Exocytosis from permeabilized bovine adrenal chromaffin cells is differently modulated by guanosine 5'-[gamma-thio]triphosphate and guanosine 5'-[beta gamma-imido]triphosphate

1. In bovine adrenal chromaffin cells made permeable either to molecules less than or equal to 3 kDa with alphatoxin or to proteins less than or equal to 150 kDa with streptolysin O, the GTP analogues guanosine 5'-[beta gamma-imido]triphosphate (p[NH]ppG) and guanosine 5'-[gamma-thio]triphosphate (GTP[S]) differently modulated Ca(2+)-stimulated exocytosis. 2. In alphatoxin-permeabilized cells, p[NH]ppG up to 20 microM activated Ca(2+)-stimulated exocytosis. Higher concentrations had little or no effect. At a free Ca2+ concentration of 5 microM, 7 microM-p[NH]ppG stimulated exocytosis 6-fold. Increasing the free Ca2+ concentration reduced the effect of p[NH]ppG. Pretreatment of the cells with pertussis toxin prevented the activation of the Ca(2+)-stimulated exocytosis by p[NH]ppG. 3. In streptolysin O-permeabilized cells, p[NH]ppG did not activate, but rather inhibited Ca(2+)-dependent catecholamine release under all conditions studied. In the soluble cytoplasmic material that escaped during permeabilization with streptolysin O, different G-protein alpha-subunits were detected using an appropriate antibody. Around 15% of the cellular alpha-subunits were detected in the supernatant of permeabilized control cells. p[NH]ppG or GTP[S] stimulated the release of alpha-subunits 2-fold, causing a loss of about 30% of the cellular G-protein alpha-subunits under these conditions. Two of the alpha-subunits in the supernatant belonged to the G(o) type, as revealed by an antibody specific for G(o) alpha. 4. GTP[S], when present alone during stimulation with Ca2+, activated exocytosis in a similar manner to p[NH]ppG. Upon prolonged incubation, GTP[S], in contrast to p[NH]ppG, inhibited Ca(2+)-induced exocytosis from cells permeabilized by either of the pore-forming toxins. This effect was resistant to pertussin toxin. 5. The p[NH]ppG-induced activation of Ca(2+)-stimulated release from alphatoxin-permeabilized chromaffin cells may be attributed to one of the heterotrimeric G-proteins lost during permeabilization with streptolysin O. The inhibitory effect of GTP[S] on exocytosis is apparently not mediated by G-protein alpha-subunits, but by another GTP-dependent process still occurring after permeabilization with streptolysin O

Efimov physics in heteronuclear four-body systems

We study three- and four-body Efimov physics in a heteronuclear atomic system with three identical heavy bosonic atoms and one light atom. We show that exchange of the light atom between the heavy atoms leads to both three- and four-body features in the low-energy inelastic rate constants that trace to the Efimov effect. Further, the effective interaction generated by this exchange can provide an additional mechanism for control in ultracold experiments. Finally, we find that there is no true four-body Efimov effect - that is, no infinite number of four-body states in the absence of two- and three-body bound states - resolving a decades-long controversy.Comment: 5 pages, 4 figure

Double Quantum Dots in Carbon Nanotubes

We study the two-electron eigenspectrum of a carbon-nanotube double quantum dot with spin-orbit coupling. Exact calculation are combined with a simple model to provide an intuitive and accurate description of single-particle and interaction effects. For symmetric dots and weak magnetic fields, the two-electron ground state is antisymmetric in the spin-valley degree of freedom and is not a pure spin-singlet state. When double occupation of one dot is favored by increasing the detuning between the dots, the Coulomb interaction causes strong correlation effects realized by higher orbital-level mixing. Changes in the double-dot configuration affect the relative strength of the electron-electron interactions and can lead to different ground state transitions. In particular, they can favor a ferromagnetic ground state both in spin and valley degrees of freedom. The strong suppression of the energy gap can cause the disappearance of the Pauli blockade in transport experiments and thereby can also limit the stability of spin-qubits in quantum information proposals. Our analysis is generalized to an array of coupled dots which is expected to exhibit rich many-body behavior.Comment: 14 pages, 11 pages and 1 table. Typos in text and Figs.4 and 6 correcte

Amylase release from streptolysin O-permeabilized pancreatic acinar cells. Effects of Ca2+, guanosine 5'-[gamma-thio]triphosphate, cyclic AMP, tetanus toxin and botulinum A toxin

