Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera

Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent developments in multiphoton FLIM methods and also present a description of a novel multiphoton FLIM system using a streak camera that was developed in our laboratory. We provide an example of a typical application of the system in which we measure the fluorescence resonance energy transfer between a donor/acceptor pair of fluorescent proteins within a cellular specimen.Comment: Overview of FLIM techniques, StreakFLIM instrument, FRET application

Weyl-Underhill-Emmrich quantization and the Stratonovich-Weyl quantizer

Weyl-Underhill-Emmrich (WUE) quantization and its generalization are considered. It is shown that an axiomatic definition of the Stratonovich-Weyl (SW) quantizer leads to severe difficulties. Quantization on the cylinder within the WUE formalism is discussed.Comment: 15+1 pages, no figure

The Weyl bundle as a differentiable manifold

Construction of an infinite dimensional differentiable manifold ${\mathbb R}^{\infty}$ not modelled on any Banach space is proposed. Definition, metric and differential structures of a Weyl algebra and a Weyl algebra bundle are presented. Continuity of the $\circ$-product in the Tichonov topology is proved. Construction of the $*$-product of the Fedosov type in terms of theory of connection in a fibre bundle is explained.Comment: 31 pages; revised version - some typoes have been eliminated, notation has been simplifie

The rigged Hilbert space approach to the Lippmann-Schwinger equation. Part II: The analytic continuation of the Lippmann-Schwinger bras and kets

The analytic continuation of the Lippmann-Schwinger bras and kets is obtained and characterized. It is shown that the natural mathematical setting for the analytic continuation of the solutions of the Lippmann-Schwinger equation is the rigged Hilbert space rather than just the Hilbert space. It is also argued that this analytic continuation entails the imposition of a time asymmetric boundary condition upon the group time evolution, resulting into a semigroup time evolution. Physically, the semigroup time evolution is simply a (retarded or advanced) propagator.Comment: 32 pages, 3 figure

Deformation Quantization of Bosonic Strings

Deformation quantization of bosonic strings is considered. We show that the light-cone gauge is the most convenient classical description to perform the quantization of bosonic strings in the deformation quantization formalism. Similar to the field theory case, the oscillator variables greatly facilitates the analysis. The mass spectrum, propagators and the Virasoro algebra are finally described within this deformation quantization scheme.Comment: 33+1 pages, harvmac file, no figure

The rigged Hilbert space approach to the Lippmann-Schwinger equation. Part I

We exemplify the way the rigged Hilbert space deals with the Lippmann-Schwinger equation by way of the spherical shell potential. We explicitly construct the Lippmann-Schwinger bras and kets along with their energy representation, their time evolution and the rigged Hilbert spaces to which they belong. It will be concluded that the natural setting for the solutions of the Lippmann-Schwinger equation--and therefore for scattering theory--is the rigged Hilbert space rather than just the Hilbert space.Comment: 34 pages, 1 figur

Quantum Mechanics on the cylinder

A new approach to deformation quantization on the cylinder considered as phase space is presented. The method is based on the standard Moyal formalism for R^2 adapted to (S^1 x R) by the Weil--Brezin--Zak transformation. The results are compared with other solutions of this problem presented by Kasperkovitz and Peev (Ann. Phys. vol. 230, 21 (1994)0 and by Plebanski and collaborators (Acta Phys. Pol. vol. B 31}, 561 (2000)). The equivalence of these three methods is proved.Comment: 21 pages, LaTe

Phase-space formulation of quantum mechanics and quantum state reconstruction for physical systems with Lie-group symmetries

We present a detailed discussion of a general theory of phase-space distributions, introduced recently by the authors [J. Phys. A {\bf 31}, L9 (1998)]. This theory provides a unified phase-space formulation of quantum mechanics for physical systems possessing Lie-group symmetries. The concept of generalized coherent states and the method of harmonic analysis are used to construct explicitly a family of phase-space functions which are postulated to satisfy the Stratonovich-Weyl correspondence with a generalized traciality condition. The symbol calculus for the phase-space functions is given by means of the generalized twisted product. The phase-space formalism is used to study the problem of the reconstruction of quantum states. In particular, we consider the reconstruction method based on measurements of displaced projectors, which comprises a number of recently proposed quantum-optical schemes and is also related to the standard methods of signal processing. A general group-theoretic description of this method is developed using the technique of harmonic expansions on the phase space.Comment: REVTeX, 18 pages, no figure

On two superintegrable nonlinear oscillators in N dimensions

We consider the classical superintegrable Hamiltonian system given by $H=T+U={p^2}/{2(1+\lambda q^2)}+{{\omega}^2 q^2}/{2(1+\lambda q^2)}$, where U is known to be the "intrinsic" oscillator potential on the Darboux spaces of nonconstant curvature determined by the kinetic energy term T and parametrized by {\lambda}. We show that H is Stackel equivalent to the free Euclidean motion, a fact that directly provides a curved Fradkin tensor of constants of motion for H. Furthermore, we analyze in terms of {\lambda} the three different underlying manifolds whose geodesic motion is provided by T. As a consequence, we find that H comprises three different nonlinear physical models that, by constructing their radial effective potentials, are shown to be two different nonlinear oscillators and an infinite barrier potential. The quantization of these two oscillators and its connection with spherical confinement models is briefly discussed.Comment: 11 pages; based on the contribution to the Manolo Gadella Fest-60 years-in-pucelandia, "Recent advances in time-asymmetric quantum mechanics, quantization and related topics" hold in Valladolid (Spain), 14-16th july 201

Relativistic resonances: Their masses, widths, lifetimes, superposition, and causal evolution

Whether one starts form the analytic S-matrix definition or the requirement of gauge parameter independence in renormalization theory, a relativistic resonance is given by a pole at a complex value s of energy squared. The complex number s does not define the mass and width separately and this definition does not lead to interfering Breit-Wigner if two or more resonances are involved. To accomplish both we invoke the decaying particle aspect of a resonance and associate to each pole a space of relativistic Gamow kets which transform irreducibly under causal Poincare transformations. A Gamow state has an exponential time evolution and one can choose of the many possible width parameters, that parameter as the width of the relativistic resonance which equals the inverse lifetime. This uniquely defines the mass and width parameters for a relativistic resonance. Two or more poles in the same partial wave are given by the sum of Breit-Wigners in the scattering amplitude and by a superposition of Gamow vectors with each Gamow vector corresponding to one Breit-Wigner. In addition to the sum of Breit-Wigners the scattering amplitude contains a background amplitude representing direct production of the final state (contact terms).This contact amplitude is associated to a background vector which is a continuous superposition of Lippmann-Schwinger states. Omitting this continuum gives the Weisskopf-Wigner approximation.Comment: 22 pages, REVTe