Biological control of the bronze bug, Thaumastocoris peregrinus, in eucalyptus plantations in Brazil.

Edição dos abstracts do 24º IUFRO World Congress, 2014, Salt Lake City. Sustaining forests, sustaining people: the role of research

Brachymeria pandora (Crawford) (Hymenoptera: Chalcididae) as a New Parasitoid of Thyrinteina leucocerae (Rindge) (Lepidoptera: Geometridae) in Brazil

This is the first report of Brachymeria pandora (Crawford) (Hymenoptera: Chalcididae)-parasitizing pupae of the eucalyptus defoliator Thyrinteina leucocerae (Rindge) (Lepidoptera: Geometridae) in Brazil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Espírito Santo (FAPES)Univ Estadual Paulista, Dept Portecao Plantas, Escola Ciencias Agr, Botucatu, SP, BrazilUniversidade Federal do Espírito Santo (UFES), Dept Ciencias Biol, Ctr Ciencias Humanas & Nat, Vitoria, ES, BrazilUniv Estadual Paulista, Dept Portecao Plantas, Escola Ciencias Agr, Botucatu, SP, Brazi

Computational identification of a transiently open L1/S3 pocket for reactivation of mutant p53

The tumour suppressor p53 is the most frequently mutated gene in human cancer. Reactivation of mutant p53 by small molecules is an exciting potential cancer therapy. Although several compounds restore wild-type function to mutant p53, their binding sites and mechanisms of action are elusive. Here computational methods identify a transiently open binding pocket between loop L1 and sheet S3 of the p53 core domain. Mutation of residue Cys124, located at the centre of the pocket, abolishes p53 reactivation of mutant R175H by PRIMA-1, a known reactivation compound. Ensemble-based virtual screening against this newly revealed pocket selects stictic acid as a potential p53 reactivation compound. In human osteosarcoma cells, stictic acid exhibits dose-dependent reactivation of p21 expression for mutant R175H more strongly than does PRIMA-1. These results indicate the L1/S3 pocket as a target for pharmaceutical reactivation of p53 mutants