Synonymy and stratigraphic ranges of Belemnopsis in the Heterian and Ohauan Stages (Callovian-Tithonian), southwest Auckland, New Zealand.

Belemnopsis stevensi, Belemnopsis maccrawi, and Belemnopsis sp. A (Challinor 1979a) are synonymous; B. stevensi has priority. New belemnite material from Kawhia Harbour and Port Waikato, together with graphical study methods, indicates that many small fragmentary specimens associated with B. stevensi in the lower part of its stratigraphic range are probably the same taxon. B. stevensi has been found only in the Middle and Upper Heterian Stage (Lower Kimmeridgian) at Kawhia and only in the Lower Ohauan Stage (Upper Kimmeridgian) at Port Waikato. This apparently disjunct distribution is attributed to poor exposure in the relevant sections. Belemnopsis kiwiensis n.sp., Belemnopsis cf. sp. B, Belemnopsis sp. B, Belemnopsis sp. D, and Belemnopsis spp. are associated with B. stevensi near the lowest known point in its stratigraphic range. The distribution of stratigraphically useful belemnites within the Heterian and Ohauan Stages is: Conodicoelites spp. (Lower Heterian; correlated with Lower Callovian); Belemnopsis annae (Lower and Middle Heterian; Lower Callovian/Lower Kimmeridgian); Belemnopsis stevensi (Middle Heterian/Lower Ohauan; Kimmeridgian); Belemnopsis keari (Upper Heterian; Kimmeridgian); Belemnopsis trechmanni (Upper Ohauan; Upper Kimmeridgian/Middle Tithonian). The apparently extreme range of Belemnopsis annae remains unexplained. Klondyke Sandstone (new) is recognised as the basal member of Moewaka Formation (Port Waikato area)

Physical characterixation and in vitro biological impact of highly aggregated antibodies separated into size-enriched populations by fluorescence-activated cell sorting

An IgG2 monoclonal antibody (mAb) solution was subjected to stirring, generating high concentrations of nanometer and subvisible particles, which were then successfully size enriched into different size bins by low speed centrifugation or a combination of gravitational sedimentation and Fluorescence-Activated Cell Sorting (FACS). The size-fractionated mAb particles were assessed for their ability to elicit the release of cytokines from a population of donor-derived human peripheral blood mononuclear cells (PBMC) at two phases of the immune response. Fractions enriched in nanometer-sized particles showed a lower response than those enriched in micron-sized particles in this assay. Particles of 5–10 μm in size displayed elevated cytokine release profiles compared to other size ranges. Stir-stressed mAb particles had amorphous morphology, contained protein with partially altered secondary structure, elevated surface hydrophobicity (compared to controls), and trace levels of elemental fluorine. FACS size-enriched the mAb particle samples, yet did not notably alter the overall morphology or composition of particles as measured by Microflow imaging, Transmission Electron Microscopy, and Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy. The utility and limitations of FACS for size separation of mAb particles and potential of in-vitro PBMC studies to rank order the immunogenic potential of various types of mAb particles is discussed

Evolution of the diatoms: insights from fossil, biological and molecular data

Molecular sequence analyses have yielded many important insights into diatom evolution, but there have been few attempts to relate these to the extensive fossil record of diatoms, probably because of unfamiliarity with the data available, which are scattered widely through the geological literature. We review the main features of molecular phylogenies and concentrate on the correspondence between these and the fossil record; we also review the evolution of major morphological, cytological and life cycle characteristics, and possible diatom origins. The first physical remains of diatoms are from the Jurassic, and well-preserved, diverse floras are available from the Lower Cretaceous. Though these are unequivocally identifiable as centric diatoms, none except a possible Stephanopyxis can be unequivocally linked to lineages of extant diatoms, although it is almost certain that members of the Coscinodiscophyceae (radial centrics) and Mediophyceae (polar centrics) were present; some display curious morphological features that hint at an unorthodox cell division mechanism and life cycle. It seems most likely that the earliest diatoms were marine, but recently discovered fossil deposits hint that episodes of terrestrial colonization may have occurred in the Mesozoic, though the main invasion of freshwaters appears to have been delayed until the Cenozoic. By the Upper Cretaceous, many lineages are present that can be convincingly related to extant diatom taxa. Pennate diatoms appear in the late Cretaceous and raphid diatoms in the Palaeocene, though molecular phylogenies imply that raphid diatoms did in fact evolve considerably earlier. Recent evidence shows that diatoms are substantially underclassified at the species level, with many semicryptic or cryptic species to be recognized; however, there is little prospect of being able to discriminate between such taxa in fossil material

Transnationalisierung: transnationale Unternehmen und Geschlecht

Die feministische Organisationsforschung widmet sich der Analyse von Geschlechterungleichheiten in multinationalen Konzernen und der Frage, inwiefern diese durch die Transnationalisierung verändert, abgebaut oder gar verstärkt werden