Alien Registration- Gilley, Earl B. (Seal Harbor, Hancock County)

https://digitalmaine.com/alien_docs/19947/thumbnail.jp

Crowding Promotes the Switch from Hairpin to Pseudoknot Conformation in Human Telomerase RNA

Formation of a pseudoknot in the conserved RNA core domain in the ribonucleoprotein human telomerase is required for function. In vitro experiments show that the pseudoknot (PK) is in equilibrium with an extended hairpin (HP) structure. We use molecular simulations of a coarse-grained model, which reproduces most of the salient features of the experimental melting profiles of PK and HP, to show that crowding enhances the stability of PK relative to HP in the wild type and in a mutant associated with dyskeratosis congenita. In monodisperse suspensions, small crowding particles increase the stability of compact structures to a greater extent than larger crowders. If the sizes of crowders in a binary mixture are smaller than the unfolded RNA, the increase in melting temperature due to the two components is additive. In a ternary mixture of crowders that are larger than the unfolded RNA, which mimics the composition of ribosome, large enzyme complexes and proteins in E. coli, the marginal increase in stability is entirely determined by the smallest component. We predict that crowding can restore partially telomerase activity in mutants, which dramatically decrease the PK stability.Comment: File "JACS_MAIN_archive_PDF_from_DOC.pdf" (PDF created from DOC) contains the main text of the paper File JACS_SI_archive.tex + 7 figures are the supplementary inf

Bird Friendly Beef: Exploring the Impacts of Regenerative Forage Production

In the eastern US, managed hayfields and pasturelands represent a significant portion of remaining available grassland bird habitat, hosting several declining species including the eastern meadowlark (Sturnella magna) and the grasshopper sparrow (Ammodramus savannarum). However, these working landscapes have deteriorated in their ability to support grassland birds in recent decades due to more frequent and early hay cuttings and intensive grazing. Since the conservation of grassland birds is inextricably linked to agricultural systems, for conservation to be effective, land management must aim to benefit both producers and birds. Regenerative agriculture is an emerging approach that incorporates land management practices that benefit agricultural ecosystems and increase efficiency and profitability. Regenerative grazing has demonstrated associations with greater bird abundance, but there is a significant gap in research that investigates whether these practices contribute to successful nesting. Collaborators from Smithsonian’s Virginia Working Landscapes, American Farmland Trust, and George Mason University set out to explore the effects of regenerative grazing practices on forage quality and the reproductive success of grassland birds. We established four experimental grazing and haying treatments on private working lands. For each treatment, we collected forage samples for nutrient analysis and quantified reproductive success using a combination of territory mapping and systematic nest searching. Our preliminary analysis suggests that grazing pastures early and stockpiling for fall and winter grazing results in standing forages suitable in quality for several different production classes of livestock while reducing producer reliance on harvested feeds such as hay. Our observations also identified this system to provide the greatest propensity for fledgling success of grassland birds due to the lack of disturbances during peak nesting periods which traditionally are due to trampling, haying, and predation

Accumulation and Release of Nutrients by Immersed Stalks Collected on Selected Dates Following Harvest

The concentrations of phosphorus (P) and nitrogen (N) in runoff from cropland areas may be influenced by accumulation and release of P and N by stalk residues. A laboratory study was conducted to measure the effects of time since harvest and immersion period on accumulation and release of P and N by corn, soybean, and wheat stalks. Experimental variables included type of stalk material (corn, soybean, and wheat), time since harvest (six residue collection dates over an approximate 1-year period), and stalk immersion period (25 s (0.42 min), 250 s (4.2 min), 2500 s (42 min), 25,000 s (6.9 h), and 86,400 s (24 h)). The initial concentration of each of the P and N constituents in a test solution was 6 μg mL−1. The soybean, wheat, and corn residue released PO4-P at mean rates of 40, 69, and 141 μg g−1 residue, respectively. The amount of PO4-P that was released consistently increased as immersion period became greater. Corn and wheat residue either accumulated or released NO3-N depending on residue collection date. Soybean residue accumulated an average of 20 μg NO3-N g−1 residue. Wheat residue obtained on five of the collection dates accumulated an average of 13 μgNO3-N g−1 residue. Residue collection date also influenced accumulation of NH4-N by soybean and wheat residue. Corn residue released an average of 77 μg NH4-N g−1 residue. The type of crop residue material, the amount of time the residue has remained in the field following harvest, and residue immersion period were found to influence nutrient concentrations of solution

Introduction: Ideology, propaganda, and political discourse in the Xi Jinping era

The ideology, propaganda, and political discourse of the Communist Party of China (CPC) have continued to function as key elements of the political system of the People’s Republic of China (PRC) in the post-Maoist period since 1978. In the first term of the Xi Jinping leadership (2012–2017), the CPC, for instance, elaborated on its guiding ideological concepts, devised inventive ideational framings of phenomena usually perceived as tangible (such as the BNew Normal^), engaged in complex intellectual debates on crucial topics (such as Beco-civilization^), intensified and diversified its argumentation patterns and discursive strategies, and consolidated ideational governance over some citizens’ individual values, beliefs, and loyalties. Furthermore, it is often no longer possible to differentiate between the CPC’s internal and external propaganda, as seemingly exclusively domestic ideational and discursive issues increasingly correlate with international phenomena. However, the trends in the Xi era do not present paradigmatic shifts, but rather an overall reassertion-cuminnovation of previous Maoist and post-Maoist uses of ideology, propaganda, and political discourse, primarily aiming at strengthening one-party rule

