa B inflammatory response as well as impaired DNA damage response and cell cycle control gene expression

Towards neuro-inspired symbolic models of cognition: linking neural dynamics to behaviors through asynchronous communications

A computational architecture modeling the relation between perception and action is proposed. Basic brain processes representing synaptic plasticity are first abstracted through asynchronous communication protocols and implemented as virtual microcircuits. These are used in turn to build mesoscale circuits embodying parallel cognitive processes. Encoding these circuits into symbolic expressions gives finally rise to neuro-inspired programs that are compiled into pseudo-code to be interpreted by a virtual machine. Quantitative evaluation measures are given by the modification of synapse weights over time. This approach is illustrated by models of simple forms of behaviors exhibiting cognition up to the third level of animal awareness. As a potential benefit, symbolic models of emergent psychological mechanisms could lead to the discovery of the learning processes involved in the development of cognition. The executable specifications of an experimental platform allowing for the reproduction of simulated experiments are given in “Appendix”

Geology of Rum Cay, Bahamas: A Field Trip Guide

See other Smith authored Field Trip Guides of Gerace Research Centre

The participation of pyridoxal phosphate, carnosine and calcium in the reversal of contraction in muscle fiber systems

A relaxing system requiring phosphocreatine as the relaxant may be restored to glycerinated psoas fibers by incubation in a reaction mixture containing pyridoxal phosphate, calcium, carnosine, magnesium, ATP, and sodium maleate at pH 6.5. The capacity to relax, once established, persists after elution in buffer of the diffusible fraction of the reactants. The relaxation by phosphocreatine is reversed by calcium. The restored relaxing system is also subject to relaxation by the dialyzable fraction of the microsomal-free crude muscle extract, but in this case carnosine is not required in the reaction medium.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32245/1/0000307.pd

Symbolic violence and the invisibility of disability

Disability as a social justice issue is not part of mainstream talk. Approximately 15% of the world’s population has a disability, and yet persons with disabilities are systematically subjected to this sort of exclusion. If considered in terms of social power, then persons with disabilities are the largest single minority group. Amongst minorities, exclusion from the social and representational order is a forceful form of symbolic violence. Persons with disabilities are systematically subjected to this sort of exclusion. In the public domain, persons with disabilities are either not represented at all, or misrepresented. The misrepresentation of persons with disabilities takes a host of cultural forms. This paper explores a few examples of these forms, as they can be considered examples of symbolic violence. We explore how negative social value may be internalised, and how this constitutes a form of symbolic violence experienced by persons with disabilities. We argue that persons with disabilities must constantly act against subtle and blatant acts of symbolic violence – including exclusion – and that the necessity of constant resistance characterises the lives of disabled persons. We argue that it is necessary not only to recognise the detrimental effects of having to confront the symbolic violence of a society which is structured for the benefit of those with typical embodiment, but also to frame this social injustice as something which leads to very real and very dangerous exclusions