Comparison of TCGA and GENIE genomic datasets for the detection of clinically actionable alterations in breast cancer.

Whole exome sequencing (WES), targeted gene panel sequencing and single nucleotide polymorphism (SNP) arrays are increasingly used for the identification of actionable alterations that are critical to cancer care. Here, we compared The Cancer Genome Atlas (TCGA) and the Genomics Evidence Neoplasia Information Exchange (GENIE) breast cancer genomic datasets (array and next generation sequencing (NGS) data) in detecting genomic alterations in clinically relevant genes. We performed an in silico analysis to determine the concordance in the frequencies of actionable mutations and copy number alterations/aberrations (CNAs) in the two most common breast cancer histologies, invasive lobular and invasive ductal carcinoma. We found that targeted sequencing identified a larger number of mutational hotspots and clinically significant amplifications that would have been missed by WES and SNP arrays in many actionable genes such as PIK3CA, EGFR, AKT3, FGFR1, ERBB2, ERBB3 and ESR1. The striking differences between the number of mutational hotspots and CNAs generated from these platforms highlight a number of factors that should be considered in the interpretation of array and NGS-based genomic data for precision medicine. Targeted panel sequencing was preferable to WES to define the full spectrum of somatic mutations present in a tumor

The role of social cognition in decision making

Successful decision making in a social setting depends on our ability to understand the intentions, emotions and beliefs of others. The mirror system allows us to understand other people's motor actions and action intentions. ‘Empathy’ allows us to understand and share emotions and sensations with others. ‘Theory of mind’ allows us to understand more abstract concepts such as beliefs or wishes in others. In all these cases, evidence has accumulated that we use the specific neural networks engaged in processing mental states in ourselves to understand the same mental states in others. However, the magnitude of the brain activity in these shared networks is modulated by contextual appraisal of the situation or the other person. An important feature of decision making in a social setting concerns the interaction of reason and emotion. We consider four domains where such interactions occur: our sense of fairness, altruistic punishment, trust and framing effects. In these cases, social motivations and emotions compete with each other, while higher-level control processes modulate the interactions of these low-level biases

Penerapan Model Pembelajaran Learning Cycle 5e untuk Meningkatkan Keterampilan Proses Sains Siswa

Penelitian bertujuan untuk mendapatkan gambaran mengenai peningkatan Keterampilan Proses Sains siswa setelah diterapkan model pembelajaran Learning Cycle 5E. Metode penelitian yang digunakan dalam penelitian ini adalah metode eksperimen. Desain penelitian yang digunakan adalah poor experimental design, dengan rancangan penelitian one group pretest-posttest design. Penelitian ini dilaksanakan di SMA Negeri 7 Tangerang Selatan. Populasi penelitian ini adalah siswa kelas XI IPA tahun ajaran 2016/2017. Sampel yang digunakan adalah siswa kelas XI IPA 1, sampel diambil dari populasi menggunakan teknik purposive sampling. Instrumen yang digunakan dalam penelitian ini antara lain instrumen tes yang berupa soal pilihan ganda dan lembar observasi. Berdasarkan hasil analisis yang dilakukan diperoleh besar peningkatan keterampilan proses sains setelah diterapkan model pembelajaran Learning Cycle 5E dengan N-gain sebesar 0,5696 yang termasuk dalam kategori peningkatan sedang

Optimal combination of signals from co-located gravitational wave interferometers for use in searches for a stochastic background

This article derives an optimal (i.e., unbiased, minimum variance) estimator for the pseudo-detector strain for a pair of co-located gravitational wave interferometers (such as the pair of LIGO interferometers at its Hanford Observatory), allowing for possible instrumental correlations between the two detectors. The technique is robust and does not involve any assumptions or approximations regarding the relative strength of gravitational wave signals in the detector pair with respect to other sources of correlated instrumental or environmental noise. An expression is given for the effective power spectral density of the combined noise in the pseudo-detector. This can then be introduced into the standard optimal Wiener filter used to cross-correlate detector data streams in order to obtain an optimal estimate of the stochastic gravitational wave background. In addition, a dual to the optimal estimate of strain is derived. This dual is constructed to contain no gravitational wave signature and can thus be used as on "off-source" measurement to test algorithms used in the "on-source" observation.Comment: 14 pages, 4 figures, submitted to Physical Review D Resubmitted after editing paper in response to referee comments. Removed appendices A, B and edited text accordingly. Improved legibility of figures. Corrected several references. Corrected reference to science run number (S1 vs. S2) in text and figure caption

Randomisation of Pulse Phases for Unambiguous and Robust Quantum Sensing

We develop theoretically and demonstrate experimentally a universal dynamical decoupling method for robust quantum sensing with unambiguous signal identification. Our method uses randomisation of control pulses to suppress simultaneously two types of errors in the measured spectra that would otherwise lead to false signal identification. These are spurious responses due to finite-width $\pi$ pulses, as well as signal distortion caused by $\pi$ pulse imperfections. For the cases of nanoscale nuclear spin sensing and AC magnetometry, we benchmark the performance of the protocol with a single nitrogen vacancy centre in diamond against widely used non-randomised pulse sequences. Our method is general and can be combined with existing multipulse quantum sensing sequences to enhance their performance

Estimation Of Net Primary Production (Npp) Using Remote Sensing Approach And Plant Physiological Modeling(pendugaan Net Primary Production (Npp) Menggunakan Pendekatan Penginderaan Jauh dan Modeling Fisiologis Tanaman)

Information Net Primary Production (NPP) of tropical forests is important for the development of realistic global carbon budgets and for projecting how these ecosystems will be affected by climate changes. This research utilized remotely sensed data and micrometeorological measurement to provide information on vegetation condition. The objective of this research is to estimate spatial NPP using remote sensing approach and plant physiological/micrometeorological modeling. The estimation of NPP is conducted using modeling approach, which is based on relationship between radiation use efficiency, photosyntetically active radiation and fraction of absorbed photosynthetically active radiation by the plants's canopy. Trend of NDVI derived using micrometeorological measurement showed an increase from 2001 to 2002, and then decrease from 2002 to 2004. Average different values (delta) between both methods used to derive NDVI is relatively constant around 0.33 with a high correlation of r2 = 0.98. Using remotely sensed data, the highest NPP values estimated is in year 2003 with value range between 2000 – 2500 (gC m-2 yr-1), less than 2% of the whole forest area. In 2003, 75% area has NPP between 1500 – 2000 (gC m-2 yr-1), meanwhile for 2002 and 2004 it is only 21% and 50 %, respectively. NPP values estimated using micrometeorological measurement show the increasing of NPP values from 2002 to 2003, and then decrease from 2003 to 2004. There is strong correlation between NPP values derived from the two methods with r2 = 0.98

Characterization of Physical and Motor Disability at the Technical University of Manabí

The work aims to determine the characterization of physical and motor disability and its relation to resilience in the students of the Technical University of Manabí. The paper shows an analysis of physical motor disability in students and its impact on the level of resilience. The resilience analysis was addressed terms physical and mental state of the people, as well as the process of adapting to the problems and adversities of life. The results were shown in tables and graph on the analysis of the degree of physical-motor disability of the students as well as the application of the SV-RES test prepared by the researchers Saavedra & Villalta, 2008. Based on the evaluation of 12 dimensions, which they allow to verify the situation according to three scales: high, average and low. Finally, the conclusions were offered where the objective stated in the investigation was verified