1. Wochenbericht AL509

Wochenbericht FS Alkor Reise 509, Fahrtabschnitt 15.05 – 20.05.201

Organismen von morgen im Experiment von heute – experimentelle Evolution mit Phytoplankton

Der globale Wandel erfasst zunehmend auch die Weltmeere und ihre Lebewelt. Insbesondere Erwärmung und Ozeanversauerung könnte den Beginn der Nahrungskette beeinträchtigen, die mikroskopisch kleinen Pflanzen des Phytoplanktons. Diese Einzeller tragen zur Hälfte der gesamten Biomasse - Produktion auf unserem Planeten bei. Das allermeiste Wissen über ihre Reaktionen auf die globalen Umweltveränderungen hat die Meeresbiologie aus Kurzzeit-Experimenten. Doch die Organismen von morgen könnten auch ganz anders auf die neuen Bedingungen reagieren, sofern sie sich evolutiv anpassen. Der Vortrag stellt den neuen Ansatz von Evolutionsexperimenten in der Meereskunde vor. Dieser ermöglicht es, die Organismen von morgen heute im Labor zu untersuchen. Wie erwartet können sich einige Arten rechtzeitig anpassen, ihre Anpassungsrate ist dabei schneller als die prognostizierten Umweltänderungen. In einem Ausblick werden die evolutionsbiologischen Konzepte auch auf andere Bereiche in der Meeresbiologie übertragen wie die Fischerei

Spatial variability of climate and past atmospheric circulation patterns from central West Antarctic glaciochemistry

Atmospheric circulation patterns and the spatial variability of atmospheric chemistry and moisture transport in central West Antarctica are investigated using new 40 year long (1954–1994 A.D.) glaciochemical and accumulation rate records developed from four firn cores from this region. The core sites lie on a 200 km traverse from 82° 22′ S, 119° 17′ W to 81° 22′ S, 107° 17′ W. The glaciochemical records represent the major ionic species present in Antarctic snow: Na+, K+, Mg2+, Ca2+, Cl−, NO3−, and SO42−. High spatial variability appears in comparisons of full record averages and poor intersite linear correlation results. Accumulation rates show 50–100% changes over distances of 50–100 km and sea‐salt concentrations drop by 50% between the middle two sites. One likely contributor to the high variability seen at this spatial scale is variability in synoptic‐ and finer‐scale meteorology. Empirical orthogonal function (EOF) analysis shows that 80% or more of the variance in site chemistry can be attributed to two types of air masses: winter season air (50–70% of site variance) with a strong marine signature (heavy loading of sea‐salt species) and summer season air (21% of the variance), marked by marine biogenic non‐sea‐salt SO4 plus NO3. This pattern of winter and summer regimes appears at other West Antarctic sites suggesting it may apply to the entire region. We show that a general picture of the patterns of variability in West Antarctica can best be drawn by using an analysis technique that fully exploits high resolution, multiparameter, multisite data sets

Exact broken-symmetry states and Hartree-Fock solutions for quantum dots at high magnetic fields

Wigner molecules formed at high magnetic fields in circular and elliptic quantum dots are studied by exact diagonalization (ED) and unrestricted Hartree-Fock (UHF) methods with multicenter basis of displaced lowest Landau level wave functions. The broken symmetry states with semi-classical charge density constructed from superpositions of the ED solutions are compared to the UHF results. UHF overlooks the dependence of the few-electron wave function on the actual relative positions of electrons localized in different charge puddles and partially compensates for this neglect by an exaggerated separation of charge islands which are more strongly localized than in the exact broken-symmetry states.Comment: QD2004 proceedings under press in Physica

Impurity effects in few-electron quantum dots: Incipient Wigner molecule regime

Numerically exact path-integral Monte Carlo data are presented for $N\leq 10$ strongly interacting electrons confined in a 2D parabolic quantum dot, including a defect to break rotational symmetry. Low densities are studied, where an incipient Wigner molecule forms. A single impurity is found to cause drastic effects: (1) The standard shell-filling sequence with magic numbers $N=4,6,9$, corresponding to peaks in the addition energy $\Delta(N)$, is destroyed, with a new peak at N=8, (2) spin gaps decrease, (3) for N=8, sub-Hund's rule spin S=0 is induced, and (4) spatial ordering of the electrons becomes rather sensitive to spin. We also comment on the recently observed bunching phenomenon.Comment: 7 pages, 1 table, 4 figures, accepted for publication in Europhysics Letter

Neutralizing monoclonal antibodies define two different functional sites in human interleukin-4

