Exploring Accelerating Hairy Black Holes in 2+12+1 Dimensions: The Asymptotically Locally Anti-de Sitter Class and its Holography

In the realm of lower-dimensional accelerating spacetimes, it is well-established that the presence of domain walls, which are co-dimension one topological defects, is a necessary condition for their construction. We expand the geometric framework by adding a conformally coupled scalar field. This endeavor leads to the identification of several new families of three-dimensional accelerating spacetimes with asymptotically locally anti-de Sitter (AdS) behavior. Notably, one of these solutions showcases a hairy generalization of the accelerating BTZ black hole. This solution is constructed at both slow and rapid phases of acceleration, and its connection with established vacuum spacetime models is explicitly elucidated. The inclusion of the scalar field imparts a non-constant Ricci curvature to the domain wall, thereby rendering these configurations particularly suitable for the construction of two-dimensional quantum black holes. To establish a well-posed variational principle in the presence of the domain wall, two essential steps are undertaken. First, we extend the conventional renormalized AdS$_3$ action to accommodate the presence of the scalar field. Second, to establish a well-posed variational principle, we extend the renormalized AdS$_3$ action to include the scalar field and incorporate the Gibbons--Hawking--York term for internal boundaries and domain wall tension. We engage in holographic computations, thereby determining the explicit form of the holographic stress tensor. In this context, the stress tensor can be expressed as that of a perfect fluid situated on a curved background. Additionally, it paves the road to ascertain the spacetime mass. Finally, we close by demonstrating the existence of three-dimensional accelerating spacetimes with asymptotically locally flat and asymptotically locally de Sitter geometries, particularly those embodying black holes.Comment: 45 pages, 17 figures, 3 table

Discrete foci containing RNase A are found in nucleoli of HeLa cells aged in culture

We have studied by means of ultrastructural immunocytochemistry the localization of RNase A in nuclei of HeLa cells in control conditions and following cell ageing in culture. We have found that roundish, electron dense foci, which contain a significant amount of RNase A, can be detected within nucleoli of aged cells. These bodies also contain RNA and lack ribosomal S3 proteins, and may represent either simple storage sites or areas where RNA degradation takes place

Aged gastrocnemius muscle of mice positively responds to a late onset adapted physical training

Introduction: A regular physical training is known to contribute to preserve muscle mass and strength, maintaining structure and function of neural and vascular compartments and preventing muscle insulin resistance and inflammation. However, physical activity is progressively reduced during aging causing mobility limitations and poor quality of life. Although physical exercise for rehabilitation purposes (e.g., after fractures or cardiovascular events) or simply aiming to counteract the development of sarcopenia is frequently advised by physicians, nevertheless few data are available on the targets and the global effects on the muscle organ of adapted exercise especially if started at old age.Methods: To contribute answering this question for medical translational purposes, the proteomic profile of the gastrocnemius muscle was analyzed in 24-month-old mice undergoing adapted physical training on a treadmill for 12 weeks or kept under a sedentary lifestyle condition. Proteomic data were implemented by morphological and morphometrical ultrastructural evaluations.Results and Discussion: Data demonstrate that muscles can respond to adapted physical training started at old age, positively modulating their morphology and the proteomic profile fostering protective and saving mechanisms either involving the extracellular compartment as well as muscle cell components and pathways (i.e., mitochondrial processes, cytoplasmic translation pathways, chaperone-dependent protein refolding, regulation of skeletal muscle contraction). Therefore, this study provides important insights on the targets of adapted physical training, which can be regarded as suitable benchmarks for future in vivo studies further exploring the effects of this type of physical activity by functional/metabolic approaches

Effects of mild ozonisation on gene expression and nuclear domains organization in vitro

In the last two decades, the use of ozone (O3) as a complementary medical approach has progressively been increasing; however, its application is still limited due to the numerous doubts about its possible toxicity, despite the low concentrations used in therapy. For an appropriate and safe clinical application of a potentially toxic agent such as O3, it is crucial to elucidate the cellular response to its administration. Molecular analyses and transmission electron microscopy were here combined to investigate in vitro the effects of O3 administration on transcriptional activity and nuclear domains organization of cultured SH-SY5Y neuronal cells; low O3 concentrations were used as those currently administered in clinical practice. Mild ozonisation did not affect cell proliferation or death, while molecular analyses showed an O3-induced modulation of some genes involved in the cell response to stress (HMOX1, ERCC4, CDKN1A) and in the transcription machinery (CTDSP1). Ultrastructural cytochemistry after experiments of bromouridine incorporation consistently demonstrated an increased transcriptional rate at both the nucleoplasmic (mRNA) and the nucleolar (rRNA) level. No ultrastructural alteration of nuclear domains was observed. Our molecular, ultrastructural and cytochemical data demonstrate that a mild toxic stimulus such as mild ozonisation stimulate cell protective pathways and nuclear transcription, without altering cell viability. This could possibly account for the positive effects observed in ozone-treated patients

Insights into Candida tropicalis nosocomial infections and virulence factors

Candida tropicalis is considered the first or the second non-Candida albicans Candida (NCAC) species most frequently isolated from candidosis, mainly in patients admitted in intensive care units (ICUs), especially with cancer, requiring prolonged catheterization, or receiving broad-spectrum antibiotics. The proportion of candiduria and candidemia caused by C. tropicalis varies widely with geographical area and patient group. Actually, in certain countries, C. tropicalis is more prevalent, even compared with C. albicans or other NCAC species. Although prophylactic treatments with fluconazole cause a decrease in the frequency of candidosis caused by C. tropicalis, it is increasingly showing a moderate level of fluconazole resistance. The propensity of C. tropicalis for dissemination and the high mortality associated with its infections might be strongly related to the potential of virulence factors exhibited by this species, such as adhesion to different host surfaces, biofilm formation, infection and dissemination, and enzymes secretion. Therefore, the aim of this review is to outline the present knowledge on all the above-mentioned C. tropicalis virulence traits.The authors acknowledge Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil, for supporting Melyssa Negri (BEX 4642/06-6) and Fundacao para a Ciencia e Tecnologia (FCT), Portugal, for supporting Sonia Silva (SFRH/BPD/71076/2010), and European Community fund FEDER, trough Program COMPETE under the Project FCOMP-01-0124-FEDER-007025 (PTDC/AMB/68393/2006) is gratefully acknowledged