Trim17, novel E3 ubiquitin-ligase, initiates neuronal apoptosis

Accumulating data indicate that the ubiquitin-proteasome system controls apoptosis by regulating the level and the function of key regulatory proteins. In this study, we identified Trim17, a member of the TRIM/RBCC protein family, as one of the critical E3 ubiquitin ligases involved in the control of neuronal apoptosis upstream of mitochondria. We show that expression of Trim17 is increased both at the mRNA and protein level in several in vitro models of transcription-dependent neuronal apoptosis. Expression of Trim17 is controlled by the PI3K/Akt/GSK3 pathway in cerebellar granule neurons (CGN). Moreover, the Trim17 protein is expressed in vivo, in apoptotic neurons that naturally die during post-natal cerebellar development. Overexpression of active Trim17 in primary CGN was sufficient to induce the intrinsic pathway of apoptosis in survival conditions. This pro-apoptotic effect was abolished in Bax(-/-) neurons and depended on the E3 activity of Trim17 conferred by its RING domain. Furthermore, knock-down of endogenous Trim17 and overexpression of dominant-negative mutants of Trim17 blocked trophic factor withdrawal-induced apoptosis both in CGN and in sympathetic neurons. Collectively, our data are the first to assign a cellular function to Trim17 by showing that its E3 activity is both necessary and sufficient for the initiation of neuronal apoptosis. Cell Death and Differentiation (2010) 17, 1928-1941; doi: 10.1038/cdd.2010.73; published online 18 June 201

Narrow Grass Hedge Effects on Nutrient Transport Following Compost Application

The placement of stiff‐stemmed grass hedges on the contour along a hillslope has been shown to decrease nutrient transport in runoff. This study was conducted to measure the effectiveness of a narrow grass hedge in reducing runoff nutrient transport from plots with a range of soil nutrient values. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha-1 to a silty clay loam soil and then incorporated by disking. Soil samples were collected 243 days later for analysis of water‐soluble phosphorus (WSP), Bray and Kurtz No. 1 phosphorus (Bray‐1 P), NO3-N, and NH4-N. Three 30 min simulated rainfall events, separated by 24 h intervals, were then applied. The transport of dissolved phosphorus (DP), total P (TP), NO3-N, NH4-N, total nitrogen (TN), runoff, and soil erosion were measured from 0.75 m wide × 4.0 m long plots. Compost application rate significantly affected soil measurements of WSP, Bray‐1 P, and NO3-N content. The transport of DP, TP, NO3-N, NH4-N, TN, runoff, and soil erosion was reduced significantly on the plots with a grass hedge. Mean runoff rates on the hedge and no‐hedge treatments were 17 and 29 mm, and erosion rates were 0.12 and 1.46 Mg ha-1, respectively. Compost application rate significantly affected the transport of DP, TP, and NO3-N in runoff. The experimental results indicate that stiff‐stemmed grass hedges, planted at selected downslope intervals, can significantly reduce the transport of nutrients in runoff from areas with a range of soil nutrient values

Leonard B. Gilley Correspondence

Entries include letters on Farmington State College stationery, poems written by Gilley and his wife together and separately, and a biography. Date range: 1970-04/1970-0

Temporal Changes in Nutrient Transport Following Land Application of Manure

Little information is currently available concerning temporal changes in nutrient transport following the addition of manure to cropland areas. This study was conducted to measure nutrient transport in runoff as affected by tillage and time following the application of beef cattle or swine manure to a site on which corn [Zea mays (L.)] was grown. Rainfall simulation tests were initiated 4, 32, 62, 123, and 354 days following land application. Three 30-min simulated rainfall events, separated by 24-hour intervals, were conducted at an intensity of approximately 70 mm hr-1. Dissolved phosphorus (DP), particulate phosphorus (PP), total phosphorus (TP), NO3-N, NH4-N, total nitrogen (TN), electrical conductivity (EC), and pH were measured from 0.75-m wide by 2-m long plots. Concentrations of DP, TP, and NH4-N, in general, declined throughout the year on both the no-till cattle and no-till swine manure treatments. Tillage did not significantly affect concentrations of DP, PP, TP, NH4-N or pH on the swine manure treatments, but significant variations in these variables were measured over time. Under no-till and tilled conditions on both the cattle and swine manure treatments, the smallest concentrations of DP, NO3-N, NH4-N, and TN occurred on the final test date. The increase in pH of runoff during the study is attributed to the addition of CaCO3 to the rations of beef cattle and swine. Tillage appeared to have less of an impact on runoff nutrient transport from cropland areas than length of time since manure application

Temporal Changes in Nutrient Transport Following Land Application of Manure

Little information is currently available concerning temporal changes in nutrient transport following the addition of manure to cropland areas. This study was conducted to measure nutrient transport in runoff as affected by tillage and time following the application of beef cattle or swine manure to a site on which corn [Zea mays (L.)] was grown. Rainfall simulation tests were initiated 4, 32, 62, 123, and 354 days following land application. Three 30-min simulated rainfall events, separated by 24-hour intervals, were conducted at an intensity of approximately 70 mm hr-1. Dissolved phosphorus (DP), particulate phosphorus (PP), total phosphorus (TP), NO3-N, NH4-N, total nitrogen (TN), electrical conductivity (EC), and pH were measured from 0.75-m wide by 2-m long plots. Concentrations of DP, TP, and NH4-N, in general, declined throughout the year on both the no-till cattle and no-till swine manure treatments. Tillage did not significantly affect concentrations of DP, PP, TP, NH4-N or pH on the swine manure treatments, but significant variations in these variables were measured over time. Under no-till and tilled conditions on both the cattle and swine manure treatments, the smallest concentrations of DP, NO3-N, NH4-N, and TN occurred on the final test date. The increase in pH of runoff during the study is attributed to the addition of CaCO3 to the rations of beef cattle and swine. Tillage appeared to have less of an impact on runoff nutrient transport from cropland areas than length of time since manure application