Human interleukin-4 (IL-4) is a small four-helix-bundle protein which is essential for organizing defense reactions against macroparasites, in particular helminths. Human IL-4 also appears to exert a pathophysiological role during various IgE-mediated allergic diseases. Seven different monoclonal antibodies neutralizing the activity of human IL-4 were studied in order to identify functionally important epitopes. A collection of 41 purified IL-4 variants was used to analyse how defined amino acid replacements affect binding affinity for each individual mAb. Specific amino acid positions could be assigned to four different epitopes. mAbs recognizing epitopes on helix A and/or C interfered with IL-4 receptor binding and thus inhibited IL-4 function. However, other mAbs also inhibiting IL-4 function recognized an epitope on helix D of IL-4 and did not inhibit IL-4 binding to the receptor protein. One mAb, recognizing N-terminal and C-terminal residues, partially competed for binding to the receptor. The results of these mAb epitope analyses confirm and extend previous data on the functional consequences of the amino acid replacements which showed that amino acid residues in helices A and C of IL-4 provide a binding site for the cloned IL-4 receptor and that a signalling site in helix D interacts with a further receptor protein

Evaluation of an Electrolyte Analyser for Measurement of Concentrations of Ionized Calcium and Magnesium in Cats

The goal of this study was to evaluate the Nova CRT 8 electrolyte analyser for determination of concentrations of ionized calcium (Cai) and magnesium (Mgi) in cats, todetermine the effects of sample handling and storage and to establish reference ranges. The precision and analytical accuracy of the Nova CRT 8 analyser were good. The concentrations of Cai and Mgi were significantly lower in aerobically handled serum samples than in those handled anaerobically. The concentrations of Cai and Mgi differed significantly among whole blood, plasma and serum. In anaerobically handled serum, the concentration of Cai was stable for 8 h at 22°C, for 5 days at 4°C and for 1 week at −20°C. The concentration of Mgi was stable for 4 h at 22°C but for less than 24 h at 4°C and for less than 1 week at −20°C. In serum from 36 cats, the reference ranges were 1.20-1.35 mmol/L for Cai and 0.47-0.59 mmol/L for Mgi. The Nova CRT 8 electrolyte analyser is suitable for determination of Cai and Mgi concentrations in cats. Anaerobically handled serum samples are recommended and, stored at room temperature, they yield accurate results when analysed within 4

Повышение рационального использования человеческого потенциала

Реферат Выпускная квалификационная работа содержит 131 страницу, 20 рисунков, 39 таблиц, 50 использованных источников, 6 приложений. Ключевые слова: персонал, человеческий потенциал, рациональное использование, адаптация, компетенции, ПАО «ТРК», социальная ответственность компании. . Объектом исследования в работе является: рациональное использование человеческого потенциала компании. . Цель работы - рассмотрение методов повышения рационального использования человеческого потенциала современной компании . В процессе исследования проводились: 1) рассмотрение деятельности компании за 2011 – 2015 гг.; оценка действующей в компании системы управления персоналом и человеческого потенциала компании; 3) внесение предложений по совершенствованию действующей системы управления человеческим потенциалом компании (с расчетом экономической эффективности от внесенных предложений). . В результате исследования: внесены предложения по внедрению в кадровую работу системы периодической сверки компетенций и улучшению работы с кадровым резервом; проведен расчет экономии от внедрения предлагаемых мероприятий . Степень внедрения: запланировано применение внесенных по результатам проведенного исследования предложений в деятельность компании в 2016 г. . Область применения: управление персоналом и человеческим потенциалом в ПАО «ТРК». . Экономическая эффективность/ значимость работы: сумма полученной экономии составит 4 165 тыс. руб. в год. . В будущем планируется: применение внесенных по результатам проведенного исследования предложений в деятельность компании.abstract Final qualifying work includes 131 page, 20 figures, 39 tables, 50 sources used, 6 applications. Keywords: personnel, human potential, rational use, adaptation, competence, PJSC "TRK" social responsibility of the company. . The object of research is in: the rational use of human resources. . Purpose - to examine the methods of enhancing the rational use of the human potential of the modern company. The study carried out: 1) review of the activities of the company for 2011 - 2015 .; assessment of the current in the company personnel management and human resources of the company; 3) make proposals to improve the existing control system of the human potential of the company (with the calculation of the cost-effectiveness of the proposals). . As a result of the study: made suggestions for the implementation of the personnel working in the periodic verification of competency of the system and improvement of work with the personnel reserve; calculated the savings from the implementation of the proposed activities. Degree of implementation: planned application made by the results of the research proposals in the company's activity in 2016. Field of application: personnel management and human potential in PJSC "TRK". . Cost-effectiveness / value of the work: the sum of the savings will amount to 4165 thousand rubles.. in year. . In the future: an application made on the results of the research proposals in the company's activities