The pause-initiation limit restricts transcription activation in human cells.

Eukaryotic gene transcription is often controlled at the level of RNA polymerase II (Pol II) pausing in the promoter-proximal region. Pausing Pol II limits the frequency of transcription initiation ('pause-initiation limit'), predicting that the pause duration must be decreased for transcriptional activation. To test this prediction, we conduct a genome-wide kinetic analysis of the heat shock response in human cells. We show that the pause-initiation limit restricts transcriptional activation at most genes. Gene activation generally requires the activity of the P-TEFb kinase CDK9, which decreases the duration of Pol II pausing and thereby enables an increase in the productive initiation frequency. The transcription of enhancer elements is generally not pause limited and can be activated without CDK9 activity. Our results define the kinetics of Pol II transcriptional regulation in human cells at all gene classes during a natural transcription response

Limnogeologúa de Laguna Chungará y cambio climático durante el Holoceno superior en el altiplano chileno septentrional

[Resumen] El estudio sísmico de la Laguna Chungará (69° 30' O, 18° 15'S, 4520 m. s.n.m, Altiplano chileno septentrional) y el análisis sedimentológico de varios sondeos ha permitido reconstruir la evolución de la sedimentación lacustre en el lago durante el Holoceno Superior. Se han identificado dos asociaciones de facies: i) litoral, más somera, compuesta por facies de turbera y in plataforma lacustre, más profunda, compuesta por facies con abundantes fragmentos de Characeae. Estas facies alternan en tres ciclos sedimentarios provocados por fluctuaciones en el nivel del lago. Estas variaciones hidrológicas en la laguna han sido causadas por cambios en el balance hídrico que a su vez reflejan importantes fluctuaciones climáticas durante el Holoceno Superior.[Abstract] We reconstruct the Late Holocene sedimentary history of Laguna Chungará (69° 30' W, 18° 10'5, 4520 m.a.s.l., northern Chilean Altiplano) based on high resolution seismic profiling and sedimentologic analyses of cores. Two sedimentary facies associations have been defined and interpreted: n macrophytedominant littoral, composed of black muds with macrophyte remains and peaty muds, and in Characeae-dominant lacustrine self, composed of gray muds and sands with abundant Characeae remains. The two facies associations define three cycles caused by oscillations in the lake level from shallower (macrophyte) to deeper (Characeae) conditions. Changes in the hydrology of Laguna Chungará reflect variations in the effective moisture (precipitation - evaporation) in the Altiplano during the Late Holocene

Meteorological satellites

An overview is presented of the meteorological satellite programs that have been evolving from 1958 to the present, and plans for the future meteorological and environmental satellite systems that are scheduled to be placed into service in the early 1980's are reviewed. The development of the TIROS family of weather satellites, including TIROS, ESSA, ITOS/NOAA, and the present TIROS-N (the third generation operational system) is summarized. The contribution of the Nimbus and ATS technology satellites to the development of the operational-orbiting and geostationary satellites is discussed. Included are descriptions of both the TIROS-N and the DMSP payloads currently under development to assure a continued and orderly growth of these systems into the 1980's

Topological Reverberations in Flat Space-times

We study the role played by multiply-connectedness in the time evolution of the energy E(t) of a radiating system that lies in static flat space-time manifolds M_4 whose t=const spacelike sections M_3 are compact in at least one spatial direction. The radiation reaction equation of the radiating source is derived for the case where M_3 has any non-trivial flat topology, and an exact solution is obtained. We also show that when the spacelike sections are multiply-connected flat 3-manifolds the energy E(t) exhibits a reverberation pattern with discontinuities in the derivative of E(t) and a set of relative minima and maxima, followed by a growth of E(t). It emerges from this result that the compactness in at least one spatial direction of Minkowski space-time is sufficient to induce this type of topological reverberation, making clear that our radiating system is topologically fragile. An explicit solution of the radiation reaction equation for the case where M_3 = R^2 x S^1 is discussed, and graphs which reveal how the energy varies with the time are presented and analyzed.Comment: 16 pages, 4 figures, REVTEX; Added five references and inserted clarifying details. Version to appear in Int. J. Mod. Phys. A (2000

Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex.

The conserved polymerase-associated factor 1 complex (Paf1C) plays multiple roles in chromatin transcription and genomic regulation. Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II (Pol II) transcription elongation complex (EC). Here we report the reconstitution of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound to a Pol II EC containing the elongation factor TFIIS. Cryo-electron microscopy and crosslinking data reveal that Paf1C is highly mobile and extends over the outer Pol II surface from the Rpb2 to the Rpb3 subunit. The Paf1-Leo1 heterodimer and Cdc73 form opposite ends of Paf1C, whereas Ctr9 bridges between them. Consistent with the structural observations, the initiation factor TFIIF impairs Paf1C binding to Pol II, whereas the elongation factor TFIIS enhances it. We further show that Paf1C is globally required for normal mRNA transcription in yeast. These results provide a three-dimensional framework for further analysis of Paf1C function in transcription through chromatin

Charged pions from Ni on Ni collisions between 1 and 2 AGeV

Charged pions from Ni + Ni reactions at 1.05, 1.45 and 1.93 AGeV are measured with the FOPI detector. The mean $\pi^{\pm}$ multiplicities per mean number of participants increase with beam energy, in accordance with earlier studies of the Ar + KCl and La + La systems. The pion kinetic energy spectra have concave shape and are fitted by the superposition of two Boltzmann distributions with different temperatures. These apparent temperatures depend only weakly on bombarding energy. The pion angular distributions show a forward/backward enhancement at all energies, but not the $\Theta = 90^0$ enhancement which was observed in case of the Au + Au system. These features also determine the rapidity distributions which are therefore in disagreement with the hypothesis of one thermal source. The importance of the Coulomb interaction and of the pion rescattering by spectator matter in producing these phenomena is discussed.Comment: 22 pages, Latex using documentstyle[12pt,a4,epsfig], to appear in Z. Phys.

