Biochar successfully replaces activated charcoal for in vitro culture of two white poplar clones reducing ethylene concentration

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Exposure to depleted uranium does not alter the co-expression of HER-2/neu and p53 in breast cancer patients

<p>Abstract</p> <p>Background</p> <p>Amongst the extensive literature on immunohistochemical profile of breast cancer, very little is found on populations exposed to a potential risk factor such as depleted uranium. This study looked at the immunohistochemical expression of HER-2/neu (c-erbB2) and p53 in different histological types of breast cancer found in the middle Euphrates region of Iraq, where the population has been exposed to high levels of depleted uranium.</p> <p>Findings</p> <p>The present investigation was performed over a period starting from September 2008 to April 2009. Formalin-fixed, paraffin-embedded blocks from 70 patients with breast cancer (62 ductal and 8 lobular carcinoma) were included in this study. A group of 25 patients with fibroadenoma was included as a comparative group, and 20 samples of normal breast tissue sections were used as controls. Labeled streptavidin-biotin (LSAB+) complex method was employed for immunohistochemical detection of HER-2/neu and p53.</p> <p>The detection rate of HER-2/neu and p53 immunohistochemical expression were 47.14% and 35.71% respectively in malignant tumors; expression was negative in the comparative and control groups (p < 0.05).</p> <p>HER-2/neu immunostaining was significantly associated with histological type, tumor size, nodal involvement, and recurrence of breast carcinoma (<it>p </it>< 0.05), p53 immunostaining was significantly associated with tumor size, nodal involvement and recurrence of breast cancer (<it>p </it>< 0.05). There was greater immunoexpression of HER-2/neu in breast cancer in this population, compared with findings in other populations.</p> <p>Both biomarkers were positively correlated with each other. Furthermore, all the cases that co-expressed both HER-2/neu and p53 showed the most unfavorable biopathological profile.</p> <p>Conclusion</p> <p>P53 and HER-2/neu over-expression play an important role in pathogenesis of breast carcinoma. The findings indicate that in regions exposed to high levels of depleted uranium, although p53 and HER-2/neu overexpression are both high, correlation of their expression with age, grade, tumor size, recurrence and lymph node involvement is similar to studies that have been conducted on populations not exposed to depleted uranium. HER-2/neu expression in breast cancer was higher in this population, compared with results on non-exposed populations.</p

Tissue culture of ornamental cacti

Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family

Possible Future Risks of Pollution Consequent to the Expansion of Oil and Gas Operations in Qatar

The air, water, and lands of the Arabian Gulf countries are exposed to contamination involving organic and inorganic components resulting from industrial energy sector activities. In Qatar, marine life and air are the primary elements of the ecosystem that pollution has negatively affected since the discovery and exportation of oil and gas. For example, the mean concentration of PM2.5 reached 105 &amp;micro;g/m3 in 2016. This poor air quality has been attributed to several factors: dust storms, vehicle emissions, and industrial emissions. Marine life around the peninsula of Qatar has been threatened by many factors, including discharge of desalinated seawater, oil and gas activities, and the impact of climate change. Studies conducted after multiple major events showed that levels of various types of pollutants were at acceptable levels. Some areas in the Arabian Gulf, such as the coasts of Saudi Arabia and Bahrain, are still considered chronically polluted and need continual monitoring in the long term. This review discusses the pollution status on the Qatari coastlines and the reasons behind the persistence of current levels of pollution in Arabian Gulf water. The role of microorganisms (bacteria, algae, and fungi) in a biological approach for environmental manipulation of pollution problems is discussed. The agricultural lands in Qatar are possible sites of pollution due to the potential expansion of the energy, industry, and construction sectors in the future. Currently, industrial wastewater is pumped deep into the ground, and seawater is intruding into the main-land, which is causing significant contamination of soils used for the cultivation of various crops. Possible measures are reported, and practical solutions to future pollution risks are discussed.</jats:p

Cyanoremediation of Polluted Seawater in the Arabian Gulf: Risks and Benefits to Human Health

Cyanobacteria play a crucial role in marine ecosystems as primary producers of food and oxygen for various organisms while helping remove waste and toxic substances from the environment. They are essential to the carbon cycle and help regulate the climate. These marine autotrophs also aid in the absorption of essential elements and support diverse life forms. They help degrade organic compounds, including petroleum hydrocarbons as well as heavy metals. Fluctuations in cyanobacteria populations can indicate ecosystem health, influencing both human well-being and wildlife. Their significance also extends to potential technological advancements, thus providing valuable resources for fields such as pharmacology, medicine, health care, biofuels, cosmetics, and bioremediation. However, some species produce toxins that pose risks to human health and marine organisms. Consequently, cyanobacteria are a major focus of research aimed at preserving and improving marine ecosystems&mdash;especially given the environmental damage caused by past and potential future conflicts. This review highlights their roles in cyanoremediation and other industrial and biotechnological applications with a particular focus on the Arabian Gulf region