Association of Factor V Secretion with Protein Kinase B Signaling in Platelets from Horses with Atypical Equine Thrombasthenia.

BackgroundTwo congenital bleeding diatheses have been identified in Thoroughbred horses: Glanzmann thrombasthenia (GT) and a second, novel diathesis associated with abnormal platelet function in response to collagen and thrombin stimulation.Hypothesis/objectivesPlatelet dysfunction in horses with this second thrombasthenia results from a secretory defect.AnimalsTwo affected and 6 clinically normal horses.MethodsEx vivo study. Washed platelets were examined for (1) expression of the αIIb-β3 integrin; (2) fibrinogen binding capacity in response to ADP and thrombin; (3) secretion of dense and α-granules; (4) activation of the mammalian target of rapamycin (mTOR)-protein kinase B (AKT) signaling pathway; and (5) cellular distribution of phosphatidylinositol-4-phosphate-3-kinase, class 2B (PIK3C2B) and SH2 containing inositol-5'-phosphatase 1 (SHIP1).ResultsPlatelets from affected horses expressed normal amounts of αIIb-β3 integrin and bound fibrinogen normally in response to ADP, but bound 80% less fibrinogen in response to thrombin. α-granules only released 50% as much Factor V as control platelets, but dense granules released their contents normally. Protein kinase B (AKT) phosphorylation was reduced after thrombin activation, but mTOR Complex 2 (mTORC2) and phosphoinositide-dependent kinase 1 (PDK1) signaling were normal. SH2-containing inositol-5'-phosphatase 1 (SHIP1) did not localize to the cytoskeleton of affected platelets and was decreased overall consistent with reduced AKT phosphorylation.Conclusions and clinical significanceDefects in fibrinogen binding, granule secretion, and signal transduction are unique to this thrombasthenia, which we designate as atypical equine thrombasthenia

On the galloping instability of two-dimensional bodies having elliptical cross sections.

Galloping, also known as Den Hartog instability, is the large amplitude, low frequency oscillation of a structure in the direction transverse to the mean wind direction. It normally appears in the case of bodies with small stiffness and structural damping, when they are placed in a flow provided the incident velocity is high enough. Galloping depends on the slope of the lift coefficient versus angle of attack curve, which must be negative. Generally speaking this implies that the body is stalled after boundary layer separation, which, as it is known in non-wedged bodies, is a Reynolds number dependent phenomenon. Wind tunnel experiments have been conducted aiming at establishing the characteristics of the galloping motion of elliptical cross-section bodies when subjected to a uniform flow, the angles of attack ranging from 0° to 90°. The results have been summarized in stability maps, both in the angle of attack versus relative thickness and in the angle of attack versus Reynolds number planes, where galloping instability regions are identified

Effects of boron and light treatments on the ascorbate concentration of alfalfa sprouts [abstract]

Abstract only availableAlfalfa (Medicago sativa) sprouts are an inexpensive, year-round source of fresh produce. Consumption of sprouts could be further advocated if their nutritional benefits were improved. The purpose of this study was to determine if growing alfalfa sprouts with boron and light treatments will increase the ascorbic acid (vitamin C) content of the sprouts. Alfalfa seeds were germinated for 3 days with and without boron. Both +boron and -boron treatments were grown in the dark or given a 3 hour light treatment. Dark germination of alfalfa with boron increased the ascorbate content of the sprouts by 30% compared to those grown without boron. Light treatment increased ascorbate in both +boron and -boron treatments, but boron did not seem to cause a significant difference in ascorbate among the light treated sprouts. These findings may present a method for increasing the ascorbic acid concentration in dark grown sprouts by germination in the presence of boron.Missouri Fertilizer and Lime Boar

High phosphorus rapidly increase squash root magnesium, sodium and calcium concentration

