AB1534-HPR ASSESSMENT OF BACK PAIN IN NURSES WORKING IN HOSPITALS AND OUTPATIENT CLINICS

BackgroundBack pain in a common health problem in nurses affecting 70-78% of them [1,2,3]. The risk of back pain in nurses increases by 35% for each additional working hour per day [2].ObjectivesThe aim of the study was to find out if nurses working in hospital wards suffer more from back pain than their counterparts employed in the outpatient clinics.MethodsAn online survey was conducted with use of an questionnaire containing questions about body mass index (BMI), Revised Oswestry Low Back Pain Disability Scale (ROLBPDS), Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Numeric Rating Scale (NRS) for pain. Questions about seniority and working hours were included.It was uploaded and shared on the Google online survey platform. Participation was anonymous and voluntary. An invitation was mailed to the members of The Lublin Chamber of Nurses and Midwives. A total of 163 nurses (161women and 2 men) aged 21-70 years filled in the questionnaire in February and March 2021.Of the group 82 were nurses from hospitals (NHs) and 81 from outpatient clinics (NOCs).ResultsThe mean BMI of participants was 26.4kg/m2. In NHs the mean BMI was 25kg/m2 and in NOCs 27.8kg/m2.Seventy five percent of HNs looked after lying patients.Ninety nine percent of NHs and 75% of NOCs suffered from back pain. In the NH group 73% reported pain in lumbar, 13% in thoracic and 14% in cervical spine. In the NOC group 15% complained about neck pain and the rest about low back pain. The mean ROLBPDS rating was 15 which means moderate disability. In NOCs it was 13 and in HNs it was 17. The mean back pain NRS was 6. In NOCs it was 3 and in NHs it was 9 (p&lt;0.05). There was a positive correlation between shift length and back pain measured with the NRS. As many as 5 participants had diagnosed seronegative spondyloarthropathy. In those mean BASDAI was 5.6±1 which means active disease.ConclusionNurses working in hospital wards experience significantly more back pain than their counterparts working in outpatient clinics. Long working hours increase the risk for the ailment.References[1]Ibrahim MI, Zubair IU, Yaacob NM, Ahmad MI, Shafei MN. Low Back Pain and Its Associated Factors among Nurses in Public Hospitals of Penang, Malaysia. Int J Environ Res Public Health. 2019 Nov 1;16(21):4254. doi: 10.3390/ijerph16214254. PMID: 31683911; PMCID: PMC6861894.[2]Shieh SH, Sung FC, Su CH, Tsai Y, Hsieh VC. Increased low back pain risk in nurses with high workload for patient care: A questionnaire survey. Taiwan J Obstet Gynecol. 2016 Aug;55(4):525-9. doi: 10.1016/j.tjog.2016.06.013. PMID: 27590376.[3]Wolska D. Wpływ występowania dolegliwości bólowych kręgosłupa na jakość życia pielęgniarek -doniesienia wstępne, Innowacje w Pielęgniarstwie i Naukach o Zdrowiu 2018, 2 (3), 39-54 The impact of back pain on the quality of life of nurses - introductory reports, Innovations in Nursing and Health Sciences 2018, 2 (3), 39-54(in Polish).Disclosure of InterestsNone declared</jats:sec

The influence of poisoning with patulin on activity of acid phosphatase, cathepsin B and D in mice kidneys and livers

Introduction. Patulin is a mycotoxin produced by a variety of moulds, for instance, Aspergillus, Penicillium and Byssochlamys, and found most often in rotten apples. Previous studies showed toxic effects of patulin in the gastro-intestinal tract, impairment of kidney function, as well as neurotoxicity. Objective. The aim of the study was to investigate whether intoxication with patulin affects the activity of acid phosphatase, cathepsin B and D in mice kidneys and livers. Materials and method. Experiments were conducted on 36 female mice. Animals were divided into 6 groups of 6: 1 – control, 2 – received 0.1 LD50 patulin i.p. 28 days, 3 – saline i.p. once, 4 – patulin i.p. 0.1 LD50 once, 5 patulin i.p. 0.2 LD50 once, 6 – patulin i.p. 0.5 LD50 once. 6 hours after patulin or saline administration, animals from groups 3, 4, 5, 6 were sacrificed. Kidneys and livers were obtained. Animals from groups 1 and 2 were sacrificed on day 29. Acid phosphatase activity was measured in the tissue supernatants with colorimetric method. Cathepsin B and D activities were determined with an ELISA-kit. Results. The activities of acid phosphatase, cathepsin B and D in the kidneys and livers of mice exposed to patulin for 28 days was higher than in controls. A proportionate increase in acid phosphatase and cathepsin B activity in the kidneys and livers was observed for a single dose of the xenobiotic. Conclusions. Acute and subacute poisoning with patulin negatively affects the functioning of lysosomes and induces an increase in the activity of lysosomal enzymes in mice livers and kidneys. Activities of acid phosphatase and selected cathepsins in the livers and kidneys are markers of cell damage due to patulin’s toxicity