Cama-de-frango em mono e policultivo de fáfia com cravo-de-defunto e manjericão Poultry manure in mono and intercrop of Brazilian ginseng with marigold and basil

O experimento foi conduzido em campo da Universidade Federal da Grande Dourados (UFGD), em Dourados-MS, de março de 2005 a setembro de 2006, em Latossolo Vermelho distroférrico. O objetivo foi avaliar a produção da Pfaffia glomerata (Spreng) Pedersen (fáfia) em monocultivo e os policultivos com Tagetes erecta L. (cravo-de-defunto) e Ocimum basilicum L. (manjericão), sem e com incorporação de cama-de-frango semidecomposta. Os fatores em estudo foram a fáfia (F), o cravo-de-defunto (C) e o manjericão (M) em monocultivos e os policultivos com duas fileiras de fáfia e três de cravo (F2C3) e duas fileiras de fáfia e três de manjericão (F2M3), todos sem e com incorporação ao solo de cama-de-frango de corte semidecomposta. Os dez tratamentos foram arranjados no delineamento blocos casualizados, com quatro repetições. A produção de massa fresca e seca da parte aérea da fáfia foi maior (13,22 t ha-1 e 4,39 t ha-1, respectivamente) em monocultivo, independente do uso da cama-de-frango. Por outro lado, nenhum dos tratamentos influenciou a massa fresca e seca e o número de raízes da fáfia, que foram, em média, de 10,02 e 2,07 t ha-1 e 417.916 raízes ha-1, respectivamente. O diâmetro das raízes foi maior (23,5 mm) no policultivo com o manjericão. As produções de massas frescas e secas dos capítulos florais do cravo-de-defunto foram maiores (14,28 t ha-1e 1,278 t ha-1, respectivamente) no policultivo com a fáfia, mas apenas a produção de massa fresca dos capítulos foi maior (14,17 t ha-1) com o uso da cama-de-frango. A produção de parte aérea do manjericão foram maiores (52,91 t ha-1) no policultivo, independente com qual espécie; porém, não foi influenciada pelo uso da cama-de-frango. A razão de área equivalente (RAE) para o policultivo da fáfia com o cravo-de-defunto foi de 2,15 com cama-de-frango e de 1,99 sem cama e com o manjericão, foi 2,44 com cama e de 3,08 sem cama. Como os valores foram maiores que 1,0, indicam que os policultivos foram efetivos.<br>This study was carried out in field of the Universidade Federal da Grande Dourados, in Dourados, Brazil, during the period from March 2005 to September 2006. The aim of the experiment was to evaluate Pfaffia glomerata (Spreng) Pedersen yield under monocropping system or intercropped with Tagetes erecta L. and Ocimum basilicum L, in a Distroferric Red Latosol (sand loam Rhodic Oxisol), using semi-decomposed poultry manure (PM). The study objects were Brazilian ginseng (BG), marigold (M) and basil (B) under monocropping and the intercropping of two Brazilian ginseng, three marigold (BG2M3) and three basil (BG2B3) lines, all of them with or without semi-decomposed poultry manure (PM) as fertilizer. Ten treatments were arranged in randomized blocks design, with four replications. Fresh and dry weight production from shoot of Brazilian ginseng were higher (13.22 t ha-1 and 4.39 t ha-1, respectively) under monocropping, independently of the use of poultry manure. Nevertheless, none of experimental designs influenced the dry and fresh weight production or root number of Brazilian ginseng which produced average values of 10.02 and 2.07 t ha-1 and 417,916 roots ha-1, respectively. Root diameter was higher (23.5 mm) under intercropping system with basil. Dry and fresh weight of marigold flowers were higher (14.28 t ha-1and 1.278 t ha-1, respectively) when intercropped with Brazilian ginseng, but only fresh weight of the flowers was increased (14.17 t ha-1) by poultry manure application. Basil shoot production was higher (52.91 t ha-1) when intercropped, independently of the used species; however, they were not influenced by the use of poultry manure. Land equivalent ration (LER) for the Brazilian ginseng intercropped with marigold was 2.15 under poultry manure application and 1.99 without it, and for the basil 2.44 under poultry manure application and 3.08 without it. Values higher than 1.0 indicate that the intercropping systems were effective

References genes for qRT-PCR in guaraná (Paullina cupana var. sorbilis)

Gene expression has been extensively studied in plant science research, mainly for the assessment of plant stress responses. Real-time-quantitative polymerase chain reaction (RT-qPCR) is an important tool for obtaining this information because it is a quick and easy technique to acquire a large amount of molecular data for both model and non-model plants. For a successful RT-qPCR analysis, gene expression should be carefully normalised. Genes involved in essential biological processes that exhibit constitutive expression are commonly selected as internal standards to normalise RT-qPCR experiments. In this study, the transcription profiles of 13 candidate reference genes for RT-qPCR were evaluated in three guarana cultivars (BRS-Amazonas, BRS-Maués and BRS-Luzéia) using different tissues (vegetative and fruit) in varying developmental stages. Two different algorithms, NormFinder and GeNorm, were utilised to assess gene stability. In general, the two algorithms did not select the same pairs of genes for all analysed conditions. For the largest group (the fruits of all cultivars), NormFinder selected the pair EF1A/UBQ, whereas GeNorm chose ACT/GAPDH as the best normalising genes. Thus, we recommend the use of at least four reference genes for the normalisation of gene expression in guarana plant studies. © 2015, Botanical Society of Sao Paulo