Ferromagnetic (Ga,Mn)N epilayers versus antiferromagnetic GaMn3_3N clusters

Mn-doped wurtzite GaN epilayers have been grown by nitrogen plasma-assisted molecular beam epitaxy. Correlated SIMS, structural and magnetic measurements show that the incorporation of Mn strongly depends on the conditions of the growth. Hysteresis loops which persist at high temperature do not appear to be correlated to the presence of Mn. Samples with up to 2% Mn are purely substitutional Ga$_{1-x}$Mn$_x$N epilayers, and exhibit paramagnetic properties. At higher Mn contents, precipitates are formed which are identified as GaMn$_3$N clusters by x-ray diffraction and absorption: this induces a decrease of the paramagnetic magnetisation. Samples co-doped with enough Mg exhibit a new feature: a ferromagnetic component is observed up to $T_c\sim175$ K, which cannot be related to superparamagnetism of unresolved magnetic precipitates.Comment: Revised versio

Electric-field-induced coherent coupling of the exciton states in a single quantum dot

The signature of coherent coupling between two quantum states is an anticrossing in their energies as one is swept through the other. In single semiconductor quantum dots containing an electron-hole pair the eigenstates form a two-level system that can be used to demonstrate quantum effects in the solid state, but in all previous work these states were independent. Here we describe a technique to control the energetic splitting of these states using a vertical electric field, facilitating the observation of coherent coupling between them. Near the minimum splitting the eigenstates rotate in the plane of the sample, being orientated at 45{\deg} when the splitting is smallest. Using this system we show direct control over the exciton states in one quantum dot, leading to the generation of entangled photon pairs

Extreme genomic erosion after recurrent demographic bottlenecks in the highly endangered Iberian lynx

Background: Genomic studies of endangered species provide insights into their evolution and demographic history, reveal patterns of genomic erosion that might limit their viability, and offer tools for their effective conservation. The Iberian lynx (Lynx pardinus) is the most endangered felid and a unique example of a species on the brink of extinction. Results: We generate the first annotated draft of the Iberian lynx genome and carry out genome-based analyses of lynx demography, evolution, and population genetics. We identify a series of severe population bottlenecks in the history of the Iberian lynx that predate its known demographic decline during the 20th century and have greatly impacted its genome evolution. We observe drastically reduced rates of weak-to-strong substitutions associated with GC-biased gene conversion and increased rates of fixation of transposable elements. We also find multiple signatures of genetic erosion in the two remnant Iberian lynx populations, including a high frequency of potentially deleterious variants and substitutions, as well as the lowest genome-wide genetic diversity reported so far in any species. Conclusions: The genomic features observed in the Iberian lynx genome may hamper short- and long-term viability through reduced fitness and adaptive potential. The knowledge and resources developed in this study will boost the research on felid evolution and conservation genomics and will benefit the ongoing conservation and management of this emblematic species

Strategies to improve the energy efficiency of pressurized water systems

[EN] As time goes by, the need to move water is greater and this water will be pressurized. Layout flexibility, security, quality care, control, lower environmental impact and higher efficiency justify pressurized transport rather than natural gravitational water transport. On the negative side, we find the enormous amount of energy pressurized systems require with the associated negative economic and environmental impacts. Therefore, it is crucial to minimize these impacts and that only can be achieved by improving the energy efficiency of these systems. To achieve that final goal, the first step is to perform an assessment to estimate the margin of improvement from the actual performance of the system to the maximum achievable level of efficiency [1]. The second step is to perform an energy audit in order to identify exactly how the energy is used and where it is lost [2], with the third step being identification of the different actions that can be implemented in practice in a system. The final step is to perform the cost benefit analysis of the selected actions to prioritize execution. The focus of attention of this paper is on the third step, actions that can be classified in operational actions (do not require investments) and structural actions (require investments).Cabrera Marcet, E.; Gomez Selles, E.; Espert Alemany, VB.; Cabrera Rochera, E. (2017). Strategies to improve the energy efficiency of pressurized water systems. Procedia Engineering. 186:294-302. doi:10.1016/j.proeng.2017.03.248S29430218

A Mark in Time Saves Nein

A method for predicting political interactions and policy outcomes based on two political theorems is presented and illustrated with an examination of the decision to merge the two German currencies. Political perceptions and actions are anticipated by combining the substantive knowledge of area experts with the theoretical insights embedded in the median voter theorem and a monotonicity theorem that links expectations to probabilistic statements of action. The proposed model has proven accurate about 90 percent of the time. The proposed forecasting method identifies a sequential strategy that may have been followed by Chancellor Kohl in forging the coalition needed to merge successfully the two German currencies. Using comparative statics, the analysis suggests how subtle and sophisticated Chancellor Kohl had to be to succeed in getting the policy outcome he desired despite stiff opposition.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66924/2/10.1177_019251219201300106.pd

Defects in Meiotic Recombination Delay Progression Through Pachytene in Tex19.1-/- Mouse Spermatocytes

