Draft Genome Sequence of Photorhabdus temperata Strain Meg1, an Entomopathogenic Bacterium Isolated from Heterorhabditis megidis Nematodes

Photorhabdus temperata strain Meg1 is an entomopathogenic bacterium that forms a symbiotic association with Heterorhabditis nematodes. We report here a 4.9-Mbp draft genome sequence for P. temperata strain Meg1, with a G+C content of 43.18% and containing 4,340 candidate protein-coding genes

Temperature and Field Dependence of the Energy Gap of MgB2/Pb planar junction

We have constructed MgB2/Pb planar junctions for both temperature and field dependence studies. Our results show that the small gap is a true bulk property of MgB2 superconductor, not due to surface effects. The temperature dependence of the energy gap manifests a nearly BCS-like behavior. Analysis of the effect of magnetic field on junctions suggests that the energy gap of MgB2 depends non-linearly on the magnetic field. Moreover, MgB2 has an upper critical field of 15 T, in agreement with some reported Hc2 from transport measurements.Comment: 5 pages, 5 figures. Submitted to Phys. Rev.

Draft Genome Sequence of Photorhabdus luminescens Strain BA1, an Entomopathogenic Bacterium Isolated from Nematodes Found in Egypt

Photorhabdus luminescens strain BA1 is an entomopathogenic bacterium that forms a symbiotic association with Heterorhabditis nematodes. We report here a 5.0-Mbp draft genome sequence for P. luminscens strain BA1, with a G+C content of 42.46% and 4,250 candidate protein-coding genes

Scanning Tunneling Spectroscopy in MgB 2

We present extensive Scanning Tunneling Spectroscopy (STM/S) measurements at low temperatures in the multiband superconductor MgB$_2$. We find a similar behavior in single crystalline samples and in single grains, which clearly shows the partial superconducting density of states of both the $\pi$ and $\sigma$ bands of this material. The superconducting gaps corresponding to both bands are not single valued. Instead, we find a distribution of superconducting gaps centered around 1.9mV and 7.5mV, corresponding respectively to each set of bands. Interband scattering effects, leading to a single gap structure at 4mV and a smaller critical temperature can be observed in some locations on the surface. S-S junctions formed by pieces of MgB$_2$ attached to the tip clearly show the subharmonic gap structure associated with this type of junctions. We discuss future developments and possible new effects associated with the multiband nature of superconductivity in this compound.Comment: 11 pages, 6 figures, submitted to Physica

Effects of diet and exercise on weight-related outcomes for breast cancer survivors and their adult daughters: an analysis of the DAMES trial

Purpose Few trials have aimed to promote diet and exercise behaviors in both cancer survivors and their family members and examine their associations with weight-related outcomes. We conducted a secondary analysis to examine associations between change in diet and exercise behaviors and weight-related outcomes for overweight breast cancer survivors and their overweight adult daughters in the Daughters And MothErS Against Breast Cancer (DAMES) randomized trial. Methods The DAMES trial assessed the impact of two iteratively tailored, mailed print diet and exercise interventions against standard brochures over a 12-month period. This analysis examined change in diet and exercise behaviors and weight-related variables from baseline to post-intervention for the 50 breast cancer survivors and their adult daughters randomized to the intervention arms. To reduce the potential for type II error in this pilot, p values <0.10 were considered statistically significant. Results For mothers, change in diet quality was uniquely related to change in BMI (β = −0.12, p = 0.082), weight (β = −0.12, p = 0.060), and waist circumference (β = −0.38, p = 0.001), whereas change in caloric intake was related to waist circumference (β = 0.21, p = 0.002). For daughters, change in caloric intake was related to change in waist circumference (β = 0.12, p = 0.055). However, change in diet quality was not associated with weight-related outcomes in daughters. Additionally, change in exercise was not associated with weight-related outcomes in mothers or daughters. Conclusions Findings support mail-based and other tailored interventions for weight loss in this population, with an emphasis on diet quality for breast cancer survivors and caloric intake for their adult daughters

Interdisciplinary assessment of sea-level rise and climate change impacts on the lower Nile delta, Egypt

Copyright © 2015 Elsevier. NOTICE: this is the author’s version of a work that was accepted for publication in Science of the Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Science of the Total Environment Vol. 503-504 (2015), DOI: 10.1016/j.scitotenv.2014.06.111CLImate-induced changes on WAter and SECurity (CLIWASEC) was a cluster of three complementary EC-FP7 projects assessing climate-change impacts throughout the Mediterranean on: hydrological cycles (CLIMB - CLimate-Induced changes on the hydrology of Mediterranean Basins); water security (WASSERMed - Water Availability and Security in Southern EuRope and the Mediterranean) and human security connected with possible hydro-climatic conflicts (CLICO - CLImate change hydro-COnflicts and human security). The Nile delta case study was common between the projects. CLIWASEC created an integrated forum for modelling and monitoring to understand potential impacts across sectors. This paper summarises key results from an integrated assessment of potential challenges to water-related security issues, focusing on expected sea-level rise impacts by the middle of the century. We use this common focus to illustrate the added value of project clustering. CLIWASEC pursued multidisciplinary research by adopting a single research objective: sea-level rise related water security threats, resulting in a more holistic view of problems and potential solutions. In fragmenting research, policy-makers can fail to understand how multiple issues can materialize from one driver. By combining efforts, an integrated assessment of water security threats in the lower Nile is formulated, offering policy-makers a clearer picture of inter-related issues to society and environment. The main issues identified by each project (land subsidence, saline intrusion - CLIMB; water supply overexploitation, land loss - WASSERMed; employment and housing security - CLICO), are in fact related. Water overexploitation is exacerbating land subsidence and saline intrusion, impacting on employment and placing additional pressure on remaining agricultural land and the underdeveloped housing market. All these have wider implications for regional development. This richer understanding could be critical in making better policy decisions when attempting to mitigate climate and social change impacts. The CLIWASEC clustering offers an encouraging path for the new European Commission Horizon 2020 programme to follow.European Commission Seventh Framework Program CLICOEuropean Commission Seventh Framework Program CLIMBEuropean Commission Seventh Framework Program WASSERMe