Pion-Production in Heavy-Ion Collisions at SIS energies

We investigate the production of pions in heavy-ion collisions in the energy range of $1$ - $2$ GeV/A. The dynamics of the nucleus-nucleus collisions is described by a set of coupled transport equations of the Boltzmann-Uehling-Uhlenbeck type for baryons and mesons. Besides the $N(938)$ and the $\Delta(1232)$ we also take into account nucleon resonances up to masses of $1.9 GeV/c^2$ as well as $\pi$-, $\eta$- and $\rho$-mesons. We study in detail the influence of the higher baryonic resonances and the $2\pi$-production channels ($NN\to NN \pi\pi$) on the pion spectra in comparison to $\pi^-$ data from $Ar + KCl$ collisions at $1.8$ GeV/A and $\pi^0$-data for $Au+Au$ at 1.0 GeV/A. We, furthermore, present a detailed comparison of differential pion angular distributions with the BEVALAC data for Ar + KCl at 1.8 GeV/A. The general agreement obtained indicates that the overall reactions dynamics is well described by our novel transport approach.Comment: 31 pages, 18 figures (inlcuded), to appear in Z. Phys.

Neutral Pions and Eta Mesons as Probes of the Hadronic Fireball in Nucleus-Nucleus Collisions around 1A GeV

Chemical and thermal freeze-out of the hadronic fireball formed in symmetric collisions of light, intermediate-mass, and heavy nuclei at beam energies between 0.8A GeV and 2.0A GeV are discussed in terms of an equilibrated, isospin-symmetric ideal hadron gas with grand-canonical baryon-number conservation. For each collision system the baryochemical potential mu_B and the chemical freeze-out temperature T_c are deduced from the inclusive neutral pion and eta yields which are augmented by interpolated data on deuteron production. With increasing beam energy mu_B drops from 800 MeV to 650 MeV, while T_c rises from 55 MeV to 90 MeV. For given beam energy mu_B grows with system size, whereas T_c remains constant. The centrality dependence of the freeze-out parameters is weak as exemplified by the system Au+Au at 0.8A GeV. For the highest beam energies the fraction of nucleons excited to resonance states reaches freeze-out values of nearly 15 %, suggesting resonance densities close to normal nuclear density at maximum compression. In contrast to the particle yields, which convey the status at chemical freeze-out, the shapes of the related transverse-mass spectra do reflect thermal freeze-out. The observed thermal freeze-out temperatures T_th are equal to or slightly lower than T_c, indicative of nearly simultaneous chemical and thermal freeze-out.Comment: 42 pages, 12 figure

Spatio-Temporal Characteristics of Global Warming in the Tibetan Plateau during the Last 50 Years Based on a Generalised Temperature Zone - Elevation Model

Temperature is one of the primary factors influencing the climate and ecosystem, and examining its change and fluctuation could elucidate the formation of novel climate patterns and trends. In this study, we constructed a generalised temperature zone elevation model (GTEM) to assess the trends of climate change and temporal-spatial differences in the Tibetan Plateau (TP) using the annual and monthly mean temperatures from 1961-2010 at 144 meteorological stations in and near the TP. The results showed the following: (1) The TP has undergone robust warming over the study period, and the warming rate was 0.318°C/decade. The warming has accelerated during recent decades, especially in the last 20 years, and the warming has been most significant in the winter months, followed by the spring, autumn and summer seasons. (2) Spatially, the zones that became significantly smaller were the temperature zones of -6°C and -4°C, and these have decreased 499.44 and 454.26 thousand sq km from 1961 to 2010 at average rates of 25.1% and 11.7%, respectively, over every 5-year interval. These quickly shrinking zones were located in the northwestern and central TP. (3) The elevation dependency of climate warming existed in the TP during 1961-2010, but this tendency has gradually been weakening due to more rapid warming at lower elevations than in the middle and upper elevations of the TP during 1991-2010. The higher regions and some low altitude valleys of the TP were the most significantly warming regions under the same categorizing criteria. Experimental evidence shows that the GTEM is an effective method to analyse climate changes in high altitude mountainous regions

Influence of the in-medium pion dispersion relation in heavy ion collisions

We investigate the influence of medium corrections to the pion dispersion relation on the pion dynamics in intermediate energy heavy ion collisions. To do so a pion potential is extracted from the in-medium dispersion relation and used in QMD calculations and thus we take care of both, real and imaginary part of the pion optical potential. The potentials are determined from different sources, i.e. from the $\Delta$--hole model and from phenomenological approaches. Depending on the strength of the potential a reduction of the anti-correlation of pion and nucleon flow in non-central collisions is observed as well as an enhancement of the high energetic yield in transverse pion spectra. A comparison to experiments, in particular to $p_t$-spectra for the reaction Ca+Ca at 1 GeV/nucleon and the pion in-plane flow in Ne+Pb collisions at 800 MeV/nucleon, generally favours a weak potential.Comment: 25 pages, using REVTeX, 6 postscript figures; replaced by published versio