Recombination, synapsis, chromosome segregation and gene expression are co-ordinately regulated during meiosis to ensure successful execution of this specialised cell division. Studies with multiple mutant mouse lines have shown that mouse spermatocytes possess quality control checkpoints that eliminate cells with persistent defects in chromosome synapsis. In addition, studies on Trip13 mod/mod mice suggest that pachytene spermatocytes that successfully complete chromosome synapsis can undergo meiotic arrest in response to defects in recombination. Here, we present additional support for a meiotic recombination-dependent checkpoint using a different mutant mouse line, Tex19.1 −/− . The appearance of early recombination foci is delayed in Tex19.1 −/− spermatocytes during leptotene/zygotene, but some Tex19.1 −/− spermatocytes still successfully synapse their chromosomes and we show that these spermatocytes are enriched for early recombination foci. Furthermore, we show that patterns of axis elongation, chromatin modifications and histone H1t expression are also all co-ordinately skewed towards earlier substages of pachytene in these autosomally synapsed Tex19.1 −/− spermatocytes. We also show that this skew towards earlier pachytene substages occurs in the absence of elevated spermatocyte death in the population, that spermatocytes with features of early pachytene are present in late stage Tex19.1 −/− testis tubules and that the delay in histone H1t expression in response to loss of Tex19.1 does not occur in a Spo11 mutant background. Taken together, these data suggest that a recombination-dependent checkpoint may be able to modulate pachytene progression in mouse spermatocytes to accommodate some types of recombination defect

Energy Assessment of Pressurized Water Systems

[EN] This paper presents three new indicators for assessing the energy efficiency of a pressurized water system and the potential energy savings relative to the available technology and economic framework. The first two indicators are the ideal and real efficiencies of the system and reflect the values of the minimum energy required by users the minimum amount of energy to be supplied to the system (because of its ideal behavior) and the actual energy consumed. The third indicator is the energy performance target, and it is estimated by setting an ambitious but achievable level of energy loss attributable to inefficiencies in the system (e.g., pumping stations, leakage, friction loss). The information provided by these three key performance indicators can make a significant contribution towards increasing system efficiency. The real efficiency indicator shows the actual performance of the system; the energy performance target provides a realistic goal on how the system should be performing; and finally, the ideal efficiency provides the maximum and unachievable level of efficiency (limited by the topographic energy linked to the network topography). The applicability and usefulness of these metrics will be demonstrated with an application in a real case study.The authors acknowledge the very valuable contributions made by the reviewers of this paper, because their comments and suggestions have helped to significantly improve the contents. Additionally, we thank the staff of Aguas de Valencia for providing helpful advice and real case studies used to tune the software tool developed based on this paper. And last but not least, the research leading to these results received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement number 265122. The translation of this paper was funded by the Universitat Politècnica de València, Spain.Cabrera Marcet, E.; Gomez Selles, E.; Cabrera Rochera, E.; Soriano Olivares, J.; Espert Alemany, VB. (2014). Energy Assessment of Pressurized Water Systems. Journal of Water Resources Planning and Management. 141(8):1-12. https://doi.org/10.1061/(ASCE)WR.1943-5452.0000494S112141

Genome of the red alga Porphyridium purpureum

The limited knowledge we have about red algal genomes comes from the highly specialized extremophiles, Cyanidiophyceae. Here, we describe the first genome sequence from a mesophilic, unicellular red alga, Porphyridium purpureum. The 8,355 predicted genes in P. purpureum, hundreds of which are likely to be implicated in a history of horizontal gene transfer, reside in a genome of 19.7 Mbp with 235 spliceosomal introns. Analysis of light-harvesting complex proteins reveals a nuclear-encoded phycobiliprotein in the alga. We uncover a complex set of carbohydrate-active enzymes, identify the genes required for the methylerythritol phosphate pathway of isoprenoid biosynthesis, and find evidence of sexual reproduction. Analysis of the compact, function-rich genome of P. purpureum suggests that ancestral lineages of red algae acted as mediators of horizontal gene transfer between prokaryotes and photosynthetic eukaryotes, thereby significantly enriching genomes across the tree of photosynthetic life

miR-199a-5p Is Upregulated during Fibrogenic Response to Tissue Injury and Mediates TGFbeta-Induced Lung Fibroblast Activation by Targeting Caveolin-1

As miRNAs are associated with normal cellular processes, deregulation of miRNAs is thought to play a causative role in many complex diseases. Nevertheless, the precise contribution of miRNAs in fibrotic lung diseases, especially the idiopathic form (IPF), remains poorly understood. Given the poor response rate of IPF patients to current therapy, new insights into the pathogenic mechanisms controlling lung fibroblasts activation, the key cell type driving the fibrogenic process, are essential to develop new therapeutic strategies for this devastating disease. To identify miRNAs with potential roles in lung fibrogenesis, we performed a genome-wide assessment of miRNA expression in lungs from two different mouse strains known for their distinct susceptibility to develop lung fibrosis after bleomycin exposure. This led to the identification of miR-199a-5p as the best miRNA candidate associated with bleomycin response. Importantly, miR-199a-5p pulmonary expression was also significantly increased in IPF patients (94 IPF versus 83 controls). In particular, levels of miR-199a-5p were selectively increased in myofibroblasts from injured mouse lungs and fibroblastic foci, a histologic feature associated with IPF. Therefore, miR-199a-5p profibrotic effects were further investigated in cultured lung fibroblasts: miR-199a-5p expression was induced upon TGFβ exposure, and ectopic expression of miR-199a-5p was sufficient to promote the pathogenic activation of pulmonary fibroblasts including proliferation, migration, invasion, and differentiation into myofibroblasts. In addition, we demonstrated that miR-199a-5p is a key effector of TGFβ signaling in lung fibroblasts by regulating CAV1, a critical mediator of pulmonary fibrosis. Remarkably, aberrant expression of miR-199a-5p was also found in unilateral ureteral obstruction mouse model of kidney fibrosis, as well as in both bile duct ligation and CCl4-induced mouse models of liver fibrosis, suggesting that dysregulation of miR-199a-5p represents a general mechanism contributing to the fibrotic process. MiR-199a-5p thus behaves as a major regulator of tissue fibrosis with therapeutic potency to treat fibroproliferative diseases. © 2013 Lino Cardenas et al