Checkpoint proteins come under scrutiny

Details are emerging of the interactions between the kinetochore and various spindle checkpoint proteins that ensure that sister chromatids are equally divided between daughter cells during cell division

Active noise control on high frequency narrow band dental drill noise: Preliminary results

Dental drills produce a characteristic noise that is uncomfortable for patients and is also known to be harmful to dentists under prolonged exposure. It is therefore desirable to protect the patient and dentist whilst allowing two-way communication. A solution is to use a combination of the three main noise cancellation methods, namely, Passive Noise Control, Adaptive Filtering and Active Noise Control. Dental drill noise occurs at very high frequency ranges in relation to conventional ANC, typically 2kHz to 6kHz and it has a narrow band characteristic due to the direct relation of the noise to the rotational speed of the bearing. This paper presents a design of an experimental rig where first applications of ANC on dental drill noise are executed using the standard filtered reference Least Mean Square (FXLMS) algorithm. The secondary path is kept as simple as possible, due to the high frequency range of interest, and hence is chosen as the space between headphone loudspeaker and error microphone placed in the ear (input of the headphone loudspeaker and the output of the error microphone). A standard headphone loudspeaker is used for the control source and the microphone inside of an “Ear and Cheek Simulator Type 43AG” is used as the error microphone. The secondary path transfer function is obtained and preliminary results of the application of ANC are discussed

Active noise control for high frequencies

There are many applications that can benefit from Active Noise Control (ANC) such as in aircraft cabins and air conditioning ducts, i.e. in situations where technology interferes with human hearing in a harmful way or disrupts communication. Headsets with analogue ANC circuits have been used in the armed forces for attenuating frequencies below 1 kHz, which when combined with passive filtering offers protection across the whole frequency range of human hearing. A dental surgery is also a noisy environment; in which dental drill noise is commonly off-putting for many patients and is believed to harm the dentist’s hearing over a long period of time. However, dealing with dental drill noise is a different proposition from the applications mentioned above as the frequency range of the peak amplitudes goes from approximately 1.5 kHz to 12 kHz, whereas conventional ANC applications consider a maximum of 1.5 kHz. This paper will review the application of ANC at low frequencies and justify an approach for dealing with dental noise using digital technologies at higher frequencies. The limits of current ANC technologies will be highlighted and the means of improving performance for this dental application will be explored. In particular, technicalities of implementing filtering algorithms on a Digital Signal Processor will be addressed

Real-time adaptive filtering of dental drill noise using a digital signal processor

The application of noise reduction methods requires the integration of acoustics engineering and digital signal processing, which is well served by a mechatronic approach as described in this paper. The Normalised Least Mean Square (NLMS) algorithm is implemented on the Texas Instruments TMS320C6713 DSK Digital Signal Processor (DSP) as an adaptive digital filter for dental drill noise. Blocks within the Matlab/Simulink Signal Processing Blockset and the Embedded Target for TI C6000 DSP family are used. A working model of the algorithm is then transferred to the Code Composer Studio (CCS), where the desired code can be linked and transferred to the target DSP. The experimental rig comprises a noise reference microphone, a microphone for the desired signal, the DSK and loudspeakers. Different load situations of the dental drill are considered as the noise characteristics change when the drill load changes. The result is that annoying drill noise peaks, which occur in a frequency range from 1.5 kHz to 10 kHz, are filtered out adaptively by the DSP. Additionally a schematic design for its implementation in a dentist’s surgery will also be presented

Accurate simulations of the interplay between process and statistical variability for nanoscale FinFET-based SRAM cell stability

In this paper we illustrate how by using advanced atomistic TCAD tools the interplay between long-range process variation and short-range statistical variability in FinFETs can be accurately modelled and simulated for the purposes of Design-Technology Co-Optimization (DTCO). The proposed statistical simulation and compact modelling methodology is demonstrated via a comprehensive evaluation of the impact of FinFET variability on SRAM cell stability

Latrunculin A delays anaphase onset in fission yeast by disrupting an ase1-independent pathway controlling mitotic spindle stability