Abstract only availablePrevious studies in our laboratory have shown a significant impact of phosphorus nutrition on mineral element dynamics in plant roots and shoots (Reinbott and Blevins 1991, 1994, 1999). We hypothesize that increased P availability increases ATP concentration, driving rapid increases in root concentration of several cations by stimulating the proton pump and opening gated ion channels. Considering the rapid turnover of ATP in plant tissues, P-induced changes in root mineral element concentrations should be observed in a relatively short time. Therefore, experiments were designed to evaluate the effect of P on root tissue cation concentrations over the time course of 30 min to 8 hrs. Squash plants were grown hydroponically in a complete nutrient solution (500 µM P) for six days, following by four days with 50 µM P. Treatments of 50 and 500 & µM P were started on day 10 and roots were harvested after 0, 0.5, 1, 2, 4, and 8 hours. Roots were rinsed in DI H2O, blotted, frozen in liquid nitrogen, and freeze-dried. Freeze-dried samples were ground, digested in nitric acid using a closed-vessel microwave system, and macro- and micronutrient concentrations were determined using ICP-OES. As expected, higher P in nutrient solution resulted in higher P concentrations in squash roots. This increase was first observed after 2 hrs. As for other cations, high P increased leaf magnesium, sodium, and calcium concentrations, and decreased zinc and potassium concentrations. Interestingly, the change in concentration of all these elements was observed within 30 min. This is, to our knowledge, the first study showing a short-time impact of P nutrition on root cation composition. It supports the hypothesis of the role of P in cation uptake.Gyeongsang National Universit

Energy harvesting from transverse galloping

Some elastic bluff bodies under the action of a fluid flow can experience transverse galloping and lose stability if the flow velocity exceeds a critical value. For flow velocities higher than this critical value, there is an energy transfer from the flow to the body and the body develops an oscillatory motion. Usually, it is considered as an undesirable effect for civil or marine structures but here we will show that if the vibration is substantial, it can be used to extract useful energy from the surrounding flow. This paper explores analytically the potential use of transverse galloping in order to obtain energy. To this end, transverse galloping is described by a one-degree-of-freedom model where fluid forces obey the quasi-steady hypothesis. The influence of cross-section geometry and mechanical properties in the energy conversion factor is investigated

Observations on the vibration of axially-tensioned elastomeric pipes conveying fluids

A study of the effect of axial tension on the vibration of a single-span elastomeric pipe clamped at both ends conveying fluid has been carried out both experimentally and theoretically. A new mathematical model using a penalty function technique and the method of kinematic correction and fictitious loads has been developed. The influence of flowing fluid and axial tension on natural frequencies and mode shapes of the system has been described using this model and compared with experimental observations. Linear and non-linear dynamic response of the harmonically excited pipe has also been investigated for varying flow velocities and initial axial tensions

Nutrient interaction effects on yield and chemical composition of spinach and green beans

This report is based upon a thesis submitted by Mr. Blevins as partial fulfillment of a master of science degree from the College of Agriculture, University of Missouri-Columbia--P. [2].Digitized 2007 AES MoU.Includes bibliographical references (pages 22-23)

Susceptibility Provision Enhances Effective De-escalation (SPEED): utilizing rapid phenotypic susceptibility testing in Gram-negative bloodstream infections and its potential clinical impact

Abstract Objectives We evaluated the performance and time to result for pathogen identification (ID) and antimicrobial susceptibility testing (AST) of the Accelerate Pheno™ system (AXDX) compared with standard of care (SOC) methods. We also assessed the hypothetical improvement in antibiotic utilization if AXDX had been implemented. Methods Clinical samples from patients with monomicrobial Gram-negative bacteraemia were tested and compared between AXDX and the SOC methods of the VERIGENE® and Bruker MALDI Biotyper® systems for ID and the VITEK® 2 system for AST. Additionally, charts were reviewed to calculate theoretical times to antibiotic de-escalation, escalation and active and optimal therapy Results ID mean time was 21 h for MALDI-TOF MS, 4.4 h for VERIGENE® and 3.7 h for AXDX. AST mean time was 35 h for VITEK® 2 and 9.0 h for AXDX. For ID, positive percentage agreement was 95.9% and negative percentage agreement was 99.9%. For AST, essential agreement was 94.5% and categorical agreement was 93.5%. If AXDX results had been available to inform patient care, 25% of patients could have been put on active therapy sooner, while 78% of patients who had therapy optimized during hospitalization could have had therapy optimized sooner. Additionally, AXDX could have reduced time to de-escalation (16 versus 31 h) and escalation (19 versus 31 h) compared with SOC. Conclusions By providing fast and reliable ID and AST results, AXDX has the potential to improve antimicrobial utilization and enhance antimicrobial stewardship

CD 615 XL Discipleship Development in the Family

A study of the relationship of the church to the home in the joint enterprise of strengthening the family, nurturing children and bringing them to mature Christian discipleship. Special attention is given to the contemporary threats to family life and the emerging needs of the family.https://place.asburyseminary.edu/syllabi/3310/thumbnail.jp