The molecular requirements for amylase release and the intracellular effects of botulinum A toxin and tetanus toxin on amylase release were investigated using rat pancreatic acinar cells permeabilized with streptolysin O. Micromolar concentrations of free Ca2+ evoked amylase release from these cells. Maximal release was observed in the presence of 30 microM free Ca2+. Ca(2+)-stimulated, but not basal, amylase release was enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[S]) (3-4 fold) or cyclic AMP (1.5-2 fold). Neither the two-chain forms of botulinum A toxin and tetanus toxin, under reducing conditions, nor the light chains of tetanus toxin, inhibited amylase release triggered by Ca2+, or combinations of Ca2+ + GTP[S] or Ca2+ + cAMP. The lack of inhibition was not due to inactivation of botulinum A toxin or tetanus toxin by pancreatic acinar cell proteolytic enzymes, as toxins previously incubated with permeabilized pancreatic acinar cells inhibited Ca(2+)-stimulated [3H]noradrenaline release from streptolysin O-permeabilized adrenal chromaffin cells. These data imply that clostridial neurotoxins inhibit a Ca(2+)-dependent mechanism which promotes exocytosis in neural and endocrine cells, but not in exocrine cells

Exploring the functional domain and the target of the tetanus toxin light chain in neurohypophysial terminals

The tetanus toxin light chain blocks calcium induced vasopressin release from neurohypophysial nerve terminals. Here we show that histidine residue 233 within the putative zinc binding motif of the tetanus toxin light chain is essential for the inhibition of exocytosis, in the rat. The zinc chelating agent dipicolinic acid as well as captopril, an inhibitor of zinc-dependent peptidases, counteract the effect of the neurotoxin. Synthetic peptides, the sequences of which correspond to motifs present in the cytoplasmic domain of the synaptic vesicle membrane protein synaptobrevin 1 and 2, prevent the effect of the tetanus toxin light chain. Our results indicate that zinc bound to the zinc binding motif constitutes the active site of the tetanus toxin light chain. Moreover they suggest that cleavage of synaptobrevin by the neurotoxin causes the inhibition of exocytotic release of vasopressin from secretory granules

BEC-BCS Crossover of a Trapped Two-Component Fermi Gas with Unequal Masses

We determine the energetically lowest lying states in the BEC-BCS crossover regime of s-wave interacting two-component Fermi gases under harmonic confinement by solving the many-body Schrodinger equation using two distinct approaches. Essentially exact basis set expansion techniques are applied to determine the energy spectrum of systems with N=4 fermions. Fixed-node diffusion Monte Carlo methods are applied to systems with up to N=20 fermions, and a discussion of different guiding functions used in the Monte Carlo approach to impose the proper symmetry of the fermionic system is presented. The energies are calculated as a function of the s-wave scattering length a_s for N=2-20 fermions and different mass ratios \kappa of the two species. On the BEC and BCS sides, our energies agree with analytically-determined first-order correction terms. We extract the scattering length and the effective range of the dimer-dimer system up to \kappa = 20. Our energies for the strongly-interacting trapped system in the unitarity regime show no shell structure, and are well described by a simple expression, whose functional form can be derived using the local density approximation, with one or two parameters. The universal parameter \xi for the trapped system for various \kappa is determined, and comparisons with results for the homogeneous system are presented.Comment: 11 pages, 6 figures, extended versio

Efimov physics beyond three particles

Efimov physics originally refers to a system of three particles. Here we review recent theoretical progress seeking for manifestations of Efimov physics in systems composed of more than three particles. Clusters of more than three bosons are tied to each Efimov trimer, but no independent Efimov physics exists there beyond three bosons. The case of a few heavy fermions interacting with a lighter atom is also considered, where the mass ratio of the constituent particles plays a significant role. Following Efimov's study of the (2+1) system, the (3+1) system was shown to have its own critical mass ratio to become Efimovian. We show that the (4+1) system becomes Efimovian at a mass ratio which is smaller than its sub-systems thresholds, giving a pure five-body Efimov effect. The (5+1) and (6+1) systems are also discussed, and we show the absence of 6- and 7-body Efimov physics there

Far-Ultraviolet Color Gradients in Early-Type Galaxies

We discuss far-UV (1500 A) surface photometry and FUV-B color profiles for 8 E/S0 galaxies from images taken with the Ultraviolet Imaging Telescope, primarily during the Astro-2 mission. In three cases, the FUV radial profiles are more consistent with an exponential than a de Vaucouleurs function, but there is no other evidence for the presence of a disk or of young, massive stars. In all cases except M32 the FUV-B color becomes redder at larger radii. There is a wide range of internal radial FUV-B color gradients. However, we find no correlation between the FUV-B color gradients and internal metallicity gradients based on Mg absorption features. We conclude that metallicity is not the sole parameter controlling the "UV upturn component" in old populations.Comment: 11 pages; tar.gz file includes LaTeX text file, 3 PostScript figures. Paper to be published in ApJ Letter