Ryanodine receptor 1 (RYR1) mutations in two patients with tubular aggregate myopathy

Two likely causative mutations in the RYR1 gene were identified in two patients with myopathy with tubular aggregates, but no evidence of cores or core-like pathology on muscle biopsy. These patients were clinically evaluated and underwent routine laboratory investigations, electrophysiologic tests, muscle biopsy and muscle magnetic resonance imaging (MRI). They reported stiffness of the muscles following sustained activity or cold exposure and had serum creatine kinase elevation. The identified RYR1 mutations (p.Thr2206Met or p.Gly2434Arg, in patient 1 and patient 2, respectively) were previously identified in individuals with malignant hyperthermia susceptibility and are reported as causative according to the European Malignant Hyperthermia Group rules. To our knowledge, these data represent the first identification of causative mutations in the RYR1 gene in patients with tubular aggregate myopathy and extend the spectrum of histological alterations caused by mutation in the RYR1 gene

Arquitectura bioclimática: una nueva metodología para abordar la temática

El presente trabajo describe las tareas realizadas por los alumnos durante el desarrollo de la materia electiva “Arquitectura Bioclimática” En base al objetivo general de la materia de “proveer de fundamentos teóricos-practicos; para potenciales agentes de aprovechamiento de energías no convencionales en el diseño y construcción de edificios” , y a la intención de “promover en el alumno una actitud científica y un aprendizaje invcstigativo”, es que se incorpora a la materia una nueva metodología de trabajo, similar a la empleada en el actual proyecto de investigación desarrollado por la cátedra. Los alumnos trabajaron sobre edificios de la Universidad Nacional de Tucumán, analizaron las características climáticas de San Miguel de Tucumán, determinaron el comportamiento térmico, lumínico y ventilante de cada uno de ellos, y teniendo en consideración dichos aspectos, establecieron aciertos y errores en las resoluciones edilicias, y a partir de ello realizaron propuestas de diseño de adecuación del edificio a las condicionantes climáticas de la ciudad. A partir de la metodología de trabajo empleada, los alumnos accedieron, por medio de la auditoría energética de un edificio existente, a los contenidos y métodos de cálculos actualizados de la materia, alcanzando resultados altamente satisfactorios.Tema: Educación en Energías Renovables.Asociación Argentina de Energía Sola

Arquitectura bioclimática: una nueva metodología para abordar la temática

El presente trabajo describe las tareas realizadas por los alumnos durante el desarrollo de la materia electiva “Arquitectura Bioclimática” En base al objetivo general de la materia de “proveer de fundamentos teóricos-practicos; para potenciales agentes de aprovechamiento de energías no convencionales en el diseño y construcción de edificios” , y a la intención de “promover en el alumno una actitud científica y un aprendizaje invcstigativo”, es que se incorpora a la materia una nueva metodología de trabajo, similar a la empleada en el actual proyecto de investigación desarrollado por la cátedra. Los alumnos trabajaron sobre edificios de la Universidad Nacional de Tucumán, analizaron las características climáticas de San Miguel de Tucumán, determinaron el comportamiento térmico, lumínico y ventilante de cada uno de ellos, y teniendo en consideración dichos aspectos, establecieron aciertos y errores en las resoluciones edilicias, y a partir de ello realizaron propuestas de diseño de adecuación del edificio a las condicionantes climáticas de la ciudad. A partir de la metodología de trabajo empleada, los alumnos accedieron, por medio de la auditoría energética de un edificio existente, a los contenidos y métodos de cálculos actualizados de la materia, alcanzando resultados altamente satisfactorios.Tema: Educación en Energías Renovables.Asociación Argentina de Energía Sola

Arquitectura bioclimática: una nueva metodología para abordar la temática

El presente trabajo describe las tareas realizadas por los alumnos durante el desarrollo de la materia electiva “Arquitectura Bioclimática” En base al objetivo general de la materia de “proveer de fundamentos teóricos-practicos; para potenciales agentes de aprovechamiento de energías no convencionales en el diseño y construcción de edificios” , y a la intención de “promover en el alumno una actitud científica y un aprendizaje invcstigativo”, es que se incorpora a la materia una nueva metodología de trabajo, similar a la empleada en el actual proyecto de investigación desarrollado por la cátedra. Los alumnos trabajaron sobre edificios de la Universidad Nacional de Tucumán, analizaron las características climáticas de San Miguel de Tucumán, determinaron el comportamiento térmico, lumínico y ventilante de cada uno de ellos, y teniendo en consideración dichos aspectos, establecieron aciertos y errores en las resoluciones edilicias, y a partir de ello realizaron propuestas de diseño de adecuación del edificio a las condicionantes climáticas de la ciudad. A partir de la metodología de trabajo empleada, los alumnos accedieron, por medio de la auditoría energética de un edificio existente, a los contenidos y métodos de cálculos actualizados de la materia, alcanzando resultados altamente satisfactorios.Tema: Educación en Energías Renovables.Asociación Argentina de Energía Sola