It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast. However, we show that {Delta}mto1 cells, which are defective in nucleation of cytoplasmic microtubules, have profoundly misoriented spindles but are not delayed in the timing of sister chromatid separation, providing compelling evidence that fission yeast does not possess a spindle orientation checkpoint. Instead, we show that latrunculin A delays anaphase onset by disrupting interpolar microtubule stability. This effect is abolished in a latrunculin A-insensitive actin mutant and exacerbated in cells lacking Ase1, which cross-links antiparallel interpolar microtubules at the spindle midzone both before and after anaphase. These data indicate that both Ase1 and an intact actin cytoskeleton are required for preanaphase spindle stability. Finally, we show that loss of Ase1 activates a checkpoint that requires only the Mad3, Bub1, and Mph1, but not Mad1, Mad2, or Bub3 checkpoint proteins

Modified sorting technique to mitigate the collateral mortality of trawled school prawns (Metapenaeus macleayi)

The potential for changes to onboard handling practices in order to improve the fate of juvenile school prawns (Metapenaeus macleayi) discarded during trawling were investigated in two Australian rivers (Clarence and Hunter) by comparing a purpose-built, water-filled sorting tray against a conventional dry tray across various conditions, including the range of typical delays before the start of sorting the catch (2 min vs. 15 min). Juvenile school prawns (n= 5760), caught during 32 and 16 deployments in each river, were caged and sacrificed at four times: immediately (T0), and at 24 (T24), 72 (T72), and 120 (T12 0) hours after having been discarded. In both rivers, most mortalities occurred between T0 and T24 and, after adjusting for control deaths (<12%), were greatest for the 15-min conventional treatment (up to 41% at T120). Mixed-effects logistic models revealed that in addition to the sampling time, method of sorting, and delay in sorting, the weight of the catch, salinity, and percentage cloud cover were significant predictors of mortality. Although trawling caused some mortalities and comparable stress (measured as L -lactate) in all school prawns, use of the water tray lessened the negative impacts of some of the above factors across both the 2-min and 15-min delays in sorting so that the overall discard mortality was reduced by more than a third. When used in conjunction with selective trawls, widespread application of the water tray should help to improve the sustainability of trawling for school prawns

Dental drill noise reduction using a combination of active noise control, passive noise control and adaptive filtering

Dental drills produce a characteristic high frequency, narrow band noise that is uncomfortable for patients and is also known to be harmful to dentists under prolonged exposure. It is therefore desirable to protect the patient and dentist whilst allowing two-way communication. A solution is to use a combination of the three main noise control methods, namely, Passive Noise Control (PNC), Adaptive Filtering (AF) and Active Noise Control (ANC). This paper discusses the application of the three methods to reduce dental drill noise while allowing two-way communication. Experimental setup for measuring the noise reduction by PNC is explained and results from different headphones and headphone types are presented. The implementation and results of an AF system using the Least Mean Square (LMS) algorithm are shown. ANC requires a modification of the LMS algorithm due to the introduction of the electro-acoustical cancellation path transfer function to compensate for the delays introduced by the control system. Therefore a cancellation path transfer function modeling method based on the filtered reference LMS (FXLMS) algorithm is presented along with preliminary results of the implementation

Meiotic nuclear divisions in budding yeast require PP2ACdc55-mediated antagonism of Net1 phosphorylation by Cdk

During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication. The precise mechanism of how FEAR regulates meiosis is not understood. In this paper, we report that premature activation of FEAR during meiosis caused by loss of protein phosphatase PP2ACdc55 activity blocks bipolar spindle assembly and nuclear divisions. In cdc55 meiotic null (cdc55-mn) cells, the cyclin-dependent kinase (Cdk)–counteracting phosphatase Cdc14 was released prematurely from the nucleolus concomitant with hyperphosphorylation of its nucleolar anchor protein Net1. Crucially, a mutant form of Net1 that lacks six Cdk phosphorylation sites rescued the meiotic defect of cdc55-mn cells. Expression of a dominant mutant allele of CDC14 mimicked the cdc55-mn phenotype. We propose that phosphoregulation of Net1 by PP2ACdc55 is essential for preventing precocious exit from meiosis I

Data management of nanometre­ scale CMOS device simulations

In this paper we discuss the problems arising in managing and curating the data generated by simulations of nanometre scale CMOS (Complementary Metal–Oxide Semiconductor) transistors, circuits and systems and describe the software and operational techniques we have adopted to address them. Such simulations pose a number of challenges including, inter alia, multi­TByte data volumes, complex datasets with complex inter-relations between datasets, multi­-institutional collaborations including multiple specialisms and a mixture of academic and industrial partners, and demanding security requirements driven by commercial imperatives. This work was undertaken as part of the NanoCMOS project. However, the problems, solutions and experience seem likely to be of wider relevance, both within the CMOS design community and more generally in other disciplines