Evolutionary relationships between Rhynchosporium lolii sp. nov. and other Rhynchosporium species on grass.

Copyright: 2013 King et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedThe fungal genus Rhynchosporium (causative agent of leaf blotch) contains several host-specialised species, including R. commune (colonising barley and brome-grass), R. agropyri (couch-grass), R. secalis (rye and triticale) and the more distantly related R. orthosporum (cocksfoot). This study used molecular fingerprinting, multilocus DNA sequence data, conidial morphology, host range tests and scanning electron microscopy to investigate the relationship between Rhynchosporium species on ryegrasses, both economically important forage grasses and common wild grasses in many cereal growing areas, and other plant species. Two different types of Rhynchosporium were found on ryegrasses in the UK. Firstly, there were isolates of R. commune that were pathogenic to both barley and Italian ryegrass. Secondly, there were isolates of a new species, here named R. lolii, that were pathogenic only to ryegrass species. R. lolii was most closely related to R. orthosporum, but exhibited clear molecular, morphological and host range differences. The species was estimated to have diverged from R. orthosporum ca. 5735 years before the present. The colonisation strategy of all of the different Rhynchosporium species involved extensive hyphal growth in the sub-cuticular regions of the leaves. Finally, new species-specific PCR diagnostic tests were developed that could distinguish between these five closely related Rhynchosporium species.Peer reviewedFinal Published versio

Cell arrest and cell death in mammalian preimplantation development

The causes, modes, biological role and prospective significance of cell death in preimplantation development in humans and other mammals are still poorly understood. Early bovine embryos represent a very attractive experimental model for the investigation of this fundamental and important issue. To obtain reference data on the temporal and spatial occurrence of cell death in early bovine embryogenesis, three-dimensionally preserved embryos of different ages and stages of development up to hatched blastocysts were examined in toto by confocal laser scanning microscopy. In parallel, transcript abundance profiles for selected apoptosis-related genes were analyzed by real-time reverse transcriptase-polymerase chain reaction. Our study documents that in vitro as well as in vivo, the first four cleavage cycles are prone to a high failure rate including different types of permanent cell cycle arrest and subsequent non-apoptotic blastomere death. In vitro produced and in vivo derived blastocysts showed a significant incidence of cell death in the inner cell mass (ICM), but only in part with morphological features of apoptosis. Importantly, transcripts for CASP3, CASP9, CASP8 and FAS/FASLG were not detectable or found at very low abundances. In vitro and in vivo, errors and failures of the first and the next three cleavage divisions frequently cause immediate embryo death or lead to aberrant subsequent development, and are the main source of developmental heterogeneity. A substantial occurrence of cell death in the ICM even in fast developing blastocysts strongly suggests a regular developmentally controlled elimination of cells, while the nature and mechanisms of ICM cell death are unclear. Morphological findings as well as transcript levels measured for important apoptosis-related genes are in conflict with the view that classical caspase-mediated apoptosis is the major cause of cell death in early bovine development

Bond-graph Input-State-Output Port-Hamiltonian formulation of memristive networks for emulation of Josephson junction circuits

A bond graph Input-State-Output Port-Hamiltonian formulation of memristive networks for Josephson junction circuits in state space is presented. The methodology has applications to the modeling of SQUIDs and other non-linear transducers and enables the formulation of input-output models of complex components embedded in non-linear networks

Economic factors influencing zoonotic disease dynamics: demand for poultry meat and seasonal transmission of avian influenza in Vietnam

While climate is often presented as a key factor influencing the seasonality of diseases, the importance of anthropogenic factors is less commonly evaluated. Using a combination of methods-wavelet analysis, economic analysis, statistical and disease transmission modelling-we aimed to explore the influence of climatic and economic factors on the seasonality of H5N1 Highly Pathogenic Avian Influenza in the domestic poultry population of Vietnam. We found that while climatic variables are associated with seasonal variation in the incidence of avian influenza outbreaks in the North of the country, this is not the case in the Centre and the South. In contrast, temporal patterns of H5N1 incidence are similar across these 3 regions: periods of high H5N1 incidence coincide with Lunar New Year festival, occurring in January-February, in the 3 climatic regions for 5 out of the 8 study years. Yet, daily poultry meat consumption drastically increases during Lunar New Year festival throughout the country. To meet this rise in demand, poultry production and trade are expected to peak around the festival period, promoting viral spread, which we demonstrated using a stochastic disease transmission model. This study illustrates the way in which economic factors may influence the dynamics of livestock pathogens